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. 1988 Feb;56(2):400–404. doi: 10.1128/iai.56.2.400-404.1988

Cross-neutralization of staphylococcal and streptococcal pyrogenic toxins by monoclonal and polyclonal antibodies.

G A Bohach 1, C J Hovde 1, J P Handley 1, P M Schlievert 1
PMCID: PMC259295  PMID: 2448242

Abstract

We evaluated cross-reactivity of antibodies against staphylococcal and streptococcal pyrogenic toxins. Monoclonal antibodies against staphylococcal enterotoxin (ET) C1 and streptococcal pyrogenic exotoxin (SPE) A were tested for reactivity with homologous and heterologous pyrogenic toxins in vitro. Ten immunoglobulin G1 anti-ET C1 monoclonal antibodies showed little or no cross-reactivity in an enzyme-linked immunosorbent assay, but many of these could neutralize the mitogenic effect of ET B, SPE A, or both. Two immunoglobulin M anti-ET C1 monoclonal antibodies and eight immunoglobulin M anti-SPE A monoclonal antibodies showed extensive cross-reactivity in the enzyme-linked immunosorbent assay and the mitogenicity neutralization assay. No cross-reactivity was observed with SPE C or toxic shock syndrome toxin 1. Rabbits immunized against ET B, ET C1, or SPE A were resistant to challenge with the immunizing toxin. In addition, reciprocal immunity was stimulated by the two ETs, and immunity to SPE A provided protection against ET B but not ET C1. These results show that staphylococcal and streptococcal pyrogenic toxins which share sequence homology have common antigenic determinants which may not be detected in Ouchterlony immunodiffusion assays.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Avena R. M., Bergdoll M. S. Purification and some physicochemical properties of enterotoxin C, Staphylococcus aureus strain 361. Biochemistry. 1967 May;6(5):1474–1480. doi: 10.1021/bi00857a033. [DOI] [PubMed] [Google Scholar]
  2. Barsumian E. L., Schlievert P. M., Watson D. W. Nonspecific and specific immunological mitogenicity by group A streptococcal pyrogenic exotoxins. Infect Immun. 1978 Dec;22(3):681–688. doi: 10.1128/iai.22.3.681-688.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bergdoll M. S., Borja C. R., Avena R. M. Identification of a new enterotoxin as enterotoxin C. J Bacteriol. 1965 Nov;90(5):1481–1485. doi: 10.1128/jb.90.5.1481-1485.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bergdoll M. S., Borja C. R., Robbins R. N., Weiss K. F. Identification of enterotoxin E. Infect Immun. 1971 Nov;4(5):593–595. doi: 10.1128/iai.4.5.593-595.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bergdoll M. S., Robbins R. N., Weiss K., Borja C. R., Huang Y., Chu F. S. The staphylococcal enterotoxins: similarities. Contrib Microbiol Immunol. 1973;1:390–396. [PubMed] [Google Scholar]
  6. Blomster-Hautamaa D. A., Kreiswirth B. N., Kornblum J. S., Novick R. P., Schlievert P. M. The nucleotide and partial amino acid sequence of toxic shock syndrome toxin-1. J Biol Chem. 1986 Nov 25;261(33):15783–15786. [PubMed] [Google Scholar]
  7. Blomster-Hautamaa D. A., Novick R. P., Schlievert P. M. Localization of biologic functions of toxic shock syndrome toxin-1 by use of monoclonal antibodies and cyanogen bromide-generated toxin fragments. J Immunol. 1986 Dec 1;137(11):3572–3576. [PubMed] [Google Scholar]
  8. Bohach G. A., Schlievert P. M. Expression of staphylococcal enterotoxin C1 in Escherichia coli. Infect Immun. 1987 Feb;55(2):428–432. doi: 10.1128/iai.55.2.428-432.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Bohach G. A., Schlievert P. M. Nucleotide sequence of the staphylococcal enterotoxin C1 gene and relatedness to other pyrogenic toxins. Mol Gen Genet. 1987 Aug;209(1):15–20. doi: 10.1007/BF00329830. [DOI] [PubMed] [Google Scholar]
  10. Brunson K. W., Watson D. W. Pyrogenic specificity of streptococcal exotoxins, staphylococcal enterotoxin, and gram-negative endotoxin. Infect Immun. 1974 Aug;10(2):347–351. doi: 10.1128/iai.10.2.347-351.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Casman E. P., Bennett R. W., Dorsey A. E., Issa J. A. Identification of a fourth staphylococcal enterotoxin, enterotoxin D. J Bacteriol. 1967 Dec;94(6):1875–1882. doi: 10.1128/jb.94.6.1875-1882.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hynes W. L., Weeks C. R., Iandolo J. J., Ferretti J. J. Immunologic cross-reactivity of type A streptococcal exotoxin (erythrogenic toxin) and staphylococcal enterotoxins B and C1. Infect Immun. 1987 Mar;55(3):837–838. doi: 10.1128/iai.55.3.837-838.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Johnson L. P., L'Italien J. J., Schlievert P. M. Streptococcal pyrogenic exotoxin type A (scarlet fever toxin) is related to Staphylococcus aureus enterotoxin B. Mol Gen Genet. 1986 May;203(2):354–356. doi: 10.1007/BF00333979. [DOI] [PubMed] [Google Scholar]
