Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1988 May;56(5):1288–1294. doi: 10.1128/iai.56.5.1288-1294.1988

Characterization of P fimbriae on O1, O7, O75, rough, and nontypable strains of Escherichia coli.

A Pere 1, R K Selander 1, T K Korhonen 1
PMCID: PMC259810  PMID: 2895742

Abstract

P fimbriae of 37 uropathogenic Escherichia coli O1:K1, O7:K1, O22, O75, rough:K1, and nontypable strains were characterized by immunoprecipitation with 14 fimbria-specific rabbit antisera. The fimbrial composition of these strains, as reflected by the apparent molecular weights of the fimbrial peptides, was correlated with the O serogroup of the strains, but serological cross-reactivity of P fimbriae of different E. coli serogroups was frequently observed. The genetic clonal relationships of the strains were analyzed by determining the electrophoretic types, based on 18 chromosomally encoded enzymes. Among the O1:K1 strains, the same P-fimbrial variants occurred on strains that were either closely related or very distinct in their electrophoretic types, indicating that the P fimbriae have evolved in association with the O and K antigens. In contrast, certain O7:K1 and R:K1 strains as well as some O22 and O75 strains were genotypically identical and shared similar P-fimbrial variants, which differed serologically from those of other E. coli serogroups. Our results show that, despite the structural variability seen in electrophoretic analysis of P fimbriae of different serogroups, many P-fimbrial variants share common antigenic determinants that are recognized by rabbit antisera. Based on immunoprecipitation analyses, three anti-P-fimbria sera have now been identified that react with P fimbriae of 82 of 84 uropathogenic E. coli strains characterized in Finland.

