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. 1991 Dec;59(12):4724–4728. doi: 10.1128/iai.59.12.4724-4728.1991

Inability to express fimbriae results in impaired ability of Haemophilus influenzae b to colonize the nasopharynx.

A Weber 1, K Harris 1, S Lohrke 1, L Forney 1, A L Smith 1
PMCID: PMC259107  PMID: 1682268

Abstract

We cloned into the structural fimbrial subunit gene from a fimbriated Haemophilus influenzae b a 1.5-kb kanamycin resistance gene. The resultant strain (RKAW5) was tested by Southern analysis, hemagglutination, and electron-micrographic examination to confirm gene inactivation. In comparison with the parent, RKAW5 exhibited a significant decrease in adherence to human buccal epithelial cells and in nasal colonization of yearling rhesus monkeys.

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Selected References

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  1. Anderson P. W., Pichichero M. E., Connor E. M. Enhanced nasopharyngeal colonization of rats by piliated Haemophilus influenzae type b. Infect Immun. 1985 May;48(2):565–568. doi: 10.1128/iai.48.2.565-568.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Anderson P., Johnston R. B., Jr, Smith D. H. Human serum activities against Hemophilus influenzae, type b. J Clin Invest. 1972 Jan;51(1):31–38. doi: 10.1172/JCI106793. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Armes L. G., Forney L. J. The complete primary structure of pilin from Haemophilus influenzae type b strain Eagan. J Protein Chem. 1990 Feb;9(1):45–52. doi: 10.1007/BF01024983. [DOI] [PubMed] [Google Scholar]
  4. Chanyangam M., Smith A. L., Moseley S. L., Kuehn M., Jenny P. Contribution of a 28-kilodalton membrane protein to the virulence of Haemophilus influenzae. Infect Immun. 1991 Feb;59(2):600–608. doi: 10.1128/iai.59.2.600-608.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Connor E. M., Loeb M. R. A hemadsorption method for detection of colonies of Haemophilus influenzae type b expressing fimbriae. J Infect Dis. 1983 Nov;148(5):855–860. doi: 10.1093/infdis/148.5.855. [DOI] [PubMed] [Google Scholar]
  6. Darveau R. P., Hancock R. E. Procedure for isolation of bacterial lipopolysaccharides from both smooth and rough Pseudomonas aeruginosa and Salmonella typhimurium strains. J Bacteriol. 1983 Aug;155(2):831–838. doi: 10.1128/jb.155.2.831-838.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Farley M. M., Stephens D. S., Kaplan S. L., Mason E. O., Jr Pilus- and non-pilus-mediated interactions of Haemophilus influenzae type b with human erythrocytes and human nasopharyngeal mucosa. J Infect Dis. 1990 Feb;161(2):274–280. doi: 10.1093/infdis/161.2.274. [DOI] [PubMed] [Google Scholar]
  8. Forney L. J., Marrs C. F., Bektesh S. L., Gilsdorf J. R. Comparison and analysis of the nucleotide sequences of pilin genes from Haemophilus influenzae type b strains Eagan and M43. Infect Immun. 1991 Jun;59(6):1991–1996. doi: 10.1128/iai.59.6.1991-1996.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gilsdorf J. R., Ferrieri P. Adherence of Haemophilus influenzae to human epithelial cells. Scand J Infect Dis. 1984;16(3):271–278. doi: 10.3109/00365548409070400. [DOI] [PubMed] [Google Scholar]
  10. Guerina N. G., Langermann S., Clegg H. W., Kessler T. W., Goldman D. A., Gilsdorf J. R. Adherence of piliated Haemophilus influenzae type b to human oropharyngeal cells. J Infect Dis. 1982 Oct;146(4):564–564. doi: 10.1093/infdis/146.4.564. [DOI] [PubMed] [Google Scholar]
