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. 1988 Apr;56(4):773–778. doi: 10.1128/iai.56.4.773-778.1988

Purification and characterization of H.8 antigen from group B Neisseria meningitidis.

A K Bhattacharjee 1, E E Moran 1, J S Ray 1, W D Zollinger 1
PMCID: PMC259369  PMID: 2450067

Abstract

The surface antigen (H.8) common to the pathogenic Neisseria species was purified by a simple procedure by use of high-performance liquid chromatography. The purified H.8 antigen was characterized as to its amino acid composition, susceptibilities to several proteolytic enzymes, isoelectric point, and susceptibilities to an acid and a base. The amino acid composition of purified H.8 antigen from two strains of Neisseria meningitidis group B, namely, 44/76 and 8047, were compared. It was found that glutamic acid, alanine, and proline accounted for about 80% of the total amino acids in each case. A preliminary analysis of the lipid content of this protein was made. It showed the presence of a lipid component that moves between C9 and C11 straight-chain fatty acids in the gas chromatograph. Limited amino acid sequence data were obtained by sequencing a fragment of the H.8 antigen that was isolated after partial acid hydrolysis. The H.8 antigen epitope was found to be labile to treatment with both a mild acid and a mild base.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Black J. R., Black W. J., Cannon J. G. Neisserial antigen H.8 is immunogenic in patients with disseminated gonococcal and meningococcal infections. J Infect Dis. 1985 Apr;151(4):650–657. doi: 10.1093/infdis/151.4.650. [DOI] [PubMed] [Google Scholar]
  2. Black W. J., Cannon J. G. Cloning of the gene for the common pathogenic Neisseria H.8 antigen from Neisseria gonorrhoeae. Infect Immun. 1985 Jan;47(1):322–325. doi: 10.1128/iai.47.1.322-325.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cannon J. G., Black W. J., Nachamkin I., Stewart P. W. Monoclonal antibody that recognizes an outer membrane antigen common to the pathogenic Neisseria species but not to most nonpathogenic Neisseria species. Infect Immun. 1984 Mar;43(3):994–999. doi: 10.1128/iai.43.3.994-999.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Garner C. W., Jr, Behal F. J. Human liver aminopeptidase. Role of metal ions in mechanism of action. Biochemistry. 1974 Jul 30;13(16):3227–3233. doi: 10.1021/bi00713a005. [DOI] [PubMed] [Google Scholar]
  5. Gilles A. M., Imhoff J. M., Keil B. alpha-Clostripain. Chemical characterization, activity, and thiol content of the highly active form of clostripain. J Biol Chem. 1979 Mar 10;254(5):1462–1468. [PubMed] [Google Scholar]
  6. Gotschlich E. C., Blake M. S., Koomey J. M., Seiff M., Derman A. Cloning of the structural genes of three H8 antigens and of protein III of Neisseria gonorrhoeae. J Exp Med. 1986 Sep 1;164(3):868–881. doi: 10.1084/jem.164.3.868. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hitchcock P. J., Hayes S. F., Mayer L. W., Shafer W. M., Tessier S. L. Analyses of gonococcal H8 antigen. Surface location, inter- and intrastrain electrophoretic heterogeneity, and unusual two-dimensional electrophoretic characteristics. J Exp Med. 1985 Dec 1;162(6):2017–2034. doi: 10.1084/jem.162.6.2017. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hunkapiller M. W., Hewick R. M., Dreyer W. J., Hood L. E. High-sensitivity sequencing with a gas-phase sequenator. Methods Enzymol. 1983;91:399–413. doi: 10.1016/s0076-6879(83)91038-8. [DOI] [PubMed] [Google Scholar]
  9. Inglis A. S. Cleavage at aspartic acid. Methods Enzymol. 1983;91:324–332. doi: 10.1016/s0076-6879(83)91030-3. [DOI] [PubMed] [Google Scholar]
  10. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  11. Strittmatter W., Hitchcock P. J. Isolation and preliminary biochemical characterization of the gonococcal H.8 antigen. J Exp Med. 1986 Dec 1;164(6):2038–2048. doi: 10.1084/jem.164.6.2038. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Zollinger W. D., Moran E. E., Connelly H., Mandrell R. E., Brandt B. Monoclonal antibodies to serotype 2 and serotype 15 outer membrane proteins of Neisseria meningitidis and their use in serotyping. Infect Immun. 1984 Oct;46(1):260–266. doi: 10.1128/iai.46.1.260-266.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]

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