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. 1988 May;56(5):1371–1375. doi: 10.1128/iai.56.5.1371-1375.1988

Mouse macrophages stimulated by recombinant gamma interferon to kill tumor cells are not bactericidal for the facultative intracellular bacterium Listeria monocytogenes.

P A Campbell 1, B P Canono 1, J L Cook 1
PMCID: PMC259835  PMID: 3128484

Abstract

Data presented here demonstrate that recombinant gamma interferon (rIFN-gamma) activated a single population of 10% fetal calf serum-elicited mouse peritoneal exudate cells to express tumoricidal activity but not bactericidal activity for the facultative intracellular bacterium Listeria monocytogenes. Fetal calf serum-elicited cells incubated with rIFN-gamma phagocytosed listeriae normally, suggesting that their inability to kill this bacterium is not because they cannot phagocytose it. Data also show that proteose peptone-elicited peritoneal exudate cells, which are bactericidal but not tumoricidal, acquired tumoricidal activity but lost bactericidal activity following incubation overnight with rIFN-gamma. These experiments show that under conditions sufficient for rIFN-gamma to induce macrophages to express tumoricidal activity, the same cell population does not express bactericidal activity for the facultative intracellular bacterium L. monocytogenes. This suggests that mechanisms responsible for these two biological activities may be different.