  11. Herriott R. M., Meyer E. M., Vogt M. Defined nongrowth media for stage II development of competence in Haemophilus influenzae. J Bacteriol. 1970 Feb;101(2):517–524. doi: 10.1128/jb.101.2.517-524.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hull R. A., Gill R. E., Hsu P., Minshew B. H., Falkow S. Construction and expression of recombinant plasmids encoding type 1 or D-mannose-resistant pili from a urinary tract infection Escherichia coli isolate. Infect Immun. 1981 Sep;33(3):933–938. doi: 10.1128/iai.33.3.933-938.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kaplan S. L., Mason E. O., Jr, Wiedermann B. L. Role of adherence in the pathogenesis of Haemophilus influenzae type b infection in infant rats. Infect Immun. 1983 Nov;42(2):612–617. doi: 10.1128/iai.42.2.612-617.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lampe R. M., Mason E. O., Jr, Kaplan S. L., Umstead C. L., Yow M. D., Feigin R. D. Adherence of Haemophilus influenzae to buccal epithelial cells. Infect Immun. 1982 Jan;35(1):166–172. doi: 10.1128/iai.35.1.166-172.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Loeb M. R., Connor E., Penney D. A comparison of the adherence of fimbriated and nonfimbriated Haemophilus influenzae type b to human adenoids in organ culture. Infect Immun. 1988 Feb;56(2):484–489. doi: 10.1128/iai.56.2.484-489.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Mason E. O., Jr, Kaplan S. L., Wiedermann B. L., Norrod E. P., Stenback W. A. Frequency and properties of naturally occurring adherent piliated strains of Haemophilus influenzae type b. Infect Immun. 1985 Jul;49(1):98–103. doi: 10.1128/iai.49.1.98-103.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Oka A., Sugisaki H., Takanami M. Nucleotide sequence of the kanamycin resistance transposon Tn903. J Mol Biol. 1981 Apr 5;147(2):217–226. doi: 10.1016/0022-2836(81)90438-1. [DOI] [PubMed] [Google Scholar]
  18. Orndorff P. E., Bloch C. A. The role of type 1 pili in the pathogenesis of Escherichia coli infections: a short review and some new ideas. Microb Pathog. 1990 Aug;9(2):75–79. doi: 10.1016/0882-4010(90)90081-z. [DOI] [PubMed] [Google Scholar]
  19. Pichichero M. E. Adherence of Haemophilus influenzae to human buccal and pharyngeal epithelial cells: relationship to pilation. J Med Microbiol. 1984 Aug;18(1):107–116. doi: 10.1099/00222615-18-1-107. [DOI] [PubMed] [Google Scholar]
  20. Pichichero M. E., Loeb M., Anderson, Smith D. H. Do pili play a role in pathogenicity of Haemophilus influenzae type B? Lancet. 1982 Oct 30;2(8305):960–962. doi: 10.1016/s0140-6736(82)90161-1. [DOI] [PubMed] [Google Scholar]
  21. Sable N. S., Connor E. M., Hall C. B., Loeb M. R. Variable adherence of fimbriated Haemophilus influenzae type b to human cells. Infect Immun. 1985 Apr;48(1):119–123. doi: 10.1128/iai.48.1.119-123.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Scheifele D. W., Daum R. S., Syriopoulou V. P., Averill D. R., Smith A. L. Haemophilus influenzae bacteremia and meningitis in infant primates. J Lab Clin Med. 1980 Mar;95(3):450–462. [PubMed] [Google Scholar]
  23. Smith A. L., Smith D. H., Averill D. R., Jr, Marino J., Moxon E. R. Production of Haemophilus influenzae b meningitis in infant rats by intraperitoneal inoculation. Infect Immun. 1973 Aug;8(2):278–290. doi: 10.1128/iai.8.2.278-290.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Stull T. L., Mendelman P. M., Haas J. E., Schoenborn M. A., Mack K. D., Smith A. L. Characterization of Haemophilus influenzae type b fimbriae. Infect Immun. 1984 Dec;46(3):787–796. doi: 10.1128/iai.46.3.787-796.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Vieira J., Messing J. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene. 1982 Oct;19(3):259–268. doi: 10.1016/0378-1119(82)90015-4. [DOI] [PubMed] [Google Scholar]
  26. Wong K., Roberts M. C., Owens L., Fife M., Smith A. L. Selective media for the quantitation of bacteria in cystic fibrosis sputum. J Med Microbiol. 1984 Apr;17(2):113–119. doi: 10.1099/00222615-17-2-113. [DOI] [PubMed] [Google Scholar]
  27. de Groot R., Campos J., Moseley S. L., Smith A. L. Molecular cloning and mechanism of trimethoprim resistance in Haemophilus influenzae. Antimicrob Agents Chemother. 1988 Apr;32(4):477–484. doi: 10.1128/aac.32.4.477. [DOI] [PMC free article] [PubMed] [Google Scholar]

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