Abstract
We have identified and cloned a 6-kilobase-pair segment of chromosomal DNA from Streptococcus sanguis ATCC 10556 that encodes immunoglobulin A (IgA) protease activity when cloned into Escherichia coli. The enzyme specified by the iga gene in plasmid pJG1 accumulates in the periplasm of E. coli MM294 cells and has a substrate specificity for human IgA1 identical to that of native S. sanguis protease. Hybridization experiments with probes from within the encoding DNA showed no detectable homology at the nucleotide sequence level with chromosomal DNA of gram-negative bacteria that excrete IgA protease. Moreover, the S. sanguis iga gene probes showed no detectable hybridization with chromosomal DNA of S. pneumoniae, although the IgA proteases of these two streptococcal species cleaved the identical peptide bond in the human IgA1 heavy-chain hinge region.
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- Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bricker J., Mulks M. H., Plaut A. G., Moxon E. R., Wright A. IgA1 proteases of Haemophilus influenzae: cloning and characterization in Escherichia coli K-12. Proc Natl Acad Sci U S A. 1983 May;80(9):2681–2685. doi: 10.1073/pnas.80.9.2681. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cooper M. D., McGee Z. A., Mulks M. H., Koomey J. M., Hindman T. L. Attachment to and invasion of human fallopian tube mucosa by an IgA1 protease-deficient mutant of Neisseria gonorrhoeae and its wild-type parent. J Infect Dis. 1984 Nov;150(5):737–744. doi: 10.1093/infdis/150.5.737. [DOI] [PubMed] [Google Scholar]
- Coykendall A. L. Base composition of deoxyribonucleic acid isolated from cariogenic streptococci. Arch Oral Biol. 1970 Apr;15(4):365–368. doi: 10.1016/0003-9969(70)90063-4. [DOI] [PubMed] [Google Scholar]
- Enquist L., Sternberg N. In vitro packaging of lambda Dam vectors and their use in cloning DNA fragments. Methods Enzymol. 1979;68:281–298. doi: 10.1016/0076-6879(79)68020-5. [DOI] [PubMed] [Google Scholar]
- Facklam R. R. Physiological differentiation of viridans streptococci. J Clin Microbiol. 1977 Feb;5(2):184–201. doi: 10.1128/jcm.5.2.184-201.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Farley M. M., Stephens D. S., Mulks M. H., Cooper M. D., Bricker J. V., Mirra S. S., Wright A. Pathogenesis of IgA1 protease-producing and -nonproducing Haemophilus influenzae in human nasopharyngeal organ cultures. J Infect Dis. 1986 Nov;154(5):752–759. doi: 10.1093/infdis/154.5.752. [DOI] [PubMed] [Google Scholar]
- Frandsen E. V., Reinholdt J., Kilian M. Enzymatic and antigenic characterization of immunoglobulin A1 proteases from Bacteroides and Capnocytophaga spp. Infect Immun. 1987 Mar;55(3):631–638. doi: 10.1128/iai.55.3.631-638.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gilbert J. V., Plaut A. G. Detection of IgA protease activity among multiple bacterial colonies. J Immunol Methods. 1983 Feb 25;57(1-3):247–251. doi: 10.1016/0022-1759(83)90084-4. [DOI] [PubMed] [Google Scholar]
- Gilbert J. V., Plaut A. G., Longmaid B., Lamm M. E. Inhibition of microbial IgA proteases by human secretory IgA and serum. Mol Immunol. 1983 Sep;20(9):1039–1049. doi: 10.1016/0161-5890(83)90045-7. [DOI] [PubMed] [Google Scholar]
- Gutierrez C., Barondess J., Manoil C., Beckwith J. The use of transposon TnphoA to detect genes for cell envelope proteins subject to a common regulatory stimulus. Analysis of osmotically regulated genes in Escherichia coli. J Mol Biol. 1987 May 20;195(2):289–297. doi: 10.1016/0022-2836(87)90650-4. [DOI] [PubMed] [Google Scholar]
- Jack G. W., Richmond M. H. A comparative study of eight distinct beta-lactamases synthesized by gram-negative bacteria. J Gen Microbiol. 1970 Apr;61(1):43–61. doi: 10.1099/00221287-61-1-43. [DOI] [PubMed] [Google Scholar]
