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. 1988 Dec;56(12):3076–3080. doi: 10.1128/iai.56.12.3076-3080.1988

Serum antibody and ocular responses to murine corneal infection caused by Pseudomonas aeruginosa.

R S Berk 1, I N Montgomery 1, L D Hazlett 1
PMCID: PMC259704  PMID: 3141279

Abstract

The serum antibody response and differential corneal response to primary and secondary infections by Pseudomonas aeruginosa were investigated in DBA/2J (resistant) and C57BL/6J (susceptible) mice, since they respond differently to intracorneal challenge. Using an enzyme-linked immunosorbent assay, we found that naturally resistant DBA/2J mice mounted a significant immunoglobulin M (IgM) and IgG response to P. aeruginosa within 7 days postinfection of one eye; this was subsequently followed by a drop in the IgM response. Of 31 mice, 30 were able to restore corneal clarity within 3 to 4 weeks. However, when C57BL/6J mice were infected intracorneally, their levels of serum antibody developed more slowly than did those of the DBA/2J mice, and they were unable to restore corneal clarity within 8 to 12 weeks. None of the mice from either test strain mounted a detectable serum IgA response to P. aeruginosa over a 90-day holding period. However, infection of the contralateral, normal cornea of mice of both test strains resulted in a heightened IgG response to P. aeruginosa within 30 days after the secondary infection. Many (50%) of the susceptible C57BL/6J mice recovered or exhibited less severe corneal damage within the 30-day holding period. If the C57BL/6J mice were reinfected 60 days after the primary infection instead of after 30 days, most (89%) of the mice had restored corneal clarity within 3 to 6 days. Passive transfer of immune serum from either recovered DBA/2J or C57BL/6J mice to naive C57BL/6J mice resulted in the restoration of corneal clarity in many of the recipients following infection.