Full text

PDF
1288

Images in this article

1292Image
on p.1292

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abe C., Schmitz S., Moser I., Boulnois G., High N. J., Orskov I., Orskov F., Jann B., Jann K. Monoclonal antibodies with fimbrial F1C, F12, F13, and F14 specificities obtained with fimbriae from E. coli 04:K12:H-. Microb Pathog. 1987 Jan;2(1):71–77. doi: 10.1016/0882-4010(87)90116-1. [DOI] [PubMed] [Google Scholar]
  2. Achtman M., Heuzenroeder M., Kusecek B., Ochman H., Caugant D., Selander R. K., Väisanen-Rhen V., Korhonen T. K., Stuart S., Orskov F. Clonal analysis of Escherichia coli O2:K1 isolated from diseased humans and animals. Infect Immun. 1986 Jan;51(1):268–276. doi: 10.1128/iai.51.1.268-276.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Achtman M., Mercer A., Kusecek B., Pohl A., Heuzenroeder M., Aaronson W., Sutton A., Silver R. P. Six widespread bacterial clones among Escherichia coli K1 isolates. Infect Immun. 1983 Jan;39(1):315–335. doi: 10.1128/iai.39.1.315-335.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Carbonetti N. H., Boonchai S., Parry S. H., Väisänen-Rhen V., Korhonen T. K., Williams P. H. Aerobactin-mediated iron uptake by Escherichia coli isolates from human extraintestinal infections. Infect Immun. 1986 Mar;51(3):966–968. doi: 10.1128/iai.51.3.966-968.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Caugant D. A., Levin B. R., Orskov I., Orskov F., Svanborg Eden C., Selander R. K. Genetic diversity in relation to serotype in Escherichia coli. Infect Immun. 1985 Aug;49(2):407–413. doi: 10.1128/iai.49.2.407-413.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hull S., Clegg S., Sanborg Eden C., Hull R. Multiple forms of genes in pyelonephritogenic Escherichia coli encoding adhesins binding globoseries glycolipid receptors. Infect Immun. 1985 Jan;47(1):80–83. doi: 10.1128/iai.47.1.80-83.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Korhonen T. K., Virkola R., Holthöfer H. Localization of binding sites for purified Escherichia coli P fimbriae in the human kidney. Infect Immun. 1986 Nov;54(2):328–332. doi: 10.1128/iai.54.2.328-332.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Korhonen T. K., Väisänen-Rhen V., Rhen M., Pere A., Parkkinen J., Finne J. Escherichia coli fimbriae recognizing sialyl galactosides. J Bacteriol. 1984 Aug;159(2):762–766. doi: 10.1128/jb.159.2.762-766.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Korhonen T. K., Väisänen V., Saxén H., Hultberg H., Svenson S. B. P-antigen-recognizing fimbriae from human uropathogenic Escherichia coli strains. Infect Immun. 1982 Jul;37(1):286–291. doi: 10.1128/iai.37.1.286-291.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Källenius G., Möllby R., Svenson S. B., Helin I., Hultberg H., Cedergren B., Winberg J. Occurrence of P-fimbriated Escherichia coli in urinary tract infections. Lancet. 1981 Dec 19;2(8260-61):1369–1372. doi: 10.1016/s0140-6736(81)92797-5. [DOI] [PubMed] [Google Scholar]
  11. Källenius G., Möllby R., Svenson S. B., Winberg J., Hultberg H. Identification of a carbohydrate receptor recognized by uropathogenic Escherichia coli. Infection. 1980;8 (Suppl 3):288–293. doi: 10.1007/BF01639597. [DOI] [PubMed] [Google Scholar]
  12. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  13. O'Hanley P., Lark D., Falkow S., Schoolnik G. Molecular basis of Escherichia coli colonization of the upper urinary tract in BALB/c mice. Gal-Gal pili immunization prevents Escherichia coli pyelonephritis in the BALB/c mouse model of human pyelonephritis. J Clin Invest. 1985 Feb;75(2):347–360. doi: 10.1172/JCI111707. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ochman H., Selander R. K. Evidence for clonal population structure in Escherichia coli. Proc Natl Acad Sci U S A. 1984 Jan;81(1):198–201. doi: 10.1073/pnas.81.1.198. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Orskov I., Orskov F. Serology of Escherichia coli fimbriae. Prog Allergy. 1983;33:80–105. [PubMed] [Google Scholar]
  16. Pere A., Leinonen M., Väisänen-Rhen V., Rhen M., Korhonen T. K. Occurrence of type-1C fimbriae on Escherichia coli strains isolated from human extraintestinal infections. J Gen Microbiol. 1985 Jul;131(7):1705–1711. doi: 10.1099/00221287-131-7-1705. [DOI] [PubMed] [Google Scholar]
  17. Pere A., Nowicki B., Saxén H., Siitonen A., Korhonen T. K. Expression of P, type-1, and type-1C fimbriae of Escherichia coli in the urine of patients with acute urinary tract infection. J Infect Dis. 1987 Oct;156(4):567–574. doi: 10.1093/infdis/156.4.567. [DOI] [PubMed] [Google Scholar]
  18. Pere A., Väisänen-Rhen V., Rhen M., Tenhunen J., Korhonen T. K. Analysis of P fimbriae on Escherichia coli O2, O4, and O6 strains by immunoprecipitation. Infect Immun. 1986 Feb;51(2):618–625. doi: 10.1128/iai.51.2.618-625.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Rhen M., van Die I., Rhen V., Bergmans H. Comparison of the nucleotide sequences of the genes encoding the KS71A and F7(1) fimbrial antigens of uropathogenic Escherichia coli. Eur J Biochem. 1985 Sep 16;151(3):573–577. doi: 10.1111/j.1432-1033.1985.tb09142.x. [DOI] [PubMed] [Google Scholar]
  20. Roberts J. A., Hardaway K., Kaack B., Fussell E. N., Baskin G. Prevention of pyelonephritis by immunization with P-fimbriae. J Urol. 1984 Mar;131(3):602–607. doi: 10.1016/s0022-5347(17)50513-3. [DOI] [PubMed] [Google Scholar]
  21. Selander R. K., Korhonen T. K., Väisänen-Rhen V., Williams P. H., Pattison P. E., Caugant D. A. Genetic relationships and clonal structure of strains of Escherichia coli causing neonatal septicemia and meningitis. Infect Immun. 1986 Apr;52(1):213–222. doi: 10.1128/iai.52.1.213-222.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Väisänen-Rhen V., Elo J., Väisänen E., Siitonen A., Orskov I., Orskov F., Svenson S. B., Mäkelä P. H., Korhonen T. K. P-fimbriated clones among uropathogenic Escherichia coli strains. Infect Immun. 1984 Jan;43(1):149–155. doi: 10.1128/iai.43.1.149-155.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. de Ree J. M., Schwillens P., van den Bosch J. F. Monoclonal antibodies for serotyping the P fimbriae of uropathogenic Escherichia coli. J Clin Microbiol. 1986 Jul;24(1):121–125. doi: 10.1128/jcm.24.1.121-125.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. de Ree J. M., Schwillens P., van den Bosch J. F. Monoclonal antibodies that recognize the P fimbriae F71, F72, F9, and F11 from uropathogenic Escherichia coli. Infect Immun. 1985 Dec;50(3):900–904. doi: 10.1128/iai.50.3.900-904.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. de Ree J. M., van den Bosch J. F. Serological response to the P fimbriae of uropathogenic Escherichia coli in pyelonephritis. Infect Immun. 1987 Sep;55(9):2204–2207. doi: 10.1128/iai.55.9.2204-2207.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. van Die I., Bergmans H. Nucleotide sequence of the gene encoding the F72 fimbrial subunit of a uropathogenic Escherichia coli strain. Gene. 1984 Dec;32(1-2):83–90. doi: 10.1016/0378-1119(84)90035-0. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES