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. 1987 Nov;55(11):2669–2674. doi: 10.1128/iai.55.11.2669-2674.1987

Characterization of cyclophosphamide-rat model of cryptosporidiosis.

J E Rehg 1, M L Hancock 1, D B Woodmansee 1
PMCID: PMC259959  PMID: 3666958

Abstract

A cyclophosphamide-treated-rat model for cryptosporidiosis is described. The optimal conditions for inducing a cryptosporidial infection in rats were a 50 mg/kg dose of cyclophosphamide per day in the drinking water for a minimum of 14 days before inoculation with 10(4) or more infectious oocysts. Eighteen days after inoculation, 80% or more of the animals were infected. Cryptosporidia were attached to the terminal 24 cm of the small intestine, and the immunosuppressed animals remained infected as long as they received cyclophosphamide. However, the intestinal infection cleared within 7 to 10 days after cyclophosphamide was withdrawn. Oocysts continued to be detected in the feces for an additional 5 to 7 days after cryptosporidia were no longer visibly attached to the ideal enterocytes. This study characterized an animal model that can be used to investigate the pathogenesis of and evaluate therapeutic regimens for cryptosporidiosis in the immunocompromised host.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Current W. L., Reese N. C., Ernst J. V., Bailey W. S., Heyman M. B., Weinstein W. M. Human cryptosporidiosis in immunocompetent and immunodeficient persons. Studies of an outbreak and experimental transmission. N Engl J Med. 1983 May 26;308(21):1252–1257. doi: 10.1056/NEJM198305263082102. [DOI] [PubMed] [Google Scholar]
  2. Heine J., Moon H. W., Woodmansee D. B. Persistent Cryptosporidium infection in congenitally athymic (nude) mice. Infect Immun. 1984 Mar;43(3):856–859. doi: 10.1128/iai.43.3.856-859.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hughes W. T., Smith B. L., Jacobus D. P. Successful treatment and prevention of murine Pneumocystis carinii pneumonitis with 4,4'-sulfonylbisformanilide. Antimicrob Agents Chemother. 1986 Mar;29(3):509–510. doi: 10.1128/aac.29.3.509. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hurme M., Bång B. E., Sihvola M. Genetic differences in the cyclophosphamide-induced immune suppression: weaker suppression of T-cell cytotoxicity by cyclophosphamide activated by CBA mice. Clin Immunol Immunopathol. 1980 Sep;17(1):38–42. doi: 10.1016/0090-1229(80)90071-9. [DOI] [PubMed] [Google Scholar]
  5. Long P. L., Rose M. E. Extended schizogony of Eimeria mivati in betamethasone-treated chickens. Parasitology. 1970 Feb;60(1):147–155. doi: 10.1017/s0031182000077313. [DOI] [PubMed] [Google Scholar]
  6. Moon H. W., Woode G. N., Ahrens F. A. Attempted chemoprophylaxis of cryptosporidiosis in calves. Vet Rec. 1982 Feb 20;110(8):181–181. doi: 10.1136/vr.110.8.181-a. [DOI] [PubMed] [Google Scholar]
  7. Niilo L. The effect of dexamethasone on bovine coccidiosis. Can J Comp Med. 1970 Oct;34(4):325–328. [PMC free article] [PubMed] [Google Scholar]
  8. Reese N. C., Current W. L., Ernst J. V., Bailey W. S. Cryptosporidiosis of man and calf: a case report and results of experimental infections in mice and rats. Am J Trop Med Hyg. 1982 Mar;31(2):226–229. doi: 10.4269/ajtmh.1982.31.226. [DOI] [PubMed] [Google Scholar]
  9. Sherwood D., Angus K. W., Snodgrass D. R., Tzipori S. Experimental cryptosporidiosis in laboratory mice. Infect Immun. 1982 Nov;38(2):471–475. doi: 10.1128/iai.38.2.471-475.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Sloper K. S., Dourmashkin R. R., Bird R. B., Slavin G., Webster A. D. Chronic malabsorption due to cryptosporidiosis in a child with immunoglobulin deficiency. Gut. 1982 Jan;23(1):80–82. doi: 10.1136/gut.23.1.80. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Soave R., Danner R. L., Honig C. L., Ma P., Hart C. C., Nash T., Roberts R. B. Cryptosporidiosis in homosexual men. Ann Intern Med. 1984 Apr;100(4):504–511. doi: 10.7326/0003-4819-100-4-504. [DOI] [PubMed] [Google Scholar]
  12. Stockman G. D., Heim L. R., South M. A., Trentin J. J. Differential effects of cyclophosphamide on the B and T cell compartments of adult mice. J Immunol. 1973 Jan;110(1):277–282. [PubMed] [Google Scholar]
  13. Tzipori S. R., Campbell I., Angus K. W. The therapeutic effect of 16 antimicrobial agents on Cryptosporidium infection in mice. Aust J Exp Biol Med Sci. 1982 Apr;60(Pt 2):187–190. doi: 10.1038/icb.1982.20. [DOI] [PubMed] [Google Scholar]
  14. Tzipori S., Angus K. W., Campbell I., Gray E. W. Cryptosporidium: evidence for a single-species genus. Infect Immun. 1980 Dec;30(3):884–886. doi: 10.1128/iai.30.3.884-886.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Tzipori S. Cryptosporidiosis in animals and humans. Microbiol Rev. 1983 Mar;47(1):84–96. doi: 10.1128/mr.47.1.84-96.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Tzipori S., Smith M., Makin T., Halpin C. Enterocolitis in piglets caused by Cryptosporidium sp. purified from calf faeces. Vet Parasitol. 1982 Nov;11(2-3):121–126. doi: 10.1016/0304-4017(82)90033-4. [DOI] [PubMed] [Google Scholar]

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