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. 1980 Jan-Feb;53(1):41–45.

Recent contributions of molecular biology to the clinical virology of myxoviruses.

E D Kilbourne
PMCID: PMC2595840  PMID: 6990635

Abstract

Recent advances in the clinical virology of influenza are based on non-pragmatically oriented research on the genetics and biochemistry of the influenza virus. Antigenically hybrid recombinant viruses can be tailored to provide monospecific reagents for serological studies. Basic research on viral structure and the mechanism of viral replication has directly influenced the establishment of a cell culture system suitable for the isolation of most influenza viruses. Identification of viral genotype by RNA gel electrophoresis and mapping of oligonucleotides of viral RNA has already facilitated epidemiologic investigations. The clinical virologist of the future must have an understanding of the potential limitations of these techniques for specific strain identification.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Appleyard G., Maber H. B. Plaque formation by influenza viruses in the presence of trypsin. J Gen Virol. 1974 Dec;25(3):351–357. doi: 10.1099/0022-1317-25-3-351. [DOI] [PubMed] [Google Scholar]
  2. Came P. E., Pascale A., Shimonaski G. Effect of pancreatin on plaque formation by influenza viruses. Arch Gesamte Virusforsch. 1968;23(4):346–352. doi: 10.1007/BF01242130. [DOI] [PubMed] [Google Scholar]
  3. Couch R. B., Kasel J. A., Gerin J. L., Schulman J. L., Kilbourne E. D. Induction of partial immunity to influenza by a neuraminidase-specific vaccine. J Infect Dis. 1974 Apr;129(4):411–420. doi: 10.1093/infdis/129.4.411. [DOI] [PubMed] [Google Scholar]
  4. Desselberger U., Nakajima K., Alfino P., Pedersen F. S., Haseltine W. A., Hannoun C., Palese P. Biochemical evidence that "new" influenza virus strains in nature may arise by recombination (reassortment). Proc Natl Acad Sci U S A. 1978 Jul;75(7):3341–3345. doi: 10.1073/pnas.75.7.3341. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gaush C. R., Smith T. F. Replication and plaque assay of influenza virus in an established line of canine kidney cells. Appl Microbiol. 1968 Apr;16(4):588–594. doi: 10.1128/am.16.4.588-594.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ito H., Morimoto Y., Iwase I., Doi Y., Sanpe T. Effect of trypsin on viral susceptibility of Vero cell cultures--cercopithecus kidney line. Jpn J Med Sci Biol. 1970 Aug;23(4):227–235. doi: 10.7883/yoken1952.23.227. [DOI] [PubMed] [Google Scholar]
  7. Kilbourne E. D. Comparative efficacy of neuraminidase-specific and conventional influenza virus vaccines in induction of antibody to neuraminidase in humans. J Infect Dis. 1976 Oct;134(4):384–394. doi: 10.1093/infdis/134.4.384. [DOI] [PubMed] [Google Scholar]
  8. Kilbourne E. D. Genetic dimorphism in influenza viruses: characterization of stably associated hemagglutinin mutants differing in antigenicity and biological properties. Proc Natl Acad Sci U S A. 1978 Dec;75(12):6258–6262. doi: 10.1073/pnas.75.12.6258. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kilbourne E. D. The molecular epidemiology of influenza. J Infect Dis. 1973 Apr;127(4):478–487. doi: 10.1093/infdis/127.4.478. [DOI] [PubMed] [Google Scholar]
  10. Laver W. G., Webster R. G. Studies on the origin of pandemic influenza. II. Peptide maps of the light and heavy polypeptide chains from the hemagglutinin subunits of A 2 influenza viruses isolated before and after the appearance of Hong Kong influenza. Virology. 1972 May;48(2):445–455. doi: 10.1016/0042-6822(72)90055-4. [DOI] [PubMed] [Google Scholar]
  11. Lazarowitz S. G., Choppin P. W. Enhancement of the infectivity of influenza A and B viruses by proteolytic cleavage of the hemagglutinin polypeptide. Virology. 1975 Dec;68(2):440–454. doi: 10.1016/0042-6822(75)90285-8. [DOI] [PubMed] [Google Scholar]
  12. Lazarowitz S. G., Compans R. W., Choppin P. W. Proteolytic cleavage of the hemagglutinin polypeptide of influenza virus. Function of the uncleaved polypeptide HA. Virology. 1973 Mar;52(1):199–212. doi: 10.1016/0042-6822(73)90409-1. [DOI] [PubMed] [Google Scholar]
