Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1989 Dec;57(12):3940–3944. doi: 10.1128/iai.57.12.3940-3944.1989

Bacteroides loeschei PK1295 cells express two distinct adhesins simultaneously.

J London 1, A R Hand 1, E I Weiss 1, J Allen 1
PMCID: PMC259930  PMID: 2572563

Abstract

Bacteroides loeschei synthesizes two distinct adhesins that mediate its coaggregation with Streptococcus sanguis 34 and Actinomyces israeli PK14. Streptococcal adhesin-specific and actinomyces adhesin-specific monoclonal antibodies were used to prepare antibody-coated 5- or 10-nm gold particles. These were used in immunoelectron microscopic studies to establish that essentially all bacteroides cells in a population express both adhesins. In general, the two sizes of gold particles representing each type of adhesin appeared to be spatially separated on neighboring fimbriae of B. loeschei. Deposition of antibody-coated gold particles, representing both types of adhesin, at or near the same fimbria was observed less frequently.

Full text

PDF
3940

Images in this article

3941Image
on p.3941
3942Image
on p.3942
3943Image
on p.3943

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abraham S. N., Goguen J. D., Sun D., Klemm P., Beachey E. H. Identification of two ancillary subunits of Escherichia coli type 1 fimbriae by using antibodies against synthetic oligopeptides of fim gene products. J Bacteriol. 1987 Dec;169(12):5530–5536. doi: 10.1128/jb.169.12.5530-5536.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Abraham S. N., Sun D., Dale J. B., Beachey E. H. Conservation of the D-mannose-adhesion protein among type 1 fimbriated members of the family Enterobacteriaceae. Nature. 1988 Dec 15;336(6200):682–684. doi: 10.1038/336682a0. [DOI] [PubMed] [Google Scholar]
  3. BRINTON C. C., Jr Non-flagellar appendages of bacteria. Nature. 1959 Mar 21;183(4664):782–786. doi: 10.1038/183782a0. [DOI] [PubMed] [Google Scholar]
  4. DUGUID J. P., SMITH I. W., DEMPSTER G., EDMUNDS P. N. Non-flagellar filamentous appendages (fimbriae) and haemagglutinating activity in Bacterium coli. J Pathol Bacteriol. 1955 Oct;70(2):335–348. doi: 10.1002/path.1700700210. [DOI] [PubMed] [Google Scholar]
  5. Geoghegan W. D., Ackerman G. A. Adsorption of horseradish peroxidase, ovomucoid and anti-immunoglobulin to colloidal gold for the indirect detection of concanavalin A, wheat germ agglutinin and goat anti-human immunoglobulin G on cell surfaces at the electron microscopic level: a new method, theory and application. J Histochem Cytochem. 1977 Nov;25(11):1187–1200. doi: 10.1177/25.11.21217. [DOI] [PubMed] [Google Scholar]
  6. Geoghegan W. D., Ambegaonkar S., Calvanico N. J. Passive gold agglutination. An alternative to passive hemagglutination. J Immunol Methods. 1980;34(1):11–21. doi: 10.1016/0022-1759(80)90219-7. [DOI] [PubMed] [Google Scholar]
  7. Lund B., Marklund B. I., Strömberg N., Lindberg F., Karlsson K. A., Normark S. Uropathogenic Escherichia coli can express serologically identical pili of different receptor binding specificities. Mol Microbiol. 1988 Mar;2(2):255–263. doi: 10.1111/j.1365-2958.1988.tb00027.x. [DOI] [PubMed] [Google Scholar]
  8. Mooi F. R., van Buuren M., Koopman G., Roosendaal B., de Graaf F. K. K88ab gene of Escherichia coli encodes a fimbria-like protein distinct from the K88ab fimbrial adhesin. J Bacteriol. 1984 Aug;159(2):482–487. doi: 10.1128/jb.159.2.482-487.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Nowicki B., Rhen M., Väisänen-Rhen V., Pere A., Korhonen T. K. Immunofluorescence study of fimbrial phase variation in Escherichia coli KS71. J Bacteriol. 1984 Nov;160(2):691–695. doi: 10.1128/jb.160.2.691-695.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Orskov I., Orskov F., Birch-Andersen A. Comparison of Escherichia coli fimbrial antigen F7 with type 1 fimbriae. Infect Immun. 1980 Feb;27(2):657–666. doi: 10.1128/iai.27.2.657-666.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Uhlin B. E., Norgren M., Båga M., Normark S. Adhesion to human cells by Escherichia coli lacking the major subunit of a digalactoside-specific pilus-adhesin. Proc Natl Acad Sci U S A. 1985 Mar;82(6):1800–1804. doi: 10.1073/pnas.82.6.1800. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Weiss E. I., Kolenbrander P. E., London J., Hand A. R., Andersen R. N. Fimbria-associated proteins of Bacteroides loescheii PK1295 mediate intergeneric coaggregations. J Bacteriol. 1987 Sep;169(9):4215–4222. doi: 10.1128/jb.169.9.4215-4222.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Weiss E. I., London J., Kolenbrander P. E., Andersen R. N. Fimbria-associated adhesin of Bacteroides loeschei that recognizes receptors on procaryotic and eucaryotic cells. Infect Immun. 1989 Sep;57(9):2912–2913. doi: 10.1128/iai.57.9.2912-2913.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Weiss E. I., London J., Kolenbrander P. E., Andersen R. N., Fischler C., Siraganian R. P. Characterization of monoclonal antibodies to fimbria-associated adhesins of Bacteroides loescheii PK1295. Infect Immun. 1988 Jan;56(1):219–224. doi: 10.1128/iai.56.1.219-224.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Weiss E. I., London J., Kolenbrander P. E., Hand A. R., Siraganian R. Localization and enumeration of fimbria-associated adhesins of Bacteroides loescheii. J Bacteriol. 1988 Mar;170(3):1123–1128. doi: 10.1128/jb.170.3.1123-1128.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES