Abstract
To determine the ultrastructural distribution and immunological accessibility of proteins II (P.IIs) on the surfaces of whole gonococci, anti-P.II gold probes were developed and used in electron microscopic studies of viable P.II-expressing variants of Neisseria gonorrhoeae FA1090. Anti-P.II probes clearly marked the surfaces of organisms and their associated outer membrane blebs. The surface-exposed portion of P.II is antigenically variable. With the use of two different sizes of gold probes, it was demonstrated that individual gonococcal cells can express more than one antigenic type of P.II simultaneously.
Full text
PDF![1003](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bccd/259407/8475747c5597/iai00076-0291.png)
![1004](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bccd/259407/09eca2e08ba5/iai00076-0292.png)
![1005](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bccd/259407/42085c447112/iai00076-0293.png)
![1006](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bccd/259407/3583eddd0979/iai00076-0294.png)
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Barritt D. S., Schwalbe R. S., Klapper D. G., Cannon J. G. Antigenic and structural differences among six proteins II expressed by a single strain of Neisseria gonorrhoeae. Infect Immun. 1987 Sep;55(9):2026–2031. doi: 10.1128/iai.55.9.2026-2031.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Black W. J., Schwalbe R. S., Nachamkin I., Cannon J. G. Characterization of Neisseria gonorrhoeae protein II phase variation by use of monoclonal antibodies. Infect Immun. 1984 Aug;45(2):453–457. doi: 10.1128/iai.45.2.453-457.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Blake M. S., Gotschlich E. C. Gonococcal membrane proteins: speculation on their role in pathogenesis. Prog Allergy. 1983;33:298–313. [PubMed] [Google Scholar]
- Blake M. S., Gotschlich E. C., Swanson J. Effects of proteolytic enzymes on the outer membrane proteins of Neisseria gonorrhoeae. Infect Immun. 1981 Jul;33(1):212–222. doi: 10.1128/iai.33.1.212-222.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Britigan B. E., Cohen M. S., Sparling P. F. Gonococcal infection: a model of molecular pathogenesis. N Engl J Med. 1985 Jun 27;312(26):1683–1694. doi: 10.1056/NEJM198506273122606. [DOI] [PubMed] [Google Scholar]
- Cannon J. G., Black W. J., Nachamkin I., Stewart P. W. Monoclonal antibody that recognizes an outer membrane antigen common to the pathogenic Neisseria species but not to most nonpathogenic Neisseria species. Infect Immun. 1984 Mar;43(3):994–999. doi: 10.1128/iai.43.3.994-999.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Diaz J. L., Heckels J. E. Antigenic variation of outer membrane protein II in colonial variants of Neisseria gonorrhoeae P9. J Gen Microbiol. 1982 Mar;128(3):585–591. doi: 10.1099/00221287-128-3-585. [DOI] [PubMed] [Google Scholar]
- Judd R. C. Structure and surface exposure of protein IIs of Neisseria gonorrhoeae JS3. Infect Immun. 1985 May;48(2):452–457. doi: 10.1128/iai.48.2.452-457.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nachamkin I., Cannon J. G., Mittler R. S. Monoclonal antibodies against Neisseria gonorrhoeae: production of antibodies directed against a strain-specific cell surface antigen. Infect Immun. 1981 May;32(2):641–648. doi: 10.1128/iai.32.2.641-648.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Newhall WJ5th, Mail L. B., Wilde C. E., 3rd, Jones R. B. Purification and antigenic relatedness of proteins II of Neisseria gonorrhoeae. Infect Immun. 1985 Sep;49(3):576–580. doi: 10.1128/iai.49.3.576-580.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Robinson E. N., Jr, McGee Z. A., Buchanan T. M., Blake M. S., Hitchcock P. J. Probing the surface of Neisseria gonorrhoeae: simultaneous localization of protein I and H.8 antigens. Infect Immun. 1987 May;55(5):1190–1197. doi: 10.1128/iai.55.5.1190-1197.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Robinson E. N., Jr, McGee Z. A., Kaplan J., Hammond M. E., Larson J. K., Buchanan T. M., Schoolnik G. K. Ultrastructural localization of specific gonococcal macromolecules with antibody-gold sphere immunological probes. Infect Immun. 1984 Nov;46(2):361–366. doi: 10.1128/iai.46.2.361-366.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schwalbe R. S., Cannon J. G. Genetic transformation of genes for protein II in Neisseria gonorrhoeae. J Bacteriol. 1986 Jul;167(1):186–190. doi: 10.1128/jb.167.1.186-190.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sparling P. F., Cannon J. G., So M. Phase and antigenic variation of pili and outer membrane protein II of Neisseria gonorrhoeae. J Infect Dis. 1986 Feb;153(2):196–201. doi: 10.1093/infdis/153.2.196. [DOI] [PubMed] [Google Scholar]
- Swanson J., Barrera O. Immunological characteristics of gonococcal outer membrane protein II assessed by immunoprecipitation, immunoblotting, and coagglutination. J Exp Med. 1983 May 1;157(5):1405–1420. doi: 10.1084/jem.157.5.1405. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Swanson J. Colony opacity and protein II compositions of gonococci. Infect Immun. 1982 Jul;37(1):359–368. doi: 10.1128/iai.37.1.359-368.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Swanson J. Gonococcal adherence: selected topics. Rev Infect Dis. 1983 Sep-Oct;5 (Suppl 4):S678–S684. doi: 10.1093/clinids/5.supplement_4.s678. [DOI] [PubMed] [Google Scholar]