  14. Kearney J. F., Radbruch A., Liesegang B., Rajewsky K. A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines. J Immunol. 1979 Oct;123(4):1548–1550. [PubMed] [Google Scholar]
  15. Kim Y. B., Watson D. W. A purified group A streptococcal pyrogenic exotoxin. Physiochemical and biological properties including the enhancement of susceptibility to endotoxin lethal shock. J Exp Med. 1970 Mar 1;131(3):611–622. doi: 10.1084/jem.131.3.611. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Poindexter N. J., Schlievert P. M. Toxic-shock-syndrome toxin 1-induced proliferation of lymphocytes: comparison of the mitogenic response of human, murine, and rabbit lymphocytes. J Infect Dis. 1985 Jan;151(1):65–72. doi: 10.1093/infdis/151.1.65. [DOI] [PubMed] [Google Scholar]
  17. Reiser R. F., Robbins R. N., Noleto A. L., Khoe G. P., Bergdoll M. S. Identification, purification, and some physicochemical properties of staphylococcal enterotoxin C3. Infect Immun. 1984 Sep;45(3):625–630. doi: 10.1128/iai.45.3.625-630.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. SCHWAB J. H., WATSON D. W., CROMARTIE W. J. Further studies of group A streptococcal factors with lethal and cardiotoxic properties. J Infect Dis. 1955 Jan-Feb;96(1):14–18. doi: 10.1093/infdis/96.1.14. [DOI] [PubMed] [Google Scholar]
  19. SUGIYAMA H., MCKISSIC E. M., Jr, BERGDOLL M. S., HELLER B. ENHANCEMENT OF BACTERIAL ENDOTOXIN LETHALITY BY STAPHYLOCOCCAL ENTEROTOXIN. J Infect Dis. 1964 Apr;114:111–118. doi: 10.1093/infdis/114.2.111. [DOI] [PubMed] [Google Scholar]
  20. Schlievert P. M. Activation of murine T-suppressor lymphocytes by group A streptococcal and staphylococcal pyurogenic exotoxins. Infect Immun. 1980 Jun;28(3):876–880. doi: 10.1128/iai.28.3.876-880.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Schlievert P. M., Bettin K. M., Watson D. W. Purification and characterization of group A streptococcal pyrogenic exotoxin type C. Infect Immun. 1977 May;16(2):673–679. doi: 10.1128/iai.16.2.673-679.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Schlievert P. M., Blomster D. A. Production of staphylococcal pyrogenic exotoxin type C: influence of physical and chemical factors. J Infect Dis. 1983 Feb;147(2):236–242. doi: 10.1093/infdis/147.2.236. [DOI] [PubMed] [Google Scholar]
  23. Schlievert P. M., Schoettle D. J., Watson D. W. Purification and physicochemical and biological characterization of a staphylococcal pyrogenic exotoxin. Infect Immun. 1979 Mar;23(3):609–617. doi: 10.1128/iai.23.3.609-617.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Schlievert P. M., Shands K. N., Dan B. B., Schmid G. P., Nishimura R. D. Identification and characterization of an exotoxin from Staphylococcus aureus associated with toxic-shock syndrome. J Infect Dis. 1981 Apr;143(4):509–516. doi: 10.1093/infdis/143.4.509. [DOI] [PubMed] [Google Scholar]
  25. Schmidt J. J., Spero L. The complete amino acid sequence of staphylococcal enterotoxin C1. J Biol Chem. 1983 May 25;258(10):6300–6306. [PubMed] [Google Scholar]
  26. Smith B. G., Johnson H. M. The effect of staphylococcal enterotoxins on the primary in vitro immune response. J Immunol. 1975 Aug;115(2):575–578. [PubMed] [Google Scholar]
  27. Spero L., Morlock B. A. Cross-reactions between tryptic polypeptides of staphylococcal enterotoxins B and C. J Immunol. 1979 Apr;122(4):1285–1289. [PubMed] [Google Scholar]
  28. Spero L., Morlock B. A., Metzger J. F. On the cross-reactivity of staphylococcal enterotoxins A, B, and C. J Immunol. 1978 Jan;120(1):86–89. [PubMed] [Google Scholar]
  29. Weeks C. R., Ferretti J. J. Nucleotide sequence of the type A streptococcal exotoxin (erythrogenic toxin) gene from Streptococcus pyogenes bacteriophage T12. Infect Immun. 1986 Apr;52(1):144–150. doi: 10.1128/iai.52.1.144-150.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]

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