Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1987 Nov;55(11):2590–2596. doi: 10.1128/iai.55.11.2590-2596.1987

Antigenic identity of biologically active antigens in coccidioidin and spherulin.

R A Cox 1, L A Britt 1
PMCID: PMC259947  PMID: 3666954

Abstract

We recently reported the isolation of three clinically relevant antigens from coccidioidin; viz., the antigen that is reactive in the immunodiffusion (ID) assay for detecting tube precipitin (TP) antibody (designated IDTP); the antigen that is reactive in the ID assay for detecting complement-fixing antibody (designated IDCF); and the heat-stable (HS) antigen which, when demonstrated in soluble extracts of fungal cultures by using the IDHS assay, establishes the mycologic identification of Coccidioides immitis. This investigation was undertaken to determine whether the IDTP, IDCF, and IDHS antigens isolated from coccidioidin are of antigenic identity with components in spherulin. By employing the purified coccidioidin antigens in line-immunoelectrophoresis with spherulin and by assaying coccidioidin and spherulin by tandem two-dimensional immunoelectrophoresis against antisera produced to the purified antigens, we report that these three coccidioidin-derived antigens are antigenically identical to precipitinogens in spherulin.

Full text

PDF
2590

Images in this article

2591Image
on p.2591
2592Image
on p.2592
2593Image
on p.2593
2593Image
on p.2593
2594Image
on p.2594
2594Image
on p.2594
2595Image
on p.2595

