Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1988 Jan;56(1):99–105. doi: 10.1128/iai.56.1.99-105.1988

Mucosal and systemic immunity to Campylobacter jejuni in rabbits after gastric inoculation.

D H Burr 1, M B Caldwell 1, A L Bourgeois 1, H R Morgan 1, R Wistar Jr 1, R I Walker 1
PMCID: PMC259241  PMID: 3335413

Abstract

The mucosal and systemic immune responses to Campylobacter jejuni were studied in rabbits receiving gastric inoculation with live organisms. A lavage procedure was used to facilitate repeated monitoring of the intestinal immune response to C. jejuni. Immunity to C. jejuni was determined by secondary challenge by using the removable intestinal tie adult rabbit diarrhea (RITARD) model and monitoring for resistance to colonization and bacteremia. Oral-gastric inoculation of normal rabbits produced a transient intestinal colonization without diarrhea. C. jejuni serotypes differed in their ability to colonize the intestines of rabbits and to stimulate primary intestinal and serum antibody responses. Animals previously colonized were resistant to recolonization and the development of bacteremia after homologous challenge by the RITARD procedure but were not resistant to heterologous challenges. Anticampylobacter intestinal and serum IgA titers before this secondary infection were the most reliable predictors of resistance to colonization and bacteremia.

Full text

PDF
99

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blaser M. J., Black R. E., Duncan D. J., Amer J. Campylobacter jejuni-specific serum antibodies are elevated in healthy Bangladeshi children. J Clin Microbiol. 1985 Feb;21(2):164–167. doi: 10.1128/jcm.21.2.164-167.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Blaser M. J., Duncan D. J. Human serum antibody response to Campylobacter jejuni infection as measured in an enzyme-linked immunosorbent assay. Infect Immun. 1984 May;44(2):292–298. doi: 10.1128/iai.44.2.292-298.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Blaser M. J., Smith P. F., Kohler P. F. Susceptibility of Campylobacter isolates to the bactericidal activity of human serum. J Infect Dis. 1985 Feb;151(2):227–235. doi: 10.1093/infdis/151.2.227. [DOI] [PubMed] [Google Scholar]
  4. Blaser M. J., Taylor D. N., Echeverria P. Immune response to Campylobacter jejuni in a rural community in Thailand. J Infect Dis. 1986 Feb;153(2):249–254. doi: 10.1093/infdis/153.2.249. [DOI] [PubMed] [Google Scholar]
  5. Burr D. H., Kerner D. T., Blanco C. S., Bourgeois A. L., Wistar R., Jr Gastric lavage: a simple method to obtain IgA-rich intestinal secretions from the rabbit. J Immunol Methods. 1987 May 20;99(2):277–281. doi: 10.1016/0022-1759(87)90138-4. [DOI] [PubMed] [Google Scholar]
  6. Caldwell M. B., Walker R. I., Stewart S. D., Rogers J. E. Simple adult rabbit model for Campylobacter jejuni enteritis. Infect Immun. 1983 Dec;42(3):1176–1182. doi: 10.1128/iai.42.3.1176-1182.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cebra J. J., Robbins J. B. Gamma-A-immunoglobulin from rabbit colostrum. J Immunol. 1966 Jul;97(1):12–24. [PubMed] [Google Scholar]
  8. Cray W. C., Jr, Tokunaga E., Pierce N. F. Successful colonization and immunization of adult rabbits by oral inoculation with Vibrio cholerae O1. Infect Immun. 1983 Aug;41(2):735–741. doi: 10.1128/iai.41.2.735-741.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Fubara E. S., Freter R. Protection against enteric bacterial infection by secretory IgA antibodies. J Immunol. 1973 Aug;111(2):395–403. [PubMed] [Google Scholar]
  10. Glass R. I., Stoll B. J., Huq M. I., Struelens M. J., Blaser M., Kibriya A. K. Epidemiologic and clinical features of endemic Campylobacter jejuni infection in Bangladesh. J Infect Dis. 1983 Aug;148(2):292–296. doi: 10.1093/infdis/148.2.292. [DOI] [PubMed] [Google Scholar]
