Skip to main content
Infection and Immunity logoLink to Infection and Immunity
. 1988 Aug;56(8):1956–1960. doi: 10.1128/iai.56.8.1956-1960.1988

Sequence analysis of the Streptococcus mutans scrB gene.

Y Sato 1, H K Kuramitsu 1
PMCID: PMC259507  PMID: 3397182

Abstract

The complete nucleotide sequence of the Streptococcus mutans GS-5 scrB gene coding for sucrose-6-phosphate hydrolase activity was determined. A potential ribosome-binding site as well as promoter sequences were identified upstream from the gene. The deduced amino acid sequence of the enzyme suggested a molecular weight of 51,750, which is similar to that estimated for the enzyme isolated from strain GS-5. The enzyme is slightly acidic, with a pI of 5.9, and is a relatively hydrophilic protein. The nucleotide and amino acid sequences of the enzyme showed significant homology with those of the sacA protein from Bacillus subtilis. In addition, a region of amino acid homology with the S. mutans fructosyltransferase and B. subtilis levansucrase proteins was also detected.

Full text

PDF
1956

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aoki H., Shiroza T., Hayakawa M., Sato S., Kuramitsu H. K. Cloning of a Streptococcus mutans glucosyltransferase gene coding for insoluble glucan synthesis. Infect Immun. 1986 Sep;53(3):587–594. doi: 10.1128/iai.53.3.587-594.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bozzola J. J., Kuramitsu H. K., Maynard M. T. Localization of Streptococcus mutans GS-5 glucosyltransferases and intracellular invertase. Infect Immun. 1981 May;32(2):830–839. doi: 10.1128/iai.32.2.830-839.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Breidt F., Jr, Hengstenberg W., Finkeldei U., Stewart G. C. Identification of the genes for the lactose-specific components of the phosphotransferase system in the lac operon of Staphylococcus aureus. J Biol Chem. 1987 Dec 5;262(34):16444–16449. [PubMed] [Google Scholar]
  4. Chassy B. M., Porter E. V. Initial characterization of sucrose-6-phosphate hydrolase from Streptococcus mutans and its apparent identity with intracellular invertase. Biochem Biophys Res Commun. 1979 Jul 12;89(1):307–314. doi: 10.1016/0006-291x(79)90979-3. [DOI] [PubMed] [Google Scholar]
  5. Coykendall A. L. Four types of Streptococcus mutans based on their genetic, antigenic and biochemical characteristics. J Gen Microbiol. 1974 Aug;83(2):327–338. doi: 10.1099/00221287-83-2-327. [DOI] [PubMed] [Google Scholar]
  6. Fahnestock S. R., Alexander P., Nagle J., Filpula D. Gene for an immunoglobulin-binding protein from a group G streptococcus. J Bacteriol. 1986 Sep;167(3):870–880. doi: 10.1128/jb.167.3.870-880.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fouet A., Klier A., Rapoport G. Nucleotide sequence of the sucrase gene of Bacillus subtilis. Gene. 1986;45(2):221–225. doi: 10.1016/0378-1119(86)90258-1. [DOI] [PubMed] [Google Scholar]
  8. Hayakawa M., Aoki H., Kuramitsu H. K. Isolation and characterization of the sucrose 6-phosphate hydrolase gene from Streptococcus mutans. Infect Immun. 1986 Sep;53(3):582–586. doi: 10.1128/iai.53.3.582-586.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hollingshead S. K., Fischetti V. A., Scott J. R. Complete nucleotide sequence of type 6 M protein of the group A Streptococcus. Repetitive structure and membrane anchor. J Biol Chem. 1986 Feb 5;261(4):1677–1686. [PubMed] [Google Scholar]
