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. 1988 Jun;56(6):1665–1667. doi: 10.1128/iai.56.6.1665-1667.1988

Cloning of the gene, speB, for streptococcal pyrogenic exotoxin type B in Escherichia coli.

G A Bohach 1, A R Hauser 1, P M Schlievert 1
PMCID: PMC259455  PMID: 3286506

Abstract

The structural gene encoding streptococcal pyrogenic exotoxin type B, designated speB, was cloned in Escherichia coli and localized onto a 4.5-kilobase BamHI-BglII DNA fragment. Streptococcal pyrogenic exotoxin type B, partially purified from E. coli clones, was immunologically related to streptococcus-derived toxin. Also, toxin derived from either E. coli or Streptococcus pyogenes had similar lymphocyte mitogenic activity and molecular weight (29,300) and displayed comparable microheterogeneity when evaluated by isoelectric focusing.

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Selected References

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