Skip to main content
NCBI home page
The Yale Journal of Biology and Medicine logoLink to The Yale Journal of Biology and Medicine
. 1975 Jul;48(3):217–228.

The multiple mixed lymphocyte reaction: variables important in the test as a measure of lymphocyte competence in man.

R J Mangi, F S Kantor
PMCID: PMC2595221  PMID: 51541

Abstract

In order to utilize the mixed lymphocyte reaction (MLR) as an assay of T-lymphocyte competence, pools of target lymphocytes obtained from different individuals are used to increase the magnitude and decrease the variation of the in vitro response. We evaluated variations in MLR response due to variations in target cell populations. Response increased with an increased target/responder cell ratio. Peak response occurred with a target/responder cell ratio of between 1:1 and 1:4. Response to a pool of lymphocytes from different individuals increased as the number of individuals contributing to the pool increased. Peak stimulation occurred with three to four different donors to the target cell pool. Stimulation produced by pooled target cells resulted in a higher mean index of stimulation and decreased variation of response as compared to stimulation produced by target cells from individual donors. Stimulation produced by pooled target cells was approximately equal to the sum of the stimulation produced by each of the target cell populations acting alone. These findings indicate a practical method of modifying the MLR as a test of T-lymphocyte function.

Full text

PDF
217

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Albertini R. J., Bach F. H. Quantitative assay of antigenic disparity at hl-a-the major histocompatibility locus in man. J Exp Med. 1968 Oct 1;128(4):639–651. doi: 10.1084/jem.128.4.639. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bach F. H., Kisken W. A. Predictive value of results of mixed leukocyte cultures for skin allograft survival in man. Transplantation. 1967 Jul;5(4 Suppl):1046–1052. doi: 10.1097/00007890-196707001-00039. [DOI] [PubMed] [Google Scholar]
  3. Bach F. H., Segall M. Genetics of the mixed leukocyte culture response: a reexamination. Transplant Proc. 1972 Jun;4(2):205–208. [PubMed] [Google Scholar]
  4. Bach J. F., Debray-Sachs M., Crosnier J., Kreis H., Dormont J. Correlation between mixed lymphocyte culture performed before renal transplantation and kidney function. Clin Exp Immunol. 1970 Jun;6(6):821–827. [PMC free article] [PubMed] [Google Scholar]
  5. Colley D. G., DeWitt C. W. Mixed lymphocyte blastogenesis in response to multiple histocompatibility antigens. J Immunol. 1969 Jan;102(1):107–116. [PubMed] [Google Scholar]
  6. Dwyer J. M., Kantor F. S. Regulation of delayed hypersensitivity. Failure to transfer delayed hypersensitivity to desensitized guinea pigs. J Exp Med. 1973 Jan 1;137(1):32–41. doi: 10.1084/jem.137.1.32. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gordon J. Role of monocytes in the mixed leukocyte culture reaction. Proc Soc Exp Biol Med. 1968 Jan;127(1):30–33. doi: 10.3181/00379727-127-32613. [DOI] [PubMed] [Google Scholar]
  8. Han T., Pauly J. Synergism of stimulating capacity of pooled lymphocytes in the mixed lymphocyte reaction. Immunology. 1973 May;24(5):895–899. [PMC free article] [PubMed] [Google Scholar]
  9. Kasakura S., Lowenstein L. A factor stimulating DNA synthesis derived from the medium of leukocyte cultures. Nature. 1965 Nov 20;208(5012):794–795. doi: 10.1038/208794a0. [DOI] [PubMed] [Google Scholar]
  10. Leguit P., Meinesz A., Huismans L., Eijsvoagel V. P. The use of an antigen-cocktail in the lymphocyte transformation test. Clin Exp Immunol. 1973 May;14(1):149–152. [PMC free article] [PubMed] [Google Scholar]
  11. Mangi R. J., Mardiney M. R., Jr Purification of human lymphocytes by simple centrifugation. Rev Eur Etud Clin Biol. 1970 Oct;15(8):911–912. [PubMed] [Google Scholar]
  12. Mangi R. J., Mardiney M. R., Jr The in vitro transformation of frozen-stored lymphocytes in the mixed lymphocyte reaction and in culture with phytohemagglutinin and specific antigens. J Exp Med. 1970 Sep 1;132(3):401–416. doi: 10.1084/jem.132.3.401. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mangi R. J., Niederman J. C., Kelleher J. E., Jr, Dwyer J. M., Evans A. S., Kantor F. S. Depression of cell-mediated immunity during acute infectious mononucleosis. N Engl J Med. 1974 Nov 28;291(22):1149–1153. doi: 10.1056/NEJM197411282912202. [DOI] [PubMed] [Google Scholar]
  14. McDevitt H. O., Benacerraf B. Genetic control of specific immune responses. Adv Immunol. 1969;11:31–74. doi: 10.1016/s0065-2776(08)60477-0. [DOI] [PubMed] [Google Scholar]
  15. Möller G., Kashiwagi N. Antigen-induced suppression of the in vitro lymphocyte response to different antigens and mitogens. Immunology. 1972 Mar;22(3):441–456. [PMC free article] [PubMed] [Google Scholar]
  16. Osoba D., Falk J. Standard stimulating cells and mixed leukocyte cultures in man. Transplant Proc. 1973 Dec;5(4):1601–1604. [PubMed] [Google Scholar]
  17. Osoba D., Falk J. The mixed-leukocyte reaction in man: effect of pools of stimulating cells selected on the basis of crossreacting HL-A specificities. Cell Immunol. 1974 Jan;10(1):117–135. doi: 10.1016/0008-8749(74)90156-7. [DOI] [PubMed] [Google Scholar]
  18. Segall M., Omodei-Zorini C., Bach F. H., Jorgensen F., Kissmeyer-Nielsen F. HL-A antigens and mixed leukocyte culture reactivity. Transplant Proc. 1973 Mar;5(1):383–389. [PubMed] [Google Scholar]
  19. Wilson D. B., Nowell P. C. Quantitative studies on the mixed lymphocyte interaction in rats. IV. Immunologic potentiality of the responding cells. J Exp Med. 1970 Feb;131(2):391–407. doi: 10.1084/jem.131.2.391. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wilson D. B. Quantitative studies on the mixed lymphocyte interaction in rats. I. Conditions and parameters of response. J Exp Med. 1967 Oct 1;126(4):625–654. doi: 10.1084/jem.126.4.625. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wilson D. B., Silvers W. K., Nowell P. C. Quantitative studies on the mixed lymphocyte interaction in rats. II. Relationship of the proliferative response to the immunologic status of the donors. J Exp Med. 1967 Oct 1;126(4):655–665. doi: 10.1084/jem.126.4.655. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Yale Journal of Biology and Medicine are provided here courtesy of Yale Journal of Biology and Medicine

RESOURCES