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Selected References

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  1. Aizuss D. H., Mondino B. J., Sumner H. L., Dethlefs B. A. The complement system and host defense against Pseudomonas endophthalmitis. Invest Ophthalmol Vis Sci. 1985 Sep;26(9):1262–1266. [PubMed] [Google Scholar]
  2. Allansmith M. R., McClellan B. H. Immunoglobulins in the human cornea. Am J Ophthalmol. 1975 Jul;80(1):123–132. doi: 10.1016/0002-9394(75)90882-x. [DOI] [PubMed] [Google Scholar]
  3. Beisel K. W., Hazlett L. D., Berk R. S. Dominant susceptibility effect on the murine corneal response to Pseudomonas aeruginosa. Proc Soc Exp Biol Med. 1983 Apr;172(4):488–491. doi: 10.3181/00379727-172-41592. [DOI] [PubMed] [Google Scholar]
  4. Berk R. S., Beisel K., Hazlett L. D. Genetic studies of the murine corneal response to Pseudomonas aeruginosa. Infect Immun. 1981 Oct;34(1):1–5. doi: 10.1128/iai.34.1.1-5.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Berk R. S., Hazlett L. D., Beisel K. W. Genetic studies on resistant and susceptibility genes controlling the mouse cornea to infection with Pseudomonas aeruginosa. Antibiot Chemother (1971) 1987;39:83–91. doi: 10.1159/000414336. [DOI] [PubMed] [Google Scholar]
  6. Berk R. S., Iglewski B. H., Hazlett L. D. Age-related susceptibility of mice to ocular challenge with Pseudomonas aeruginosa exotoxin A. Infect Immun. 1981 Jul;33(1):90–94. doi: 10.1128/iai.33.1.90-94.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Berk R. S., Leon M. A., Hazlett L. D. Genetic control of the murine corneal response to Pseudomonas aeruginosa. Infect Immun. 1979 Dec;26(3):1221–1223. doi: 10.1128/iai.26.3.1221-1223.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cleveland R. P., Hazlett L. D., Leon M. A., Berk R. S. Role of complement in murine corneal infection caused by Pseudomonas aeruginosa. Invest Ophthalmol Vis Sci. 1983 Feb;24(2):237–242. [PubMed] [Google Scholar]
  9. Cryz S. J., Jr, Fürer E., Germanier R. Protection against Pseudomonas aeruginosa infection in a murine burn wound sepsis model by passive transfer of antitoxin A, antielastase, and antilipopolysaccharide. Infect Immun. 1983 Mar;39(3):1072–1079. doi: 10.1128/iai.39.3.1072-1079.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Groves M. G., Rosenstreich D. L., Taylor B. A., Osterman J. V. Host defenses in experimental scrub typhus: mapping the gene that controls natural resistance in mice. J Immunol. 1980 Sep;125(3):1395–1399. [PubMed] [Google Scholar]
  11. Hazlett L. D., Rosen D. D., Berk R. S. Age-related susceptibility to Pseudomonas aeruginosa ocular infections in mice. Infect Immun. 1978 Apr;20(1):25–29. doi: 10.1128/iai.20.1.25-29.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hazlett L. D., Rosen D. D., Berk R. S. Pseudomonas eye infections in cyclophosphamide-treated mice. Invest Ophthalmol Vis Sci. 1977 Jul;16(7):649–652. [PubMed] [Google Scholar]
  13. Kreger A. S., Lyerly D. M., Hazlett L. D., Berk R. S. Immunization against experimental Pseudomonas aeruginosa and Serratia marcescens keratitis. Vaccination with lipopolysaccharide endotoxins and proteases. Invest Ophthalmol Vis Sci. 1986 Jun;27(6):932–939. [PubMed] [Google Scholar]
  14. Markham R. B., Powderly W. G. Exposure of mice to live Pseudomonas aeruginosa generates protective cell-mediated immunity in the absence of an antibody response. J Immunol. 1988 Mar 15;140(6):2039–2045. [PubMed] [Google Scholar]
  15. Mondino B. J., Brawman-Mintzer O., Adamu S. A. Corneal antibody levels to ribitol teichoic acid in rabbits immunized with staphylococcal antigens using various routes. Invest Ophthalmol Vis Sci. 1987 Sep;28(9):1553–1558. [PubMed] [Google Scholar]
  16. Mondino B. J., Brown S. I., Rabin B. S. Role of complement in corneal inflammation. Trans Ophthalmol Soc U K. 1978 Sep;98(3):363–366. [PubMed] [Google Scholar]
  17. Mondino B. J., Rao H. Complement levels in normal and inflamed aqueous humor. Invest Ophthalmol Vis Sci. 1983 Mar;24(3):380–384. [PubMed] [Google Scholar]
  18. O'Brien A. D., Scher I., Metcalf E. S. Genetically conferred defect in anti-Salmonella antibody formation renders CBA/N mice innately susceptible to Salmonella typhimurium infection. J Immunol. 1981 Apr;126(4):1368–1372. [PubMed] [Google Scholar]
  19. Pavlovskis O. R., Pollack M., Callahan L. T., 3rd, Iglewski B. H. Passive protection by antitoxin in experimental Pseudomonas aeruginosa burn infections. Infect Immun. 1977 Dec;18(3):596–602. doi: 10.1128/iai.18.3.596-602.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Pennington J. E., Small G. J., Lostrom M. E., Pier G. B. Polyclonal and monoclonal antibody therapy for experimental Pseudomonas aeruginosa pneumonia. Infect Immun. 1986 Oct;54(1):239–244. doi: 10.1128/iai.54.1.239-244.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Powderly W. G., Pier G. B., Markham R. B. In vitro T cell-mediated killing of Pseudomonas aeruginosa. IV. Nonresponsiveness in polysaccharide-immunized BALB/c mice is attributable to vinblastine-sensitive suppressor T cells. J Immunol. 1986 Sep 15;137(6):2025–2030. [PubMed] [Google Scholar]
  22. Smolin G., Hyndiuk R. A. Lymphatic drainage from vascularized rabbit cornea. Am J Ophthalmol. 1971 Jul 30;72(1):147–151. doi: 10.1016/0002-9394(71)91605-9. [DOI] [PubMed] [Google Scholar]
  23. Twining S. S., Lohr K. M., Moulder J. E. The immune system in experimental Pseudomonas keratitis. Model and early effects. Invest Ophthalmol Vis Sci. 1986 Apr;27(4):507–515. [PubMed] [Google Scholar]
  24. Van Horn D. L., Davis S. D., Hyndiuk R. A., Pederson H. J. Experimental Pseudomonas keratitis in the rabbit: bacteriologic, clinical, and microscopic observations. Invest Ophthalmol Vis Sci. 1981 Feb;20(2):213–221. [PubMed] [Google Scholar]
  25. Zweerink H. J., Gammon M. C., Hutchison C. F., Jackson J. J., Pier G. B., Puckett J. M., Sewell T. J., Sigal N. H. X-linked immunodeficient mice as a model for testing the protective efficacy of monoclonal antibodies against Pseudomonas aeruginosa. Infect Immun. 1988 May;56(5):1209–1214. doi: 10.1128/iai.56.5.1209-1214.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]

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