TABLE 2.
Results of global fitting analysis of stopped-flow fluorescence data to Scheme 1
| Parameter | fitted rate |
|---|---|
| k1 (M−1s−1) | 1.0 × 108 ± 1.0 × 10−3 |
| k2 (s−1) | 1.0 × 104 ± 1.0 × 10−3 |
| k3 (s−1) | 990 ± 1 |
| k4 (s−1) | 2.4 × 10−4 ± 8.6 × 10−7 |
| k5 (s−1) | 163 ± 1 |
| k6 (s−1) | 4.0 × 10−3 ± 1.7 × 10−6 |
| k7 (s−1) | 98 ± 1 |
Substrate used in the stopped-flow studies was imipenem. Data were fitted with Applied Photophysics PC ProK global fitting software using the mechanism in Scheme 1. The reported uncertainties represent standard errors as determined by the ProK software (non-linear Marquardt-Levenberg algorithm).