- Kilian M., Holmgren K. Ecology and nature of immunoglobulin A1 protease-producing streptococci in the human oral cavity and pharynx. Infect Immun. 1981 Mar;31(3):868–873. doi: 10.1128/iai.31.3.868-873.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Koomey J. M., Falkow S. Nucleotide sequence homology between the immunoglobulin A1 protease genes of Neisseria gonorrhoeae, Neisseria meningitidis, and Haemophilus influenzae. Infect Immun. 1984 Jan;43(1):101–107. doi: 10.1128/iai.43.1.101-107.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Koomey J. M., Gill R. E., Falkow S. Genetic and biochemical analysis of gonococcal IgA1 protease: cloning in Escherichia coli and construction of mutants of gonococci that fail to produce the activity. Proc Natl Acad Sci U S A. 1982 Dec;79(24):7881–7885. doi: 10.1073/pnas.79.24.7881. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Loesche W. J. Role of Streptococcus mutans in human dental decay. Microbiol Rev. 1986 Dec;50(4):353–380. doi: 10.1128/mr.50.4.353-380.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Manoil C., Beckwith J. TnphoA: a transposon probe for protein export signals. Proc Natl Acad Sci U S A. 1985 Dec;82(23):8129–8133. doi: 10.1073/pnas.82.23.8129. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mortensen S. B., Kilian M. Purification and characterization of an immunoglobulin A1 protease from Bacteroides melaninogenicus. Infect Immun. 1984 Sep;45(3):550–557. doi: 10.1128/iai.45.3.550-557.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mulks M. H., Kornfeld S. J., Plaut A. G. Specific proteolysis of human IgA by Streptococcus pneumoniae and Haemophilus influenzae. J Infect Dis. 1980 Apr;141(4):450–456. doi: 10.1093/infdis/141.4.450. [DOI] [PubMed] [Google Scholar]
- Neu H. C., Heppel L. A. The release of enzymes from Escherichia coli by osmotic shock and during the formation of spheroplasts. J Biol Chem. 1965 Sep;240(9):3685–3692. [PubMed] [Google Scholar]
- Perry D., Nilsen L. J., Kuramitsu H. K. Mapping of a cloned glucosyltransferase gene in Streptococcus mutans. Infect Immun. 1985 Oct;50(1):130–135. doi: 10.1128/iai.50.1.130-135.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Plaut A. G., Gilbert J. V., Leger G., Blumenstein M. IgA1 protease cleaves heavy chains independently in dimeric human IgA1. Mol Immunol. 1985 Jul;22(7):821–826. doi: 10.1016/0161-5890(85)90148-8. [DOI] [PubMed] [Google Scholar]
- Plaut A. G. The IgA1 proteases of pathogenic bacteria. Annu Rev Microbiol. 1983;37:603–622. doi: 10.1146/annurev.mi.37.100183.003131. [DOI] [PubMed] [Google Scholar]
- Pohlner J., Halter R., Beyreuther K., Meyer T. F. Gene structure and extracellular secretion of Neisseria gonorrhoeae IgA protease. 1987 Jan 29-Feb 4Nature. 325(6103):458–462. doi: 10.1038/325458a0. [DOI] [PubMed] [Google Scholar]
- Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
- Sancar A., Hack A. M., Rupp W. D. Simple method for identification of plasmid-coded proteins. J Bacteriol. 1979 Jan;137(1):692–693. doi: 10.1128/jb.137.1.692-693.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Saunders C. W., Guild W. R. Properties and transforming activities of two plasmids in Streptococcus pneumoniae. Mol Gen Genet. 1980;180(3):573–578. doi: 10.1007/BF00268062. [DOI] [PubMed] [Google Scholar]
- Senda S., Fujiyama Y., Ushijima T., Hodohara K., Bamba T., Hosoda S., Kobayashi K. Clostridium ramosum, an IgA protease-producing species and its ecology in the human intestinal tract. Microbiol Immunol. 1985;29(11):1019–1028. doi: 10.1111/j.1348-0421.1985.tb00892.x. [DOI] [PubMed] [Google Scholar]
- Shaw J. H. Causes and control of dental caries. N Engl J Med. 1987 Oct 15;317(16):996–1004. doi: 10.1056/NEJM198710153171605. [DOI] [PubMed] [Google Scholar]
- Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
- Tsuzukida Y., Wang C. C., Putnam F. W. Structure of the A2m(1) allotype of human IgA--a recombinant molecule. Proc Natl Acad Sci U S A. 1979 Mar;76(3):1104–1108. doi: 10.1073/pnas.76.3.1104. [DOI] [PMC free article] [PubMed] [Google Scholar]