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Selected References

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  1. Adams D. O., Hamilton T. A. The cell biology of macrophage activation. Annu Rev Immunol. 1984;2:283–318. doi: 10.1146/annurev.iy.02.040184.001435. [DOI] [PubMed] [Google Scholar]
  2. Bancroft G. J., Schreiber R. D., Bosma G. C., Bosma M. J., Unanue E. R. A T cell-independent mechanism of macrophage activation by interferon-gamma. J Immunol. 1987 Aug 15;139(4):1104–1107. [PubMed] [Google Scholar]
  3. Buchmeier N. A., Schreiber R. D. Requirement of endogenous interferon-gamma production for resolution of Listeria monocytogenes infection. Proc Natl Acad Sci U S A. 1985 Nov;82(21):7404–7408. doi: 10.1073/pnas.82.21.7404. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Campbell P. A., Czuprynski C. J., Cook J. L. Differential expression of macrophage effector functions: bactericidal versus tumoricidal activities. J Leukoc Biol. 1984 Sep;36(3):293–306. doi: 10.1002/jlb.36.3.293. [DOI] [PubMed] [Google Scholar]
  5. Czuprynski C. J., Brown J. F. Purified human and recombinant murine interleukin-1 alpha induced accumulation of inflammatory peritoneal neutrophils and mononuclear phagocytes: possible contributions to antibacterial resistance. Microb Pathog. 1987 Nov;3(5):377–386. doi: 10.1016/0882-4010(87)90007-6. [DOI] [PubMed] [Google Scholar]
  6. Czuprynski C. J., Henson P. M., Campbell P. A. Enhanced accumulation of inflammatory neutrophils and macrophages mediated by transfer of T cells from mice immunized with Listeria monocytogenes. J Immunol. 1985 May;134(5):3449–3454. [PubMed] [Google Scholar]
  7. Douvas G. S., Looker D. L., Vatter A. E., Crowle A. J. Gamma interferon activates human macrophages to become tumoricidal and leishmanicidal but enhances replication of macrophage-associated mycobacteria. Infect Immun. 1985 Oct;50(1):1–8. doi: 10.1128/iai.50.1.1-8.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Flesch I., Kaufmann S. H. Mycobacterial growth inhibition by interferon-gamma-activated bone marrow macrophages and differential susceptibility among strains of Mycobacterium tuberculosis. J Immunol. 1987 Jun 15;138(12):4408–4413. [PubMed] [Google Scholar]
  9. Hopper K. E., Cahill J. M. Immunoregulation by macrophages II. Separation of mouse peritoneal macrophages having tumoricidal and bactericidal activities and those secreting PGE and interleukin I. J Reticuloendothel Soc. 1983 Jun;33(6):443–456. [PubMed] [Google Scholar]
  10. Johnson W. J., Marino P. A., Schreiber R. D., Adams D. O. Sequential activation of murine mononuclear phagocytes for tumor cytolysis: differential expression of markers by macrophages in the several stages of development. J Immunol. 1983 Aug;131(2):1038–1043. [PubMed] [Google Scholar]
  11. Kiderlen A. F., Kaufmann S. H., Lohmann-Matthes M. L. Protection of mice against the intracellular bacterium Listeria monocytogenes by recombinant immune interferon. Eur J Immunol. 1984 Oct;14(10):964–967. doi: 10.1002/eji.1830141019. [DOI] [PubMed] [Google Scholar]
  12. Langford M. P., Stanton G. J., Johnson H. M. Biological effects of staphylococcal enterotoxin A on human peripheral lymphocytes. Infect Immun. 1978 Oct;22(1):62–68. doi: 10.1128/iai.22.1.62-68.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ming W. J., Bersani L., Mantovani A. Tumor necrosis factor is chemotactic for monocytes and polymorphonuclear leukocytes. J Immunol. 1987 Mar 1;138(5):1469–1474. [PubMed] [Google Scholar]
  14. Mishell R. I., Dutton R. W. Immunization of dissociated spleen cell cultures from normal mice. J Exp Med. 1967 Sep 1;126(3):423–442. doi: 10.1084/jem.126.3.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Murray H. W., Spitalny G. L., Nathan C. F. Activation of mouse peritoneal macrophages in vitro and in vivo by interferon-gamma. J Immunol. 1985 Mar;134(3):1619–1622. [PubMed] [Google Scholar]
  16. Nacy C. A., Leonard E. J., Meltzer M. S. Macrophages in resistance to rickettsial infections: characterization of lymphokines that induce rickettsiacidal activity in macrophages. J Immunol. 1981 Jan;126(1):204–207. [PubMed] [Google Scholar]
  17. Nathan C. F., Murray H. W., Wiebe M. E., Rubin B. Y. Identification of interferon-gamma as the lymphokine that activates human macrophage oxidative metabolism and antimicrobial activity. J Exp Med. 1983 Sep 1;158(3):670–689. doi: 10.1084/jem.158.3.670. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Pace J. L., Russell S. W., Torres B. A., Johnson H. M., Gray P. W. Recombinant mouse gamma interferon induces the priming step in macrophage activation for tumor cell killing. J Immunol. 1983 May;130(5):2011–2013. [PubMed] [Google Scholar]
  19. Rook G. A., Steele J., Ainsworth M., Champion B. R. Activation of macrophages to inhibit proliferation of Mycobacterium tuberculosis: comparison of the effects of recombinant gamma-interferon on human monocytes and murine peritoneal macrophages. Immunology. 1986 Nov;59(3):333–338. [PMC free article] [PubMed] [Google Scholar]
  20. Russell S. W., Pace J. L., Varesio L., Akporiaye E., Blasi E., Celado A., Schreiber R. D., Schultz R. M., Stevenson A. P., Stewart C. C. Comparison of five short-term assays that measure nonspecific cytotoxicity mediated to tumor cells by activated macrophages. J Leukoc Biol. 1986 Dec;40(6):801–813. doi: 10.1002/jlb.40.6.801. [DOI] [PubMed] [Google Scholar]
  21. Schreiber R. D., Hicks L. J., Celada A., Buchmeier N. A., Gray P. W. Monoclonal antibodies to murine gamma-interferon which differentially modulate macrophage activation and antiviral activity. J Immunol. 1985 Mar;134(3):1609–1618. [PubMed] [Google Scholar]
  22. Schreiber R. D. Identification of gamma-interferon as a murine macrophage-activating factor for tumor cytotoxicity. Contemp Top Immunobiol. 1984;13:171–198. doi: 10.1007/978-1-4757-1445-6_9. [DOI] [PubMed] [Google Scholar]
  23. Schultz R. M., Kleinschmidt W. J. Functional identity between murine gamma interferon and macrophage activating factor. Nature. 1983 Sep 15;305(5931):239–240. doi: 10.1038/305239a0. [DOI] [PubMed] [Google Scholar]
  24. Turco J., Winkler H. H. Effect of mouse lymphokines and cloned mouse interferon-gamma on the interaction of Rickettsia prowazekii with mouse macrophage-like RAW264.7 cells. Infect Immun. 1984 Aug;45(2):303–308. doi: 10.1128/iai.45.2.303-308.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Unanue E. R. Antigen-presenting function of the macrophage. Annu Rev Immunol. 1984;2:395–428. doi: 10.1146/annurev.iy.02.040184.002143. [DOI] [PubMed] [Google Scholar]
  26. Wing E. J., Gardner I. D., Ryning F. W., Remington J. S. Dissociation of effector functions in populations of activated macrophages. Nature. 1977 Aug 18;268(5621):642–644. doi: 10.1038/268642a0. [DOI] [PubMed] [Google Scholar]
  27. Wing E. J., Krahenbuhl J. L., Remington J. S. Studies of macrophage function during Trichinella spiralis infection in mice. Immunology. 1979 Mar;36(3):479–485. [PMC free article] [PubMed] [Google Scholar]
  28. van Dissel J. T., Stikkelbroeck J. J., Michel B. C., van den Barselaar M. T., Leijh P. C., van Furth R. Inability of recombinant interferon-gamma to activate the antibacterial activity of mouse peritoneal macrophages against Listeria monocytogenes and Salmonella typhimurium. J Immunol. 1987 Sep 1;139(5):1673–1678. [PubMed] [Google Scholar]

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