Abstract
Immunoblotting with polyclonal rabbit antibodies raised against pilins expressed by Pseudomonas aeruginosa, Moraxella bovis, Neisseria gonorrhoeae, Dichelobacter nodosus, and Vibrio cholerae was used to demonstrate that these polypeptides display conserved antigenic and, in most cases, immunogenic determinants. These determinants appear to be localized to the highly homologous amino-terminal domains (residues 1 to 25).
Full text
PDF


Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Beard M. K., Mattick J. S., Moore L. J., Mott M. R., Marrs C. F., Egerton J. R. Morphogenetic expression of Moraxella bovis fimbriae (pili) in Pseudomonas aeruginosa. J Bacteriol. 1990 May;172(5):2601–2607. doi: 10.1128/jb.172.5.2601-2607.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Blake M. S., Johnston K. H., Russell-Jones G. J., Gotschlich E. C. A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots. Anal Biochem. 1984 Jan;136(1):175–179. doi: 10.1016/0003-2697(84)90320-8. [DOI] [PubMed] [Google Scholar]
- Dalrymple B., Mattick J. S. An analysis of the organization and evolution of type 4 fimbrial (MePhe) subunit proteins. J Mol Evol. 1987;25(3):261–269. doi: 10.1007/BF02100020. [DOI] [PubMed] [Google Scholar]
- Dewhirst F. E., Paster B. J., La Fontaine S., Rood J. I. Transfer of Kingella indologenes (Snell and Lapage 1976) to the genus Suttonella gen. nov. as Suttonella indologenes comb. nov.; transfer of Bacteroides nodosus (Beveridge 1941) to the genus Dichelobacter gen. nov. as Dichelobacter nodosus comb. nov.; and assignment of the genera Cardiobacterium, Dichelobacter, and Suttonella to Cardiobacteriaceae fam. nov. in the gamma division of Proteobacteria on the basis of 16S rRNA sequence comparisons. Int J Syst Bacteriol. 1990 Oct;40(4):426–433. doi: 10.1099/00207713-40-4-426. [DOI] [PubMed] [Google Scholar]
- Elleman T. C., Hoyne P. A., Stewart D. J., McKern N. M., Peterson J. E. Expression of pili from Bacteroides nodosus in Pseudomonas aeruginosa. J Bacteriol. 1986 Nov;168(2):574–580. doi: 10.1128/jb.168.2.574-580.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Froholm L. O., Sletten K. Purification and N-terminal sequence of a fimbrial protein from Moraxella nonliquefaciens. FEBS Lett. 1977 Jan 15;73(1):29–32. doi: 10.1016/0014-5793(77)80008-2. [DOI] [PubMed] [Google Scholar]
- Hermodson M. A., Chen K. C., Buchanan T. M. Neisseria pili proteins: amino-terminal amino acid sequences and identification of an unusual amino acid. Biochemistry. 1978 Feb 7;17(3):442–445. doi: 10.1021/bi00596a010. [DOI] [PubMed] [Google Scholar]
- Koomey M., Bergstrom S., Blake M., Swanson J. Pilin expression and processing in pilus mutants of Neisseria gonorrhoeae: critical role of Gly-1 in assembly. Mol Microbiol. 1991 Feb;5(2):279–287. doi: 10.1111/j.1365-2958.1991.tb02108.x. [DOI] [PubMed] [Google Scholar]
- Marrs C. F., Schoolnik G., Koomey J. M., Hardy J., Rothbard J., Falkow S. Cloning and sequencing of a Moraxella bovis pilin gene. J Bacteriol. 1985 Jul;163(1):132–139. doi: 10.1128/jb.163.1.132-139.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mattick J. S., Anderson B. J., Mott M. R., Egerton J. R. Isolation and characterization of Bacteroides nodosus fimbriae: structural subunit and basal protein antigens. J Bacteriol. 1984 Nov;160(2):740–747. doi: 10.1128/jb.160.2.740-747.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mattick J. S., Bills M. M., Anderson B. J., Dalrymple B., Mott M. R., Egerton J. R. Morphogenetic expression of Bacteroides nodosus fimbriae in Pseudomonas aeruginosa. J Bacteriol. 1987 Jan;169(1):33–41. doi: 10.1128/jb.169.1.33-41.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McKern N. M., O'Donnell I. J., Inglis A. S., Stewart D. J., Clark B. L. Amino acid sequence of pilin from Bacteroides nodosus (strain 198), the causative organism of ovine footrot. FEBS Lett. 1983 Nov 28;164(1):149–153. doi: 10.1016/0014-5793(83)80039-8. [DOI] [PubMed] [Google Scholar]
- Ruehl W. W., Marrs C. F., Fernandez R., Falkow S., Schoolnik G. K. Purification, characterization, and pathogenicity of Moraxella bovis pili. J Exp Med. 1988 Sep 1;168(3):983–1002. doi: 10.1084/jem.168.3.983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sastry P. A., Finlay B. B., Pasloske B. L., Paranchych W., Pearlstone J. R., Smillie L. B. Comparative studies of the amino acid and nucleotide sequences of pilin derived from Pseudomonas aeruginosa PAK and PAO. J Bacteriol. 1985 Nov;164(2):571–577. doi: 10.1128/jb.164.2.571-577.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shaw C. E., Taylor R. K. Vibrio cholerae O395 tcpA pilin gene sequence and comparison of predicted protein structural features to those of type 4 pilins. Infect Immun. 1990 Sep;58(9):3042–3049. doi: 10.1128/iai.58.9.3042-3049.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Strom M. S., Lory S. Cloning and expression of the pilin gene of Pseudomonas aeruginosa PAK in Escherichia coli. J Bacteriol. 1986 Feb;165(2):367–372. doi: 10.1128/jb.165.2.367-372.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Taylor R. K., Miller V. L., Furlong D. B., Mekalanos J. J. Use of phoA gene fusions to identify a pilus colonization factor coordinately regulated with cholera toxin. Proc Natl Acad Sci U S A. 1987 May;84(9):2833–2837. doi: 10.1073/pnas.84.9.2833. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Watts T. H., Sastry P. A., Hodges R. S., Paranchych W. Mapping of the antigenic determinants of Pseudomonas aeruginosa PAK polar pili. Infect Immun. 1983 Oct;42(1):113–121. doi: 10.1128/iai.42.1.113-121.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]