FIGURE 3.

Effect of β-catenin/TCF transcriptional activity on the expression of SRp20 protein and transcript. DLD-1 cells were transfected with βCat-CA, empty pEGFP vector, or TCF-DN as indicated and then analyzed by Western blot for (upper panel) either SR protein levels or (middle panel) SRp20 protein level, and by RT-PCR for (lower panel) SRp20 transcript levels. Images from three different SRp20 Western blots as well as from RT-PCR experiments were digitalized, the band intensities were quantified, and the mean fold-changes are displayed graphically beside the corresponding panels.