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. 1988 Jun;56(6):1658–1660. doi: 10.1128/iai.56.6.1658-1660.1988

Identification of Babesia bigemina and Babesia bovis merozoite proteins with isolate- and species-common epitopes recognized by antibodies in bovine immune sera.

T F McElwain 1, G H Palmer 1, W L Goff 1, T C McGuire 1
PMCID: PMC259453  PMID: 2453470

Abstract

Immunoprecipitation of radiolabeled antigens with bovine antisera indicated that many Babesia bigemina and Babesia bovis merozoite proteins contain isolate-common epitopes, while at least 16 B. bigemina and 8 B. bovis proteins contain species-cross-reactive epitopes. One immunogenic, isolate-common, and species-specific candidate diagnostic protein from each species was identified.

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Selected References

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  1. Callow L. L. Vaccination against bovine babesiosis. Adv Exp Med Biol. 1977;93:121–149. doi: 10.1007/978-1-4615-8855-9_9. [DOI] [PubMed] [Google Scholar]
  2. Cowman A. F., Timms P., Kemp D. J. DNA polymorphisms and subpopulations in Babesia bovis. Mol Biochem Parasitol. 1984 Apr;11:91–103. doi: 10.1016/0166-6851(84)90057-4. [DOI] [PubMed] [Google Scholar]
  3. Dalgliesh R. J., Callow L. L., Mellors L. T., McGregor W. Development of a highly infective Babesia bigemina vaccine of reduced virulence. Aust Vet J. 1981 Jan;57(1):8–11. doi: 10.1111/j.1751-0813.1981.tb07075.x. [DOI] [PubMed] [Google Scholar]
  4. De Vos A. J., Combrink M. P., Bessenger R. Babesia bigemina vaccine: comparison of the efficacy and safety of Australian and South African strains under experimental conditions in South Africa. Onderstepoort J Vet Res. 1982 Sep;49(3):155–158. [PubMed] [Google Scholar]
  5. Goff W. L., Yunker C. E. Babesia bovis: increased percentage parasitized erythrocytes in cultures and assessment of growth by incorporation of [3H]hypoxanthine. Exp Parasitol. 1986 Oct;62(2):202–210. doi: 10.1016/0014-4894(86)90024-x. [DOI] [PubMed] [Google Scholar]
  6. Goodger B. V., Commins M. A., Wright I. G., Mirre G. B. Babesia bovis: vaccination of cattle against heterologous challenge with fractions of lysate from infected erythrocytes. Z Parasitenkd. 1984;70(3):321–329. doi: 10.1007/BF00927818. [DOI] [PubMed] [Google Scholar]
  7. Goodger B. V. Further studies of haemagglutinating antigens of Babesia bigemina. Aust Vet J. 1973 Feb;49(2):81–84. doi: 10.1111/j.1751-0813.1973.tb09321.x. [DOI] [PubMed] [Google Scholar]
  8. Goodger B. V. Preparation and preliminary assessment of purified antigens in the passive haemagglutination test for bovine babesiosis. Aust Vet J. 1971 Jun;47(6):251–256. doi: 10.1111/j.1751-0813.1971.tb02144.x. [DOI] [PubMed] [Google Scholar]
  9. Kahl L. P., Anders R. F., Rodwell B. J., Timms P., Mitchell G. F. Variable and common antigens of Babesia bovis parasites differing in strain and virulence. J Immunol. 1982 Oct;129(4):1700–1705. [PubMed] [Google Scholar]
  10. Leeflang P., Perié N. M. Comparative immunofluorescent studies on 4 Babesia species of cattle. Res Vet Sci. 1972 Jul;13(4):342–346. [PubMed] [Google Scholar]
  11. Levy M. G., Ristic M. Babesia bovis: continuous cultivation in a microaerophilous stationary phase culture. Science. 1980 Mar 14;207(4436):1218–1220. doi: 10.1126/science.7355284. [DOI] [PubMed] [Google Scholar]
  12. Love J. N. Cryogenic preservation of Anaplasma marginale with Dimethyl sulfoxide. Am J Vet Res. 1972 Dec;33(12):2557–2560. [PubMed] [Google Scholar]
  13. Mahoney D. F. Bovine babesiosis: preparation and assessment of complement fixing antigens. Exp Parasitol. 1967 Apr;20(2):232–241. doi: 10.1016/0014-4894(67)90043-4. [DOI] [PubMed] [Google Scholar]
  14. Mahoney D. F., Wright I. G., Goodger B. V. Bovine babesiosis: the immunization of cattle with fractions of erythrocytes infected with Babesia bovis (syn B. argentina). Vet Immunol Immunopathol. 1981 Apr;2(2):145–156. doi: 10.1016/0165-2427(81)90046-5. [DOI] [PubMed] [Google Scholar]
  15. McElwain T. F., Perryman L. E., Davis W. C., McGuire T. C. Antibodies define multiple proteins with epitopes exposed on the surface of live Babesia bigemina merozoites. J Immunol. 1987 Apr 1;138(7):2298–2304. [PubMed] [Google Scholar]
  16. Montenegro-James S., Ristic M., Toro Benitez M., Leon E., Lopez R. Heterologous strain immunity in bovine babesiosis using a culture-derived soluble Babesia bovis immunogen. Vet Parasitol. 1985 Dec;18(4):321–337. doi: 10.1016/0304-4017(85)90067-6. [DOI] [PubMed] [Google Scholar]
  17. Smith R. D., Molinar E., Larios F., Monroy J., Trigo F., Ristic M. Bovine babesiosis: pathogenicity and heterologous species immunity of tick-borne Babesia bovis and B bigemina infections. Am J Vet Res. 1980 Dec;41(12):1957–1965. [PubMed] [Google Scholar]
  18. Timms P., Dalgliesh R. J., Barry D. N., Dimmock C. K., Rodwell B. J. Babesia bovis: comparison of culture-derived parasites, non-living antigen and conventional vaccine in the protection of cattle against heterologous challenge. Aust Vet J. 1983 Mar;60(3):75–77. doi: 10.1111/j.1751-0813.1983.tb05874.x. [DOI] [PubMed] [Google Scholar]
  19. Wright I. G., Goodger B. V., Leatch G., Aylward J. H., Rode-Bramanis K., Waltisbuhl D. J. Protection of Babesia bigemina-immune animals against subsequent challenge with virulent Babesia bovis. Infect Immun. 1987 Feb;55(2):364–368. doi: 10.1128/iai.55.2.364-368.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]

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