  13. Linnemann C. C., Jr, Buchman T. G., Light I. J., Ballard J. L. Transmission of herpes-simplex virus type 1 in a nursery for the newborn. Identification of viral isolates by D.N.A. "fingerprinting". Lancet. 1978 May 6;1(8071):964–966. doi: 10.1016/s0140-6736(78)90251-9. [DOI] [PubMed] [Google Scholar]
  14. MADIN S. H., DARBY N. B., Jr Established kidney cell lines of normal adult bovine and ovine origin. Proc Soc Exp Biol Med. 1958 Jul;98(3):574–576. doi: 10.3181/00379727-98-24111. [DOI] [PubMed] [Google Scholar]
  15. Meguro H., Bryant J. D., Torrence A. E., Wright P. F. Canine kidney cell line for isolation of respiratory viruses. J Clin Microbiol. 1979 Feb;9(2):175–179. doi: 10.1128/jcm.9.2.175-179.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Nakajima K., Desselberger U., Palese P. Recent human influenza A (H1N1) viruses are closely related genetically to strains isolated in 1950. Nature. 1978 Jul 27;274(5669):334–339. doi: 10.1038/274334a0. [DOI] [PubMed] [Google Scholar]
  17. Ogra P. L., Chow T., Beutner K. R., Rubi E., Strussenberg J., DeMello S., Rizzone C. Clinical and immunologic evaluation of neuraminidase-specific influenza A virus vaccine in humans. J Infect Dis. 1977 Apr;135(4):499–506. doi: 10.1093/infdis/135.4.499. [DOI] [PubMed] [Google Scholar]
  18. Palese P., Schulman J. L. Mapping of the influenza virus genome: identification of the hemagglutinin and the neuraminidase genes. Proc Natl Acad Sci U S A. 1976 Jun;73(6):2142–2146. doi: 10.1073/pnas.73.6.2142. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Palese P., Schulman J. L. RNA pattern of 'swine' influenza virus isolated from man is similar to those of other swine influenza viruses. Nature. 1976 Oct 7;263(5577):528–530. doi: 10.1038/263528a0. [DOI] [PubMed] [Google Scholar]
  20. Palese P. The genes of influenza virus. Cell. 1977 Jan;10(1):1–10. doi: 10.1016/0092-8674(77)90133-7. [DOI] [PubMed] [Google Scholar]
  21. Roizman B., Buchman T. The molecular epidemiology of herpes simplex viruses. Hosp Pract. 1979 Jan;14(1):95–104. doi: 10.1080/21548331.1979.11707470. [DOI] [PubMed] [Google Scholar]
  22. SUGIURA A., KILBOURNE E. D. GENETIC STUDIES OF INFLUENZA VIRUSES. II. PLAQUE FORMATION BY INFLUENZA VIRUSES IN A CLONE OF A VARIANT HUMAN HETEROPLOID CELL LINE. Virology. 1965 Jul;26:478–488. doi: 10.1016/0042-6822(65)90010-3. [DOI] [PubMed] [Google Scholar]
  23. Schulman J. L., Kilbourne E. D. Independent variation in nature of hemagglutinin and neuraminidase antigens of influenza virus: distinctiveness of hemagglutinin antigen of Hong Kong-68 virus. Proc Natl Acad Sci U S A. 1969 Jun;63(2):326–333. doi: 10.1073/pnas.63.2.326. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Skehel J. J., Waterfield M. D. Studies on the primary structure of the influenza virus hemagglutinin. Proc Natl Acad Sci U S A. 1975 Jan;72(1):93–97. doi: 10.1073/pnas.72.1.93. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Tobita K., Kilbourne E. D. Genetic recombination for antigenic markers of antigenically different strains of influenza B virus. J Virol. 1974 Feb;13(2):347–352. doi: 10.1128/jvi.13.2.347-352.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Tobita K., Sugiura A., Enomote C., Furuyama M. Plaque assay and primary isolation of influenza A viruses in an established line of canine kidney cells (MDCK) in the presence of trypsin. Med Microbiol Immunol. 1975 Dec 30;162(1):9–14. doi: 10.1007/BF02123572. [DOI] [PubMed] [Google Scholar]
  27. WONG S. C., KILBOURNE E. D. Changing viral susceptibility of a human cell line in continuous cultivation. I. Production of infective virus in a variant of the Chang conjunctival cell following infection with swine or N-WS influenza viruses. J Exp Med. 1961 Jan 1;113:95–110. doi: 10.1084/jem.113.1.95. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Webster R. G. Antigenic hybrids of influenza A viruses with surface antigens to order. Virology. 1970 Nov;42(3):633–642. doi: 10.1016/0042-6822(70)90309-0. [DOI] [PubMed] [Google Scholar]
  29. Young J. F., Palese P. Evolution of human influenza A viruses in nature: recombination contributes to genetic variation of H1N1 strains. Proc Natl Acad Sci U S A. 1979 Dec;76(12):6547–6551. doi: 10.1073/pnas.76.12.6547. [DOI] [PMC free article] [PubMed] [Google Scholar]

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