  10. Kuramitsu H. K. Characterization of invertase activity from cariogenic Streptococcus mutans. J Bacteriol. 1973 Sep;115(3):1003–1010. doi: 10.1128/jb.115.3.1003-1010.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kuramitsu H. K. Utilization of a mini-mu transposon to construct defined mutants in Streptococcus mutans. Mol Microbiol. 1987 Sep;1(2):229–232. doi: 10.1111/j.1365-2958.1987.tb00516.x. [DOI] [PubMed] [Google Scholar]
  12. Kyte J., Doolittle R. F. A simple method for displaying the hydropathic character of a protein. J Mol Biol. 1982 May 5;157(1):105–132. doi: 10.1016/0022-2836(82)90515-0. [DOI] [PubMed] [Google Scholar]
  13. Loesche W. J. Role of Streptococcus mutans in human dental decay. Microbiol Rev. 1986 Dec;50(4):353–380. doi: 10.1128/mr.50.4.353-380.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lunsford R. D., Macrina F. L. Molecular cloning and characterization of scrB, the structural gene for the Streptococcus mutans phosphoenolpyruvate-dependent sucrose phosphotransferase system sucrose-6-phosphate hydrolase. J Bacteriol. 1986 May;166(2):426–434. doi: 10.1128/jb.166.2.426-434.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Maynard M. T., Kuramitsu H. K. Purification and antigenic properties of intracellular invertase from Streptococcus mutans. Infect Immun. 1979 Mar;23(3):873–883. doi: 10.1128/iai.23.3.873-883.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Shaw J. H., Clewell D. B. Complete nucleotide sequence of macrolide-lincosamide-streptogramin B-resistance transposon Tn917 in Streptococcus faecalis. J Bacteriol. 1985 Nov;164(2):782–796. doi: 10.1128/jb.164.2.782-796.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Shiroza T., Kuramitsu H. K. Sequence analysis of the Streptococcus mutans fructosyltransferase gene and flanking regions. J Bacteriol. 1988 Feb;170(2):810–816. doi: 10.1128/jb.170.2.810-816.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Shiroza T., Ueda S., Kuramitsu H. K. Sequence analysis of the gtfB gene from Streptococcus mutans. J Bacteriol. 1987 Sep;169(9):4263–4270. doi: 10.1128/jb.169.9.4263-4270.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Slee A. M., Tanzer J. M. Sucrose transport by Streptococcus mutans. Evidence for multiple transport systems. Biochim Biophys Acta. 1982 Nov 22;692(3):415–424. doi: 10.1016/0005-2736(82)90392-3. [DOI] [PubMed] [Google Scholar]
  21. St Martin E. J., Wittenberger C. L. Characterization of a phosphoenolpyruvate-dependent sucrose phosphotransferase system in Streptococcus mutans. Infect Immun. 1979 Jun;24(3):865–868. doi: 10.1128/iai.24.3.865-868.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Steinmetz M., Le Coq D., Aymerich S., Gonzy-Tréboul G., Gay P. The DNA sequence of the gene for the secreted Bacillus subtilis enzyme levansucrase and its genetic control sites. Mol Gen Genet. 1985;200(2):220–228. doi: 10.1007/BF00425427. [DOI] [PubMed] [Google Scholar]
  23. Tanzer J. M., Brown A. T., McInerney M. F. Identification, preliminary characterization, and evidence for regulation of invertase in Streptococcus mutans. J Bacteriol. 1973 Oct;116(1):192–202. doi: 10.1128/jb.116.1.192-202.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Tanzer J. M., Chassy B. M., Krichevsky M. I. Sucrose metabolism by Streptococcus mutans, SL-I. Biochim Biophys Acta. 1971 Feb 28;261(2):379–387. doi: 10.1016/0304-4165(72)90062-1. [DOI] [PubMed] [Google Scholar]
  25. Taussig R., Carlson M. Nucleotide sequence of the yeast SUC2 gene for invertase. Nucleic Acids Res. 1983 Mar 25;11(6):1943–1954. doi: 10.1093/nar/11.6.1943. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES