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. 1988 Jan;56(1):287–290. doi: 10.1128/iai.56.1.287-290.1988

Comparison of Chlamydia psittaci isolates by restriction endonuclease and DNA probe analyses.

P Timms 1, F W Eaves 1, A A Girjes 1, M F Lavin 1
PMCID: PMC259273  PMID: 2826336

Abstract

DNAs from eight Chlamydia psittaci isolates (koala conjunctivitis, avian psittacosis, avian ornithosis, ovine abortion, ovine polyarthritis, sporadic bovine encephalomyelitis, and feline conjunctivitis) and one Chlamydia trachomatis isolate (lymphogranuloma venereum) were compared by restriction endonuclease and DNA probe analyses. Digestion with HindIII yielded a series of discrete fragments which allowed the differentiation of most isolates. A gene probe, pFEN207, which encodes the chlamydia-specific component of the lipopolysaccharide group antigen was used in Southern hybridizations. The probe was chlamydia specific and hybridized to a single BamHI fragment and multiple HindIII fragments in each isolate. The variation in size of the hybridizing fragments allowed easy differentiation of the isolates and may eventually lead to a meaningful subgrouping of the diverse group of disease agents presently included in the species C. psittaci.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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