Table 2.
Characteristics of the Antibodies Used in the Study
| Antibody to | Clone | Source | Dilution | Retrieval | Detection |
|---|---|---|---|---|---|
| Ki-67 | MIB-1 | Dakoa | 1:150 | Steamer/EDTA buffer | Envisiona |
| Caspase-3 (cleaved, ASP-175 rabbit antibody) | Cell Signalingb | 1:250 | Microwave/citrate buffer | ABCc | |
| VEGF-A (A20) (rabbit antibody) | Santa Cruzd | 1:150 (overnight incubation) | Pressure cooker/citrate buffer | Envision+ | |
| VEGF-C (N-19) (goat antibody) | Santa Cruz | 1:250 (overnight incubation) | Steamer/EDTA buffer | LSAB+a | |
| E-Cadherin | 36B5 | Novocastrae | 1:50 | Pressure cooker/citrate buffer | Envision+ |
| EGFR | EGFR.25 | Novocastra | 1:50 | Pressure cooker/citrate buffer | Envision+ |
a
Dako Corp., Carpinteria, CA
b
Cell Signaling Technologies, Inc., Beverly, MA
c
Vector Laboratories, Burlingame, CA
d
Santa Cruz Biotechnology, Inc., Santa Cruz, CA
e
Vision-Biosystems, Norwell, MA