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. 1988 Mar;56(3):703–704. doi: 10.1128/iai.56.3.703-704.1988

Kinetics of proteolysis of hog gastric mucin by human neutrophil elastase and by Pseudomonas aeruginosa elastase.

L Poncz 1, N Jentoft 1, M C Ho 1, D G Dearborn 1
PMCID: PMC259349  PMID: 3125112

Abstract

Human neutrophil and Pseudomonas aeruginosa elastases were compared for their ability to degrade hog gastric mucin, which was used as a model substrate. P. aeruginosa elastase was more active than neutrophil elastase, and 2 to 10 peptide bonds were hydrolyzed within 5 min. The results demonstrate that both elastases degrade mucins actively at concentrations comparable to physiological levels of neutrophil elastase, which raises the possibility that proteolysis of mucins may be one mechanism of damage during chronic infection and inflammation of the respiratory tract.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boat T. F., Cheng P. W., Wood R. E. Tracheobronchial mucus secretion in vivo and in vitro by epithelial tissues from cystic fibrosis and control subjects. Mod Probl Paediatr. 1976 Oct 24;19:141–152. [PubMed] [Google Scholar]
  2. Bruce M. C., Poncz L., Klinger J. D., Stern R. C., Tomashefski J. F., Jr, Dearborn D. G. Biochemical and pathologic evidence for proteolytic destruction of lung connective tissue in cystic fibrosis. Am Rev Respir Dis. 1985 Sep;132(3):529–535. doi: 10.1164/arrd.1985.132.3.529. [DOI] [PubMed] [Google Scholar]
  3. Goldstein W., Döring G. Lysosomal enzymes from polymorphonuclear leukocytes and proteinase inhibitors in patients with cystic fibrosis. Am Rev Respir Dis. 1986 Jul;134(1):49–56. doi: 10.1164/arrd.1986.134.1.49. [DOI] [PubMed] [Google Scholar]
  4. Jackson A. H., Hill S. L., Afford S. C., Stockley R. A. Sputum sol-phase proteins and elastase activity in patients with cystic fibrosis. Eur J Respir Dis. 1984 Feb;65(2):114–124. [PubMed] [Google Scholar]
  5. Jentoft N., Dearborn D. G. Protein labeling by reductive alkylation. Methods Enzymol. 1983;91:570–579. doi: 10.1016/s0076-6879(83)91052-2. [DOI] [PubMed] [Google Scholar]
  6. Klinger J. D., Straus D. C., Hilton C. B., Bass J. A. Antibodies to proteases and exotoxin A of Pseudomonas aeruginosa in patients with cystic fibrosis: Demonstration by radioimmunoassay. J Infect Dis. 1978 Jul;138(1):49–48. doi: 10.1093/infdis/138.1.49. [DOI] [PubMed] [Google Scholar]
  7. Martodam R. R., Baugh R. J., Twumasi D. Y., Liener I. E. A rapid procedure for the large scale purification of elastase and cathepsin G from human sputum. Prep Biochem. 1979;9(1):15–31. doi: 10.1080/00327487908061669. [DOI] [PubMed] [Google Scholar]
  8. Poncz L., Dearborn D. G. The resistance to tryptic hydrolysis of peptide bonds adjacent to N epsilon,N-dimethyllysyl residues. J Biol Chem. 1983 Feb 10;258(3):1844–1850. [PubMed] [Google Scholar]
  9. Scawen M., Allen A. The action of proteolytic enzymes on the glycoprotein from pig gastric mucus. Biochem J. 1977 May 1;163(2):363–368. doi: 10.1042/bj1630363. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Shogren R. L., Jamieson A. M., Blackwell J., Jentoft N. Conformation of mucous glycoproteins in aqueous solvents. Biopolymers. 1986 Aug;25(8):1505–1517. doi: 10.1002/bip.360250809. [DOI] [PubMed] [Google Scholar]
  11. Snary D., Allen A. Studies on gastric mucoproteins. The production of radioactive mucoproteins by pig gastric mucosal scrapings in vitro. Biochem J. 1972 Apr;127(3):577–587. doi: 10.1042/bj1270577. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Stockley R. A., Burnett D. Serum derived protease inhibitors and leucocyte elastase in sputum and the effect of infection. Bull Eur Physiopathol Respir. 1980;16 (Suppl):261–272. doi: 10.1016/b978-0-08-027379-2.50027-2. [DOI] [PubMed] [Google Scholar]
  13. Suter S., Schaad U. B., Roux L., Nydegger U. E., Waldvogel F. A. Granulocyte neutral proteases and Pseudomonas elastase as possible causes of airway damage in patients with cystic fibrosis. J Infect Dis. 1984 Apr;149(4):523–531. doi: 10.1093/infdis/149.4.523. [DOI] [PubMed] [Google Scholar]
  14. Yasutake A., Powers J. C. Reactivity of human leukocyte elastase and porcine pancreatic elastase toward peptide 4-nitroanilides containing model desmosine residues. Evidence that human leukocyte elastase is selective for cross-linked regions of elastin. Biochemistry. 1981 Jun 23;20(13):3675–3679. doi: 10.1021/bi00516a002. [DOI] [PubMed] [Google Scholar]

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