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Axelsen N. H. Intermediate gel in crossed and in fused rocket immunoelectrophoresis. Scand J Immunol Suppl. 1973;1:71–77. doi: 10.1111/j.1365-3083.1973.tb03782.x. [DOI] [PubMed] [Google Scholar]
  2. Calhoun D. L., Osir E. O., Dugger K. O., Galgiani J. N., Law J. H. Humoral antibody responses to specific antigens of Coccidioides immitis. J Infect Dis. 1986 Aug;154(2):265–272. doi: 10.1093/infdis/154.2.265. [DOI] [PubMed] [Google Scholar]
  3. Cox R. A., Britt L. A. Antigenic heterogeneity of an alkali-soluble, water-soluble cell wall extract of Coccidioides immitis. Infect Immun. 1985 Nov;50(2):365–369. doi: 10.1128/iai.50.2.365-369.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cox R. A., Britt L. A. Isolation and identification of an exoantigen specific for Coccidioides immitis. Infect Immun. 1986 Apr;52(1):138–143. doi: 10.1128/iai.52.1.138-143.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cox R. A., Britt L. A. Isolation of a coccidioidin component that reacts with immunoglobulin M precipitin antibody. Infect Immun. 1986 Sep;53(3):449–453. doi: 10.1128/iai.53.3.449-453.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cox R. A., Britt L. A., Michael R. A. Isolation of Coccidioides immitis F antigen by immunoaffinity chromatography with monospecific antiserum. Infect Immun. 1987 Jan;55(1):227–232. doi: 10.1128/iai.55.1.227-232.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. DiSalvo A. F., Terreni A. A., Wooten A. K. Use of the exoantigen test to identify blastomyces dermatitidis, coccidioides immitis, and Histoplasma capsulatum in mixed cultures. Am J Clin Pathol. 1981 Jun;75(6):825–826. doi: 10.1093/ajcp/75.6.825. [DOI] [PubMed] [Google Scholar]
  8. Drutz D. J., Catanzaro A. Coccidioidomycosis. Part I. Am Rev Respir Dis. 1978 Mar;117(3):559–585. doi: 10.1164/arrd.1978.117.3.559. [DOI] [PubMed] [Google Scholar]
  9. Emmett M., Crowle A. J. Crossed immunoelectrophoresis: qualitative and quantitative considerations. J Immunol Methods. 1982;50(2):R65–R83. doi: 10.1016/0022-1759(82)90218-6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Guinet R., Gabriel S. Antigen overlay technique for line immunoelectrophoresis and crossed-line immunoelectrophoresis of thermolabile antigens. J Immunol Methods. 1982;51(1):29–33. doi: 10.1016/0022-1759(82)90378-7. [DOI] [PubMed] [Google Scholar]
  11. HEINER D. C. Diagnosis of histoplasmosis using precipitin reactions in agargel. Pediatrics. 1958 Oct;22(4 Pt 1):616–627. [PubMed] [Google Scholar]
  12. Huppert M., Spratt N. S., Vukovich K. R., Sun S. H., Rice E. H. Antigenic analysis of coccidioidin and spherulin determined by two-dimensional immunoelectrophoresis. Infect Immun. 1978 May;20(2):541–551. doi: 10.1128/iai.20.2.541-551.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Huppert M., Sun S. H., Harrison J. L. Morphogenesis throughout saprobic and parasitic cycles of Coccidioides immitis. Mycopathologia. 1982 May 22;78(2):107–122. doi: 10.1007/BF00442634. [DOI] [PubMed] [Google Scholar]
  14. Huppert M., Sun S. H., Rice E. H. Specificity of exoantigens for identifying cultures of Coccidioides immitis. J Clin Microbiol. 1978 Sep;8(3):346–348. doi: 10.1128/jcm.8.3.346-348.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kaufman L., Clark M. J. Value of the concomitant use of complement fixation and immunodiffusion tests in the diagnosis of coccidioidomycosis. Appl Microbiol. 1974 Oct;28(4):641–643. doi: 10.1128/am.28.4.641-643.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kaufman L., Standard P. G., Huppert M., Pappagianis D. Comparison and diagnostic value of the coccidioidin heat-stable (HS and tube precipitin) antigens in immunodiffusion. J Clin Microbiol. 1985 Oct;22(4):515–518. doi: 10.1128/jcm.22.4.515-518.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Levine H. B., Cobb J. M., Scalarone G. M. Spherule coccidioidin in delayed dermal sensitivity reactions of experimental animals. Sabouraudia. 1969 Feb;7(1):20–32. doi: 10.1080/00362177085190051. [DOI] [PubMed] [Google Scholar]
  18. Levine H. B., Gonzalez-Ochoa A., Ten Eyck D. R. Dermal sensitivity to Coccidioides immitis. A comparison of responses elicited in man by spherulin and coccidioidin. Am Rev Respir Dis. 1973 Mar;107(3):379–386. doi: 10.1164/arrd.1973.107.3.379. [DOI] [PubMed] [Google Scholar]
  19. PAPPAGIANIS D., SMITH C. E., KOBAYASHI G. S., SAITO M. T. Studies of antigens from young mycelia of Coccidioides immitis. J Infect Dis. 1961 Jan-Feb;108:35–44. doi: 10.1093/infdis/108.1.35. [DOI] [PubMed] [Google Scholar]
  20. SMITH C. E., SAITO M. T., BEARD R. R., KEPP R. M., CLARK R. W., EDDIE B. U. Serological tests in the diagnosis and prognosis of coccidioidomycosis. Am J Hyg. 1950 Jul;52(1):1–21. doi: 10.1093/oxfordjournals.aje.a119404. [DOI] [PubMed] [Google Scholar]
  21. SMITH C. E., SAITO M. T., SIMONS S. A. Pattern of 39,500 serologic tests in coccidioidomycosis. J Am Med Assoc. 1956 Feb 18;160(7):546–552. doi: 10.1001/jama.1956.02960420026008. [DOI] [PubMed] [Google Scholar]
  22. Scalarone G. M., Levine H. B., Pappagianis D., Chaparas S. D. Spherulin as a complement-fixing antigen in human coccidioidomycosis. Am Rev Respir Dis. 1974 Sep;110(3):324–328. doi: 10.1164/arrd.1974.110.3.324. [DOI] [PubMed] [Google Scholar]
  23. Sun S. H., Huppert M., Vukovich K. R. Rapid in vitro conversion and identification of Coccidioides immitis. J Clin Microbiol. 1976 Feb;3(2):186–190. doi: 10.1128/jcm.3.2.186-190.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Zimmer B. L., Pappagianis D. Comparison of immunoblot analyses of spherule-endospore-phase extracellular protein and mycelial-phase antigen of Coccidioides immitis. Infect Immun. 1986 Jul;53(1):64–70. doi: 10.1128/iai.53.1.64-70.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES