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. 1988 Jun;56(6):1513–1517. doi: 10.1128/iai.56.6.1513-1517.1988

Molecular analysis of enterotoxin plasmids of enterotoxigenic Escherichia coli of 14 different O serotypes.

H Danbara 1, K Komase 1, H Arita 1, H Abe 1, M Yoshikawa 1
PMCID: PMC259429  PMID: 3286498

Abstract

A total of 104 isolates of enterotoxigenic Escherichia coli derived from diarrheal patients from more than 10 countries were examined for serotype and toxigenicity. The transferability and molecular structure of the enterotoxin plasmids from each isolate were also examined. Enterotoxin plasmids from serotypes such as O6, O25, O27, O126, O128, and O159, which are frequently associated with E. coli diarrhea (classical strains) generally did not transfer by conjugation from clinical isolates, whereas those from serotypes such as O7, O17, O80, O98, O139, O150, and O153, which are rarely associated with diarrhea (rare strains) transferred almost always from the clinical isolates by conjugation. Analyses of enterotoxin plasmids by restriction endonucleases and DNA-DNA hybridization with the enterotoxin probes revealed that the strains with the same O serotype and toxigenicity carry closely related enterotoxin plasmids. These results suggest that classical strains resulted from the dissemination of ancestral clones which received enterotoxin plasmids long ago, while the rare strains acquired the enterotoxin plasmids recently by conjugation and have not yet been spread to the same degree as the ancestral clones.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. DE S. N., BHATTACHARYA K., SARKAR J. K. A study of the pathogenicity of strains of Bacterium coli from acute and chronic enteritis. J Pathol Bacteriol. 1956 Jan;71(1):201–209. doi: 10.1002/path.1700710126. [DOI] [PubMed] [Google Scholar]
  2. Danbara H., Arita H., Baba H., Yoshikawa M. Conjugal acquisition and stable maintenance of Ent plasmids in nontoxigenic wild-type strains of Escherichia coli. Microbiol Immunol. 1986;30(11):1095–1104. doi: 10.1111/j.1348-0421.1986.tb03039.x. [DOI] [PubMed] [Google Scholar]
  3. Danbara H., Komase K., Kirii Y., Shinohara M., Arita H., Makino S., Yoshikawa M. Analysis of the plasmids of Escherichia coli O148:H28 from travellers with diarrhea. Microb Pathog. 1987 Oct;3(4):269–278. doi: 10.1016/0882-4010(87)90060-x. [DOI] [PubMed] [Google Scholar]
  4. Echeverria P., Orskov F., Orskov I., Plianbangchang D. Serotypes of enterotoxigenic Escherichia coli in Thailand and the Philippines. Infect Immun. 1982 Jun;36(3):851–856. doi: 10.1128/iai.36.3.851-856.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Harayama S., Tsuda M., Iino T. High frequency mobilization of the chromosome of Escherichia coli by a mutant of plasmid RP4 temperature-sensitive for maintenance. Mol Gen Genet. 1980;180(1):47–56. doi: 10.1007/BF00267351. [DOI] [PubMed] [Google Scholar]
  6. Honda T., Taga S., Takeda Y., Miwatani T. Modified Elek test for detection of heat-labile enterotoxin of enterotoxigenic Escherichia coli. J Clin Microbiol. 1981 Jan;13(1):1–5. doi: 10.1128/jcm.13.1.1-5.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kado C. I., Liu S. T. Rapid procedure for detection and isolation of large and small plasmids. J Bacteriol. 1981 Mar;145(3):1365–1373. doi: 10.1128/jb.145.3.1365-1373.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kirii Y., Danbara H., Komase K., Arita H., Yoshikawa M. Detection of enterotoxigenic Escherichia coli by colony hybridization with biotinylated enterotoxin probes. J Clin Microbiol. 1987 Oct;25(10):1962–1965. doi: 10.1128/jcm.25.10.1962-1965.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Merson M. H., Orskov F., Orskov I., Sack R. B., Huq I., Koster F. T. Relationship between enterotoxin production and serotype in enterotoxigenic Escherichia coli. Infect Immun. 1979 Feb;23(2):325–329. doi: 10.1128/iai.23.2.325-329.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mundell D. H., Anselmo C. R., Wishnow R. M. Factors influencing heat-labile Escherichia coli enterotoxin activity. Infect Immun. 1976 Aug;14(2):383–388. doi: 10.1128/iai.14.2.383-388.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Orskov F., Orskov I., Evans D. J., Jr, Sack R. B., Sack D. A., Wadström T. Special Escherichia coli serotypes among enterotoxigenic strains from diarrhoea in adults and children. Med Microbiol Immunol. 1976 Jun 1;162(2):73–80. doi: 10.1007/BF02121318. [DOI] [PubMed] [Google Scholar]
  12. Orskov F., Orskov I. From the national institutes of health. Summary of a workshop on the clone concept in the epidemiology, taxonomy, and evolution of the enterobacteriaceae and other bacteria. J Infect Dis. 1983 Aug;148(2):346–357. doi: 10.1093/infdis/148.2.346. [DOI] [PubMed] [Google Scholar]
  13. Reis M. H., Matos D. P., de Castro A. F., Toledo M. R., Trabulsi L. R. Relationship among enterotoxigenic phenotypes, serotypes, and sources of strains in enterotoxigenic Escherichia coli. Infect Immun. 1980 Apr;28(1):24–27. doi: 10.1128/iai.28.1.24-27.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Scotland S. M., Day N. P., Rowe B. Acquisition and maintenance of enterotoxin plasmids in wild-type strains of Escherichia coli. J Gen Microbiol. 1983 Oct;129(10):3111–3120. doi: 10.1099/00221287-129-10-3111. [DOI] [PubMed] [Google Scholar]
  15. Silver R. P., Aaronson W., Sutton A., Schneerson R. Comparative analysis of plasmids and some metabolic characteristics of Escherichia coli K1 from diseased and healthy individuals. Infect Immun. 1980 Jul;29(1):200–206. doi: 10.1128/iai.29.1.200-206.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. So M., Heffron F., McCarthy B. J. The E. coli gene encoding heat stable toxin is a bacterial transposon flanked by inverted repeats of IS1. Nature. 1979 Feb 8;277(5696):453–456. doi: 10.1038/277453a0. [DOI] [PubMed] [Google Scholar]
  17. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  18. Stoll B. J., Rowe B., Glass R. I., Gross R. J., Huq I. Changes in serotypes of enterotoxigenic Escherichia coli in Dhaka over time: usefulness of polyvalent antisera. J Clin Microbiol. 1983 Oct;18(4):935–937. doi: 10.1128/jcm.18.4.935-937.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Takeda Y., Takeda T., Yano T., Yamamoto K., Miwatani T. Purification and partial characterization of heat-stable enterotoxin of enterotoxigenic Escherichia coli. Infect Immun. 1979 Sep;25(3):978–985. doi: 10.1128/iai.25.3.978-985.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

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