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Infection and Immunity logoLink to Infection and Immunity
. 1988 Dec;56(12):3329–3333. doi: 10.1128/iai.56.12.3329-3333.1988

In vitro model for Campylobacter pylori adherence properties.

V Neman-Simha 1, F Mégraud 1
PMCID: PMC259747  PMID: 3182085

Abstract

The adherence of 12 strains of Campylobacter pylori was studied on four cell lines. Immunofluorescence and scanning and transmission electron microscopy were used to visualize the bacteria. A heavy adherence to the epithelial cell line HEp-2 and to the intestinal cell line Int-407 was noted. By transmission electron microscopy, a close association between bacteria and cells in the form of cup-like structures was observed, but pedestals were not present.

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Selected References

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  1. Chen X. G., Correa P., Offerhaus J., Rodriguez E., Janney F., Hoffmann E., Fox J., Hunter F., Diavolitsis S. Ultrastructure of the gastric mucosa harboring Campylobacter-like organisms. Am J Clin Pathol. 1986 Nov;86(5):575–582. doi: 10.1093/ajcp/86.5.575. [DOI] [PubMed] [Google Scholar]
  2. Duvert M., Mazat J. P., Barets A. L. Intermitochondrial junctions in the heart of the frog, Rana esculenta. A thin-section and freeze-fracture study. Cell Tissue Res. 1985;241(1):129–137. doi: 10.1007/BF00214634. [DOI] [PubMed] [Google Scholar]
  3. Goodwin C. S., Armstrong J. A., Marshall B. J. Campylobacter pyloridis, gastritis, and peptic ulceration. J Clin Pathol. 1986 Apr;39(4):353–365. doi: 10.1136/jcp.39.4.353. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Johnson W. M., Lior H. Cytotoxic and cytotonic factors produced by Campylobacter jejuni, Campylobacter coli, and Campylobacter laridis. J Clin Microbiol. 1986 Aug;24(2):275–281. doi: 10.1128/jcm.24.2.275-281.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kasper G., Dickgiesser N. Isolation from gastric epithelium of Campylobacter-like bacteria that are distinct from "Campylobacter pyloridis". Lancet. 1985 Jan 12;1(8420):111–112. doi: 10.1016/s0140-6736(85)92005-7. [DOI] [PubMed] [Google Scholar]
  6. Knutton S., Baldini M. M., Kaper J. B., McNeish A. S. Role of plasmid-encoded adherence factors in adhesion of enteropathogenic Escherichia coli to HEp-2 cells. Infect Immun. 1987 Jan;55(1):78–85. doi: 10.1128/iai.55.1.78-85.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Laboisse C. L., Augeron C., Couturier-Turpin M. H., Gespach C., Cheret A. M., Potet F. Characterization of a newly established human gastric cancer cell line HGT-1 bearing histamine H2-receptors. Cancer Res. 1982 Apr;42(4):1541–1548. [PubMed] [Google Scholar]
  8. Marshall B. J., Warren J. R. Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration. Lancet. 1984 Jun 16;1(8390):1311–1315. doi: 10.1016/s0140-6736(84)91816-6. [DOI] [PubMed] [Google Scholar]
  9. Megraud F., Bonnet F., Garnier M., Lamouliatte H. Characterization of "Campylobacter pyloridis" by culture, enzymatic profile, and protein content. J Clin Microbiol. 1985 Dec;22(6):1007–1010. doi: 10.1128/jcm.22.6.1007-1010.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Rosen E. M., Mueller S. N., Noveral J. P., Levine E. M. Proliferative characteristics of clonal endothelial cell strains. J Cell Physiol. 1981 Apr;107(1):123–137. doi: 10.1002/jcp.1041070114. [DOI] [PubMed] [Google Scholar]
  11. Scaletsky I. C., Silva M. L., Trabulsi L. R. Distinctive patterns of adherence of enteropathogenic Escherichia coli to HeLa cells. Infect Immun. 1984 Aug;45(2):534–536. doi: 10.1128/iai.45.2.534-536.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Steele T. W., Sangster N., Lanser J. A. DNA relatedness and biochemical features of Campylobacter spp. isolated in central and South Australia. J Clin Microbiol. 1985 Jul;22(1):71–74. doi: 10.1128/jcm.22.1.71-74.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Tricottet V., Bruneval P., Vire O., Camilleri J. P., Bloch F., Bonte N., Roge J. Campylobacter-like organisms and surface epithelium abnormalities in active, chronic gastritis in humans: an ultrastructural study. Ultrastruct Pathol. 1986;10(2):113–122. doi: 10.3109/01913128609014587. [DOI] [PubMed] [Google Scholar]

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