Abstract
The phenazine pigments pyocyanin and 1-hydroxyphenazine were resolved by high-pressure liquid chromatography from the sputum sol phase from 9 of 13 patients with cystic fibrosis or bronchiectasis colonized by Pseudomonas aeruginosa. The concentrations measured were each sufficient to inhibit ciliary beating in vitro and contributed a significant proportion of sol phase toxicity for respiratory epithelium.
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- Armstrong A. V., Stewart-Tull D. E., Roberts J. S. Characterisation of the Pseudomonas aeruginosa factor that inhibits mouse-liver mitochondrial respiration. J Med Microbiol. 1971 May;4(2):249–262. doi: 10.1099/00222615-4-2-249. [DOI] [PubMed] [Google Scholar]
- CRUICKSHANK C. N., LOWBURY E. J. The effect of pyocyanin on human skin cells and leucocytes. Br J Exp Pathol. 1953 Dec;34(6):583–587. [PMC free article] [PubMed] [Google Scholar]
- Cox C. D. Role of pyocyanin in the acquisition of iron from transferrin. Infect Immun. 1986 Apr;52(1):263–270. doi: 10.1128/iai.52.1.263-270.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hingley S. T., Hastie A. T., Kueppers F., Higgins M. L., Weinbaum G., Shryock T. Effect of ciliostatic factors from Pseudomonas aeruginosa on rabbit respiratory cilia. Infect Immun. 1986 Jan;51(1):254–262. doi: 10.1128/iai.51.1.254-262.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nutman J., Berger M., Chase P. A., Dearborn D. G., Miller K. M., Waller R. L., Sorensen R. U. Studies on the mechanism of T cell inhibition by the Pseudomonas aeruginosa phenazine pigment pyocyanine. J Immunol. 1987 May 15;138(10):3481–3487. [PubMed] [Google Scholar]
- Rutland J., Cole P. J. Non-invasive sampling of nasal cilia for measurement of beat frequency and study of ultrastructure. Lancet. 1980 Sep 13;2(8194):564–565. doi: 10.1016/s0140-6736(80)91995-9. [DOI] [PubMed] [Google Scholar]
- Smallman L. A., Hill S. L., Stockley R. A. Reduction of ciliary beat frequency in vitro by sputum from patients with bronchiectasis: a serine proteinase effect. Thorax. 1984 Sep;39(9):663–667. doi: 10.1136/thx.39.9.663. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Suter S., Schaad U. B., Roux L., Nydegger U. E., Waldvogel F. A. Granulocyte neutral proteases and Pseudomonas elastase as possible causes of airway damage in patients with cystic fibrosis. J Infect Dis. 1984 Apr;149(4):523–531. doi: 10.1093/infdis/149.4.523. [DOI] [PubMed] [Google Scholar]
- Sykes D. A., Wilson R., Greenstone M., Currie D. C., Steinfort C., Cole P. J. Deleterious effects of purulent sputum sol on human ciliary function in vitro: at least two factors identified. Thorax. 1987 Apr;42(4):256–261. doi: 10.1136/thx.42.4.256. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tegner H., Ohlsson K., Toremalm N. G., von Mecklenburg C. Effect of human leukocyte enzymes on tracheal mucosa and its mucociliary activity. Rhinology. 1979 Sep;17(3):199–206. [PubMed] [Google Scholar]
- Watson D., MacDermot J., Wilson R., Cole P. J., Taylor G. W. Purification and structural analysis of pyocyanin and 1-hydroxyphenazine. Eur J Biochem. 1986 Sep 1;159(2):309–313. doi: 10.1111/j.1432-1033.1986.tb09869.x. [DOI] [PubMed] [Google Scholar]
- Wilson R., Pitt T., Taylor G., Watson D., MacDermot J., Sykes D., Roberts D., Cole P. Pyocyanin and 1-hydroxyphenazine produced by Pseudomonas aeruginosa inhibit the beating of human respiratory cilia in vitro. J Clin Invest. 1987 Jan;79(1):221–229. doi: 10.1172/JCI112787. [DOI] [PMC free article] [PubMed] [Google Scholar]