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. 1991 Dec;59(12):4665–4669. doi: 10.1128/iai.59.12.4665-4669.1991

Isolation and sequence analysis of the Chlamydia pneumoniae GroE operon.

L C Kikuta 1, M Puolakkainen 1, C C Kuo 1, L A Campbell 1
PMCID: PMC259093  PMID: 1682266

Abstract

Chlamydia pneumoniae has emerged as an important human respiratory pathogen. From a lambda gt11 gene bank constructed from C. pneumoniae isolate AR-39 DNA, an immunoreactive plaque containing a 3.0-kb insert was purified. In immunoblots, a 60-kDa protein was recognized by anti-C. pneumoniae rabbit immune serum. The recombinant protein was reactive with a Chlamydia genus-specific monoclonal antibody recognizing a 60-kDa protein found in the Sarkosyl-soluble fraction and with rabbit immune serum prepared against the Chlamydia trachomatis 60-kDa GroEL homolog associated with the delayed-type hypersensitivity response. DNA sequence analysis confirmed that the C. pneumoniae gene product is an analog of the C. trachomatis delayed-type hypersensitivity antigen and the Escherichia coli GroEL heat shock protein.

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Selected References

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