Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1988 Jun;56(6):1652–1654. doi: 10.1128/iai.56.6.1652-1654.1988

Acid phosphatase stimulation of the growth of Nocardia asteroides and its possible relationship to the modification of lysosomal enzymes in macrophages.

L Beaman 1, M Paliescheskey 1, B L Beaman 1
PMCID: PMC259451  PMID: 3286504

Abstract

Lysosomal acid phosphatase levels are reduced in murine macrophages by virulent strains of Nocardia asteroides. At the same time, other lysosomal enzymes either remain unchanged or increase in activity, indicating that acid phosphatase is not lost because of degranulation or membrane leakage. This study shows that acid phosphatase was utilized as a sole carbon source by Nocardia asteroides and that acid phosphatase combined with glutamate as a carbon source enhanced nocardial growth. As a consequence, the inverse relationship that was observed between acid phosphatase activity and the bactericidal capacity of macrophages infected with N. asteroides appears to be due to the ability of N. asteroides to preferentially metabolize this lysosomal enzyme during growth within phagocytes.

Full text

PDF
1652

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beaman B. L., Gershwin M. E., Ahmed A., Scates S. M., Deem R. Response of CBA/N x DBA2/F1 mice to Nocardia asteroides. Infect Immun. 1982 Jan;35(1):111–116. doi: 10.1128/iai.35.1.111-116.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Beaman B. L., Gershwin M. E., Scates S. S., Ohsugi Y. Immunobiology of germfree mice infected with Nocardia asteroides. Infect Immun. 1980 Aug;29(2):733–743. doi: 10.1128/iai.29.2.733-743.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Beaman B. L., Goldstein E., Gershwin M. E., Maslan S., Lippert W. Lung response to congenitally athymic (nude), heterozygous, and Swiss Webster mice to aerogenic and intranasal infection by Nocardia asteroides. Infect Immun. 1978 Dec;22(3):867–877. doi: 10.1128/iai.22.3.867-877.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Beaman B. L., Maslan S. Virulence of Nocardia asteroides during its growth cycle. Infect Immun. 1978 Apr;20(1):290–295. doi: 10.1128/iai.20.1.290-295.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Beaman B. L., Smathers M. Interaction of Nocardia asteroides with cultured rabbit alveolar macrophages. Infect Immun. 1976 Apr;13(4):1126–1131. doi: 10.1128/iai.13.4.1126-1131.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Black C. M., Beaman B. L., Donovan R. M., Goldstein E. Effect of virulent and less virulent strains of Nocardia asteroides on acid-phosphatase activity in alveolar and peritoneal macrophages maintained in vitro. J Infect Dis. 1983 Jul;148(1):117–124. doi: 10.1093/infdis/148.1.117. [DOI] [PubMed] [Google Scholar]
  7. Black C. M., Beaman B. L., Donovan R. M., Goldstein E. Intracellular acid phosphatase content and ability of different macrophage populations to kill Nocardia asteroides. Infect Immun. 1985 Feb;47(2):375–383. doi: 10.1128/iai.47.2.375-383.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Black C. M., Paliescheskey M., Beaman B. L., Donovan R. M., Goldstein E. Acidification of phagosomes in murine macrophages: blockage by Nocardia asteroides. J Infect Dis. 1986 Dec;154(6):952–958. doi: 10.1093/infdis/154.6.952. [DOI] [PubMed] [Google Scholar]
  9. Black C. M., Paliescheskey M., Beaman B. L., Donovan R. M., Goldstein E. Modulation of lysosomal protease-esterase and lysozyme in Kupffer cells and peritoneal macrophages infected with Nocardia asteroides. Infect Immun. 1986 Dec;54(3):917–919. doi: 10.1128/iai.54.3.917-919.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Davis-Scibienski C., Beaman B. L. Interaction of Nocardia asteroides with rabbit alveolar macrophages: association of virulence, viability, ultrastructural damage, and phagosome-lysosome fusion. Infect Immun. 1980 May;28(2):610–619. doi: 10.1128/iai.28.2.610-619.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Filice G. A., Beaman B. L., Krick J. A., Remington J. S. Effects of human neutrophils and monocytes on Nocardia asteroides: failure of killing despite occurrence of the oxidative metabolic burst. J Infect Dis. 1980 Sep;142(3):432–438. doi: 10.1093/infdis/142.3.432. [DOI] [PubMed] [Google Scholar]
  12. GORDON R. E., MIHM J. M. A comparative study of some strains received as nocardiae. J Bacteriol. 1957 Jan;73(1):15–27. doi: 10.1128/jb.73.1.15-27.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Gordon R. E. Some strains in search of a genus--Corynebacterium, Mycobacterium, Nocardia or what? J Gen Microbiol. 1966 Jun;43(3):329–343. doi: 10.1099/00221287-43-3-329. [DOI] [PubMed] [Google Scholar]
  14. Stanier R. Y., Palleroni N. J., Doudoroff M. The aerobic pseudomonads: a taxonomic study. J Gen Microbiol. 1966 May;43(2):159–271. doi: 10.1099/00221287-43-2-159. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES