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Infection and Immunity logoLink to Infection and Immunity
. 1988 Aug;56(8):1999–2005. doi: 10.1128/iai.56.8.1999-2005.1988

Isolation and characterization of the Streptococcus mutans gtfC gene, coding for synthesis of both soluble and insoluble glucans.

N Hanada 1, H K Kuramitsu 1
PMCID: PMC259514  PMID: 2969375

Abstract

The intact gtfC gene from Streptococcus mutans GS-5 was isolated in Escherichia coli in plasmid vector pUC18. The glucosyltransferase activity expressed by the gene synthesized both low-molecular-weight water-soluble glucan and insoluble glucan in a primer-independent manner. Purification of the enzyme by procedures that minimize proteolytic digestion yielded a purified preparation with a molecular weight of 140,000. Insertional inactivation of the gtfC gene with a streptococcal erythromycin resistance gene fragment followed by transformation of strain GS-5 suggested that the gtfC gene product was required for sucrose-dependent colonization in vitro. In addition, evidence for the presence of a third gtf gene coding for soluble glucan synthesis was obtained following the construction of mutants containing deletions of both the gtfB and gtfC genes.

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Selected References

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  1. Aoki H., Shiroza T., Hayakawa M., Sato S., Kuramitsu H. K. Cloning of a Streptococcus mutans glucosyltransferase gene coding for insoluble glucan synthesis. Infect Immun. 1986 Sep;53(3):587–594. doi: 10.1128/iai.53.3.587-594.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bolivar F., Backman K. Plasmids of Escherichia coli as cloning vectors. Methods Enzymol. 1979;68:245–267. doi: 10.1016/0076-6879(79)68018-7. [DOI] [PubMed] [Google Scholar]
  3. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  4. Coleman K. J., Cornish-Bowden A., Cole J. A. Purification and properties of nitrite reductase from Escherichia coli K12. Biochem J. 1978 Nov 1;175(2):483–493. doi: 10.1042/bj1750483. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ferretti J. J., Gilpin M. L., Russell R. R. Nucleotide sequence of a glucosyltransferase gene from Streptococcus sobrinus MFe28. J Bacteriol. 1987 Sep;169(9):4271–4278. doi: 10.1128/jb.169.9.4271-4278.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gilpin M. L., Russell R. R., Morrissey P. Cloning and expression of two Streptococcus mutans glucosyltransferases in Escherichia coli K-12. Infect Immun. 1985 Aug;49(2):414–416. doi: 10.1128/iai.49.2.414-416.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kuramitsu H. K. Characterization of extracellular glucosyltransferase activity of Steptococcus mutans. Infect Immun. 1975 Oct;12(4):738–749. doi: 10.1128/iai.12.4.738-749.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kuramitsu H. K., Wondrack L. Insoluble glucan synthesis by Streptococcus mutans serotype c strains. Infect Immun. 1983 Nov;42(2):763–770. doi: 10.1128/iai.42.2.763-770.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Loesche W. J. Role of Streptococcus mutans in human dental decay. Microbiol Rev. 1986 Dec;50(4):353–380. doi: 10.1128/mr.50.4.353-380.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mukasa H., Shimamura A., Tsumori H. Purification and characterization of basic glucosyltransferase from Streptococcus mutans serotype c. Biochim Biophys Acta. 1982 Oct 28;719(1):81–89. doi: 10.1016/0304-4165(82)90310-5. [DOI] [PubMed] [Google Scholar]
  11. Mukasa H., Tsumori H., Shimamura A. Isolation and characterization of an extracellular glucosyltransferase synthesizing insoluble glucan from Streptococcus mutans serotype c. Infect Immun. 1985 Sep;49(3):790–796. doi: 10.1128/iai.49.3.790-796.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Newman B. M., White P., Mohan S. B., Cole J. A. Effect of dextran and ammonium sulphate on the reaction catalysed by a glucosyltransferase complex from Streptococcus mutans. J Gen Microbiol. 1980 Jun;118(2):353–366. doi: 10.1099/00221287-118-2-353. [DOI] [PubMed] [Google Scholar]
  13. Olson G. A., Bleiweis A. S., Small P. A., Jr Adherence inhibition of Streptococcus mutans: an assay reflecting a possible role of antibody in dental caries prophylaxis. Infect Immun. 1972 Apr;5(4):419–427. doi: 10.1128/iai.5.4.419-427.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Perry D., Wondrack L. M., Kuramitsu H. K. Genetic transformation of putative cariogenic properties in Streptococcus mutans. Infect Immun. 1983 Aug;41(2):722–727. doi: 10.1128/iai.41.2.722-727.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pucci M. J., Jones K. R., Kuramitsu H. K., Macrina F. L. Molecular cloning and characterization of the glucosyltransferase C gene (gtfC) from Streptococcus mutans LM7. Infect Immun. 1987 Sep;55(9):2176–2182. doi: 10.1128/iai.55.9.2176-2182.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sato S., Kuramitsu H. K. Isolation and characterization of a fructosyltransferase gene from Streptococcus mutans GS-5. Infect Immun. 1986 Apr;52(1):166–170. doi: 10.1128/iai.52.1.166-170.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Shimamura A., Tsumori H., Mukasa H. Three kinds of extracellular glucosyltransferases from Streptococcus mutans 6715 (serotype g). FEBS Lett. 1983 Jun 27;157(1):79–84. doi: 10.1016/0014-5793(83)81120-x. [DOI] [PubMed] [Google Scholar]
  18. Shiroza T., Ueda S., Kuramitsu H. K. Sequence analysis of the gtfB gene from Streptococcus mutans. J Bacteriol. 1987 Sep;169(9):4263–4270. doi: 10.1128/jb.169.9.4263-4270.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Ueda S., Kuramitsu H. K. Molecular basis for the spontaneous generation of colonization-defective mutants of Streptococcus mutans. Mol Microbiol. 1988 Jan;2(1):135–140. doi: 10.1111/j.1365-2958.1988.tb00014.x. [DOI] [PubMed] [Google Scholar]
  20. Yanisch-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103–119. doi: 10.1016/0378-1119(85)90120-9. [DOI] [PubMed] [Google Scholar]

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