  11. Kaldor J., Pritchard H., Serpell A., Metcalf W. Serum antibodies in Campylobacter enteritis. J Clin Microbiol. 1983 Jul;18(1):1–4. doi: 10.1128/jcm.18.1.1-4.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Keren D. F., Collins H. H., Gemski P., Holt P. S., Formal S. B. Role of antigen form in development of mucosal immunoglobin A response to Shigella flexneri Antigens. Infect Immun. 1981 Mar;31(3):1193–1202. doi: 10.1128/iai.31.3.1193-1202.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lane E. M., Batchelor R. A., Bourgeois A. L., Burr D. H., Olson J. G. Urine and faecal IgA response during naturally acquired infection with Campylobacter jejuni. Lancet. 1987 May 16;1(8542):1141–1141. doi: 10.1016/s0140-6736(87)91694-1. [DOI] [PubMed] [Google Scholar]
  14. Logan S. M., Trust T. J. Location of epitopes on Campylobacter jejuni flagella. J Bacteriol. 1986 Nov;168(2):739–745. doi: 10.1128/jb.168.2.739-745.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Logan S. M., Trust T. J. Molecular identification of surface protein antigens of Campylobacter jejuni. Infect Immun. 1983 Nov;42(2):675–682. doi: 10.1128/iai.42.2.675-682.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. McNabb P. C., Tomasi T. B. Host defense mechanisms at mucosal surfaces. Annu Rev Microbiol. 1981;35:477–496. doi: 10.1146/annurev.mi.35.100181.002401. [DOI] [PubMed] [Google Scholar]
  17. McSweegan E., Burr D. H., Walker R. I. Intestinal mucus gel and secretory antibody are barriers to Campylobacter jejuni adherence to INT 407 cells. Infect Immun. 1987 Jun;55(6):1431–1435. doi: 10.1128/iai.55.6.1431-1435.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. McSweegan E., Walker R. I. Identification and characterization of two Campylobacter jejuni adhesins for cellular and mucous substrates. Infect Immun. 1986 Jul;53(1):141–148. doi: 10.1128/iai.53.1.141-148.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Melamed I., Bujanover Y., Igra Y. S., Schwartz D., Zakuth V., Spirer Z. Campylobacter enteritis in normal and immunodeficient children. Am J Dis Child. 1983 Aug;137(8):752–753. doi: 10.1001/archpedi.1983.02140340036009. [DOI] [PubMed] [Google Scholar]
  20. Pierce N. F., Kaper J. B., Mekalanos J. J., Cray W. C., Jr, Richardson K. Determinants of the immunogenicity of live virulent and mutant Vibrio cholerae O1 in rabbit intestine. Infect Immun. 1987 Feb;55(2):477–481. doi: 10.1128/iai.55.2.477-481.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Smith H. Microbial surfaces in relation to pathogenicity. Bacteriol Rev. 1977 Jun;41(2):475–500. doi: 10.1128/br.41.2.475-500.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Spira W. M., Sack R. B., Froehlich J. L. Simple adult rabbit model for Vibrio cholerae and enterotoxigenic Escherichia coli diarrhea. Infect Immun. 1981 May;32(2):739–747. doi: 10.1128/iai.32.2.739-747.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Stoll B. J., Svennerholm A. M., Gothefors L., Barua D., Huda S., Holmgren J. Local and systemic antibody responses to naturally acquired enterotoxigenic Escherichia coli diarrhea in an endemic area. J Infect Dis. 1986 Mar;153(3):527–534. doi: 10.1093/infdis/153.3.527. [DOI] [PubMed] [Google Scholar]
  24. Svennerholm A. M., Jertborn M., Gothefors L., Karim A. M., Sack D. A., Holmgren J. Mucosal antitoxic and antibacterial immunity after cholera disease and after immunization with a combined B subunit-whole cell vaccine. J Infect Dis. 1984 Jun;149(6):884–893. doi: 10.1093/infdis/149.6.884. [DOI] [PubMed] [Google Scholar]
  25. Walker R. I., Caldwell M. B., Lee E. C., Guerry P., Trust T. J., Ruiz-Palacios G. M. Pathophysiology of Campylobacter enteritis. Microbiol Rev. 1986 Mar;50(1):81–94. doi: 10.1128/mr.50.1.81-94.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Winsor D. K., Jr, Mathewson J. J., DuPont H. L. Western blot analysis of intestinal secretory immunoglobulin A response to Campylobacter jejuni antigens in patients with naturally acquired Campylobacter enteritis. Gastroenterology. 1986 May;90(5 Pt 1):1217–1222. doi: 10.1016/0016-5085(86)90388-4. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES