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. 1988 Dec;56(12):3301–3304. doi: 10.1128/iai.56.12.3301-3304.1988

Expression of the histidine-rich protein PfHRP1 by knob-positive Plasmodium falciparum is not sufficient for cytoadherence of infected erythrocytes.

E P Rock 1, A J Saul 1, D W Taylor 1, J H Leech 1, J A Sherwood 1, R J Howard 1
PMCID: PMC259739  PMID: 3053455

Abstract

Six culture-adapted knob-positive Plasmodium falciparum parasites, four of which were nonbinding in an in vitro cytoadherence assay, were tested for the presence of the knob-associated histidine-rich protein PfHRP1. Two knob-positive, strongly cytoadherence-positive P. falciparum strains from Aotus monkeys were also examined. All parasites expressed PfHRP1, suggesting that it plays a structural or functional role related to knobs but is not by itself sufficient for cytoadherence of P. falciparum-infected erythrocytes.

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Selected References

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  1. Aley S. B., Sherwood J. A., Howard R. J. Knob-positive and knob-negative Plasmodium falciparum differ in expression of a strain-specific malarial antigen on the surface of infected erythrocytes. J Exp Med. 1984 Nov 1;160(5):1585–1590. doi: 10.1084/jem.160.5.1585. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ardeshir F., Flint J. E., Matsumoto Y., Aikawa M., Reese R. T., Stanley H. cDNA sequence encoding a Plasmodium falciparum protein associated with knobs and localization of the protein to electron-dense regions in membranes of infected erythrocytes. EMBO J. 1987 May;6(5):1421–1427. doi: 10.1002/j.1460-2075.1987.tb02383.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Culvenor J. G., Langford C. J., Crewther P. E., Saint R. B., Coppel R. L., Kemp D. J., Anders R. F., Brown G. V. Plasmodium falciparum: identification and localization of a knob protein antigen expressed by a cDNA clone. Exp Parasitol. 1987 Feb;63(1):58–67. doi: 10.1016/0014-4894(87)90078-6. [DOI] [PubMed] [Google Scholar]
  4. Fraker P. J., Speck J. C., Jr Protein and cell membrane iodinations with a sparingly soluble chloroamide, 1,3,4,6-tetrachloro-3a,6a-diphrenylglycoluril. Biochem Biophys Res Commun. 1978 Feb 28;80(4):849–857. doi: 10.1016/0006-291x(78)91322-0. [DOI] [PubMed] [Google Scholar]
  5. Howard R. J., Barnwell J. W., Rock E. P., Neequaye J., Ofori-Adjei D., Maloy W. L., Lyon J. A., Saul A. Two approximately 300 kilodalton Plasmodium falciparum proteins at the surface membrane of infected erythrocytes. Mol Biochem Parasitol. 1988 Jan 15;27(2-3):207–223. doi: 10.1016/0166-6851(88)90040-0. [DOI] [PubMed] [Google Scholar]
  6. Howard R. J., Barnwell J. W. Solubilization and immunoprecipitation of 125I-labelled antigens from Plasmodium knowlesi schizont-infected erythrocytes using non-ionic, anionic and zwitterionic detergents. Parasitology. 1984 Feb;88(Pt 1):27–36. doi: 10.1017/s0031182000054317. [DOI] [PubMed] [Google Scholar]
  7. Howard R. J., Uni S., Aikawa M., Aley S. B., Leech J. H., Lew A. M., Wellems T. E., Rener J., Taylor D. W. Secretion of a malarial histidine-rich protein (Pf HRP II) from Plasmodium falciparum-infected erythrocytes. J Cell Biol. 1986 Oct;103(4):1269–1277. doi: 10.1083/jcb.103.4.1269. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kilejian A. Characterization of a protein correlated with the production of knob-like protrusions on membranes of erythrocytes infected with Plasmodium falciparum. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4650–4653. doi: 10.1073/pnas.76.9.4650. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kilejian A. Homology between a histidine-rich protein from Plasmodium lophurae and a protein associated with the knob-like protrusions on membranes of erythrocytes infected with Plasmodium falciparum. J Exp Med. 1980 Jun 1;151(6):1534–1538. doi: 10.1084/jem.151.6.1534. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Langreth S. G., Peterson E. Pathogenicity, stability, and immunogenicity of a knobless clone of Plasmodium falciparum in Colombian owl monkeys. Infect Immun. 1985 Mar;47(3):760–766. doi: 10.1128/iai.47.3.760-766.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lanners H. N., Trager W. Comparative infectivity of knobless and knobby clones of Plasmodium falciparum in splenectomized and intact Aotus trivirgatus monkeys. Z Parasitenkd. 1984;70(6):739–745. doi: 10.1007/BF00927126. [DOI] [PubMed] [Google Scholar]
  12. Leech J. H., Barnwell J. W., Aikawa M., Miller L. H., Howard R. J. Plasmodium falciparum malaria: association of knobs on the surface of infected erythrocytes with a histidine-rich protein and the erythrocyte skeleton. J Cell Biol. 1984 Apr;98(4):1256–1264. doi: 10.1083/jcb.98.4.1256. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Leech J. H., Barnwell J. W., Miller L. H., Howard R. J. Identification of a strain-specific malarial antigen exposed on the surface of Plasmodium falciparum-infected erythrocytes. J Exp Med. 1984 Jun 1;159(6):1567–1575. doi: 10.1084/jem.159.6.1567. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Raventos-Suarez C., Kaul D. K., Macaluso F., Nagel R. L. Membrane knobs are required for the microcirculatory obstruction induced by Plasmodium falciparum-infected erythrocytes. Proc Natl Acad Sci U S A. 1985 Jun;82(11):3829–3833. doi: 10.1073/pnas.82.11.3829. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Schmidt J. A., Udeinya I. J., Leech J. H., Hay R. J., Aikawa M., Barnwell J., Green I., Miller L. H. Plasmodium falciparum malaria. An amelanotic melanoma cell line bears receptors for the knob ligand on infected erythrocytes. J Clin Invest. 1982 Aug;70(2):379–386. doi: 10.1172/JCI110627. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Taylor D. W., Parra M., Chapman G. B., Stearns M. E., Rener J., Aikawa M., Uni S., Aley S. B., Panton L. J., Howard R. J. Localization of Plasmodium falciparum histidine-rich protein 1 in the erythrocyte skeleton under knobs. Mol Biochem Parasitol. 1987 Sep;25(2):165–174. doi: 10.1016/0166-6851(87)90005-3. [DOI] [PubMed] [Google Scholar]
  17. Trager W., Jensen J. B. Human malaria parasites in continuous culture. Science. 1976 Aug 20;193(4254):673–675. doi: 10.1126/science.781840. [DOI] [PubMed] [Google Scholar]
  18. Udeinya I. J., Graves P. M., Carter R., Aikawa M., Miller L. H. Plasmodium falciparum: effect of time in continuous culture on binding to human endothelial cells and amelanotic melanoma cells. Exp Parasitol. 1983 Oct;56(2):207–214. doi: 10.1016/0014-4894(83)90064-4. [DOI] [PubMed] [Google Scholar]
  19. Udeinya I. J., Schmidt J. A., Aikawa M., Miller L. H., Green I. Falciparum malaria-infected erythrocytes specifically bind to cultured human endothelial cells. Science. 1981 Jul 31;213(4507):555–557. doi: 10.1126/science.7017935. [DOI] [PubMed] [Google Scholar]
  20. Vernot-Hernandez J. P., Heidrich H. G. The relationship to knobs of the 92,000 D protein specific for knobby strains of Plasmodium falciparum. Z Parasitenkd. 1985;71(1):41–51. doi: 10.1007/BF00932917. [DOI] [PubMed] [Google Scholar]

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