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. 1983 Sep-Dec;56(5-6):405–411.

Phase separation, ion permeability, and the isolation of membranes from osmotically stable mycoplasmas.

S Rottem, M H Shirvan, Z Gross
PMCID: PMC2590543  PMID: 6089452

Abstract

The osmotic stability of M. gallisepticum was found to be a consequence of the synthesis of disaturated phosphatidylcholine incorporated into the cell membrane. The disaturated lipid induces the formation of segregated lipid domains, thus providing the sites for increased permeation of ions. Such permeation reduces the internal pressure so as to minimize cell swelling and subsequent lysis in a hypotonic medium. Purified membranes of M. gallisepticum can be prepared from cells suspended in an iso-osmotic NaCl solution containing either dicyclohexylcarbodiimide (DCCD), which blocks ATPase activity, or a mild alkaline buffer. Both conditions seem to interfere with cell volume regulation. These procedures can be used also to isolate membranes of other osmotically stable mycoplasmas.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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