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. 1991 Dec;59(12):4681–4683. doi: 10.1128/iai.59.12.4681-4683.1991

Role of the galactose lectin of Entamoeba histolytica in adherence-dependent killing of mammalian cells.

L D Saffer 1, W A Petri Jr 1
PMCID: PMC259097  PMID: 1937828

Abstract

Entamoeba histolytica extracellular killing of host cells is contact dependent. Adherence to human colonic epithelial cells and mucins is mediated by a galactose-specific lectin. The effect on cytotoxicity of a panel of monoclonal antibodies (MAb) directed against the galactose lectin was tested. As expected, those MAb which inhibited adherence also decreased cytotoxicity. However, one antilectin MAb blocked cytotoxicity after adherence had occurred, indicating that the lectin has a role in cell killing that is distinct from its adherence function.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bailey G. B., Nudelman E. D., Day D. B., Harper C. F., Gilmour J. R. Specificity of glycosphingolipid recognition by Entamoeba histolytica trophozoites. Infect Immun. 1990 Jan;58(1):43–47. doi: 10.1128/iai.58.1.43-47.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Mann B. J., Torian B. E., Vedvick T. S., Petri W. A., Jr Sequence of a cysteine-rich galactose-specific lectin of Entamoeba histolytica. Proc Natl Acad Sci U S A. 1991 Apr 15;88(8):3248–3252. doi: 10.1073/pnas.88.8.3248. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Petri W. A., Jr, Chapman M. D., Snodgrass T., Mann B. J., Broman J., Ravdin J. I. Subunit structure of the galactose and N-acetyl-D-galactosamine-inhibitable adherence lectin of Entamoeba histolytica. J Biol Chem. 1989 Feb 15;264(5):3007–3012. [PubMed] [Google Scholar]
  4. Petri W. A., Jr, Joyce M. P., Broman J., Smith R. D., Murphy C. F., Ravdin J. I. Recognition of the galactose- or N-acetylgalactosamine-binding lectin of Entamoeba histolytica by human immune sera. Infect Immun. 1987 Oct;55(10):2327–2331. doi: 10.1128/iai.55.10.2327-2331.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Petri W. A., Jr, Ravdin J. I. Protection of gerbils from amebic liver abscess by immunization with the galactose-specific adherence lectin of Entamoeba histolytica. Infect Immun. 1991 Jan;59(1):97–101. doi: 10.1128/iai.59.1.97-101.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Petri W. A., Jr, Smith R. D., Schlesinger P. H., Murphy C. F., Ravdin J. I. Isolation of the galactose-binding lectin that mediates the in vitro adherence of Entamoeba histolytica. J Clin Invest. 1987 Nov;80(5):1238–1244. doi: 10.1172/JCI113198. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Petri W. A., Jr, Snodgrass T. L., Jackson T. F., Gathiram V., Simjee A. E., Chadee K., Chapman M. D. Monoclonal antibodies directed against the galactose-binding lectin of Entamoeba histolytica enhance adherence. J Immunol. 1990 Jun 15;144(12):4803–4809. [PubMed] [Google Scholar]
  8. Ravdin J. I., Croft B. Y., Guerrant R. L. Cytopathogenic mechanisms of Entamoeba histolytica. J Exp Med. 1980 Aug 1;152(2):377–390. doi: 10.1084/jem.152.2.377. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ravdin J. I., Guerrant R. L. Role of adherence in cytopathogenic mechanisms of Entamoeba histolytica. Study with mammalian tissue culture cells and human erythrocytes. J Clin Invest. 1981 Nov;68(5):1305–1313. doi: 10.1172/JCI110377. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ravdin J. I., Jackson T. F., Petri W. A., Jr, Murphy C. F., Ungar B. L., Gathiram V., Skilogiannis J., Simjee A. E. Association of serum antibodies to adherence lectin with invasive amebiasis and asymptomatic infection with pathogenic Entamoeba histolytica. J Infect Dis. 1990 Sep;162(3):768–772. doi: 10.1093/infdis/162.3.768. [DOI] [PubMed] [Google Scholar]
  11. Ravdin J. I., Moreau F., Sullivan J. A., Petri W. A., Jr, Mandell G. L. Relationship of free intracellular calcium to the cytolytic activity of Entamoeba histolytica. Infect Immun. 1988 Jun;56(6):1505–1512. doi: 10.1128/iai.56.6.1505-1512.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ravdin J. I., Schlesinger P. H., Murphy C. F., Gluzman I. Y., Krogstad D. J. Acid intracellular vesicles and the cytolysis of mammalian target cells by Entamoeba histolytica trophozoites. J Protozool. 1986 Nov;33(4):478–486. doi: 10.1111/j.1550-7408.1986.tb05646.x. [DOI] [PubMed] [Google Scholar]
  13. Saffer L. D., Petri W. A., Jr Entamoeba histolytica: recognition of alpha- and beta-galactose by the 260-kDa adherence lectin. Exp Parasitol. 1991 Jan;72(1):106–108. doi: 10.1016/0014-4894(91)90128-j. [DOI] [PubMed] [Google Scholar]
  14. Tannich E., Ebert F., Horstmann R. D. Primary structure of the 170-kDa surface lectin of pathogenic Entamoeba histolytica. Proc Natl Acad Sci U S A. 1991 Mar 1;88(5):1849–1853. doi: 10.1073/pnas.88.5.1849. [DOI] [PMC free article] [PubMed] [Google Scholar]

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