Skip to main content
NCBI home page
The Yale Journal of Biology and Medicine logoLink to The Yale Journal of Biology and Medicine
. 1981 Mar-Apr;54(2):89–94.

The proliferative capacity of pure red cell aplasia bone marrow cells.

L J Katz, R Hoffman, A K Ritchey, N Dainiak
PMCID: PMC2595869  PMID: 7269642

Abstract

Pure red cell aplasia (PRCA) is a heterogeneous disorder. Immunologic abnormalities have recently been uncovered suggesting that both cell-mediated and humoral immune mechanisms may be of etiological importance in PRCA. Utilizing a technique for the cloning of bone marrow erythroid precursors, we determined the in vitro proliferative capacity of erythroid cells obtained from 21 patients with PRCA. Bone marrow cells from one group of patients produced normal or increased numbers of erythroid colonies while the in vitro proliferative capacity of bone marrow cells from a second group was characterized by subnormal erythroid colony formation. Sera obtained from the former group was frequently associated with demonstrable serum inhibitors of erythropoiesis, while PRCA in the latter group was probably the consequence of intrinsic erythroid stem cell defects or pathologic cellular interactions with nonerythroid regulatory cells. This survey of a relatively large population of patients with PRCA provides evidence for the multiple causative mechanisms that can be operative in the production of PRCA.

Full text

PDF
89

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beard M. E., Krantz S. B., Johnson S. A., Bateman C. J., Whitehouse J. M. Pure red cell aplasia. Q J Med. 1978 Jul;47(187):339–348. [PubMed] [Google Scholar]
  2. Browman G. P., Freedman M. H., Blajchman M. A., McBride J. A. A complement independent erythropoietic inhibitor acting on the progenitor cell in refractory anemia. Am J Med. 1976 Oct;61(4):572–578. doi: 10.1016/0002-9343(76)90340-5. [DOI] [PubMed] [Google Scholar]
  3. Böyum A. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest Suppl. 1968;97:77–89. [PubMed] [Google Scholar]
  4. Diamond L. K., Wang W. C., Alter B. P. Congenital hypoplastic anemia. Adv Pediatr. 1976;22:349–378. [PubMed] [Google Scholar]
  5. Hoffman R., Kopel S., Hsu S. D., Dainiak N., Zanjani E. D. T cell chronic lymphocytic leukemia: presence in bone marrow and peripheral blood of cells that suppress erythropoiesis in vitro. Blood. 1978 Jul;52(1):255–260. [PubMed] [Google Scholar]
  6. Hoffman R., Zanjani E. D., Lutton J. D., Zalusky R., Wasserman L. R. Suppression of erythroid-colony formation by lymphocytes from patients with aplastic anemia. N Engl J Med. 1977 Jan 6;296(1):10–13. doi: 10.1056/NEJM197701062960103. [DOI] [PubMed] [Google Scholar]
  7. Koenig H. M., Lightsey A. L., Nelson D. P., Diamond L. K. Immune suppression of erythropoiesis in transient erythroblastopenia of childhood. Blood. 1979 Sep;54(3):742–746. [PubMed] [Google Scholar]
  8. Krantz S. B. Diagnosis and treatment of pure red cell aplasia. Med Clin North Am. 1976 Sep;60(5):945–958. doi: 10.1016/s0025-7125(16)31841-7. [DOI] [PubMed] [Google Scholar]
  9. Krantz S. B., Kao V. Studies on red cell aplasia. I. Demonstration of a plasma inhibitor to heme synthesis and an antibody to erythroblast nuclei. Proc Natl Acad Sci U S A. 1967 Aug;58(2):493–500. doi: 10.1073/pnas.58.2.493. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Krantz S. B., Moore W. H., Zaentz S. D. Studies on red cell aplasia. V. Presence of erythroblast cytotoxicity in G-globulin fraction of plasma. J Clin Invest. 1973 Feb;52(2):324–336. doi: 10.1172/JCI107188. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Stephenson J. R., Axelrad A. A., McLeod D. L., Shreeve M. M. Induction of colonies of hemoglobin-synthesizing cells by erythropoietin in vitro. Proc Natl Acad Sci U S A. 1971 Jul;68(7):1542–1546. doi: 10.1073/pnas.68.7.1542. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Tepperman A. D., Curtis J. E., McCulloch E. A. Erythropietic colonies in cultures of human marrow. Blood. 1974 Nov;44(5):659–669. [PubMed] [Google Scholar]
  13. Wranne L. Transient erythroblastopenia in infancy and childhood. Scand J Haematol. 1970;7(2):76–81. doi: 10.1111/j.1600-0609.1970.tb01872.x. [DOI] [PubMed] [Google Scholar]
  14. Zalusky R., Zanjani E. D., Gidari A. S., Ross J. Site of action of a serum inhibitor of erythropoiesis. J Lab Clin Med. 1973 Jun;81(6):867–875. [PubMed] [Google Scholar]

Articles from The Yale Journal of Biology and Medicine are provided here courtesy of Yale Journal of Biology and Medicine

RESOURCES