Skip to main content
Infection and Immunity logoLink to Infection and Immunity
. 1987 Nov;55(11):2754–2758. doi: 10.1128/iai.55.11.2754-2758.1987

Cytoadherence of Plasmodium falciparum-infected erythrocytes to human melanoma cell lines correlates with surface OKM5 antigen.

L J Panton 1, J H Leech 1, L H Miller 1, R J Howard 1
PMCID: PMC259972  PMID: 3312010

Abstract

OKM5 antigen and thrombospondin are currently under investigation as potential receptors on the surface of human monocytes, endothelial cells, and melanomas responsible for the cytoadherence of Plasmodium falciparum-infected erythrocytes. We have studies the binding capacity of six human melanoma cell lines and related this property to the cytoplasmic and surface expression of the OKM5 antigen and thrombospondin by using indirect immunofluorescence assays on methanol-fixed and nonfixed melanomas. The presence of OKM5 antigen was detectable only in the melanoma lines which bound P. falciparum-infected erythrocytes. Thrombospondin was present in the cytoplasm of all the melanoma lines but was not detectable on the surface of any cells. Our work demonstrates a direct correlation between surface OKM5 antigen and cytoadherence in vitro. While our results do not exclude thrombospondin as a mediator of cytoadherence to endothelial cells in vivo, they showed no correlation between the presence of thrombospondin and the ability of melanoma cell lines to cytoadhere in vitro.

Full text

PDF
2754

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barnwell J. W., Howard R. J., Coon H. G., Miller L. H. Splenic requirement for antigenic variation and expression of the variant antigen on the erythrocyte membrane in cloned Plasmodium knowlesi malaria. Infect Immun. 1983 Jun;40(3):985–994. doi: 10.1128/iai.40.3.985-994.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barnwell J. W., Howard R. J., Miller L. H. Influence of the spleen on the expression of surface antigens on parasitized erythrocytes. Ciba Found Symp. 1983;94:117–136. doi: 10.1002/9780470715444.ch8. [DOI] [PubMed] [Google Scholar]
  3. Barnwell J. W., Ockenhouse C. F., Knowles D. M., 2nd Monoclonal antibody OKM5 inhibits the in vitro binding of Plasmodium falciparum-infected erythrocytes to monocytes, endothelial, and C32 melanoma cells. J Immunol. 1985 Nov;135(5):3494–3497. [PubMed] [Google Scholar]
  4. Dixit V. M., Galvin N. J., O'Rourke K. M., Frazier W. A. Monoclonal antibodies that recognize calcium-dependent structures of human thrombospondin. Characterization and mapping of their epitopes. J Biol Chem. 1986 Feb 5;261(4):1962–1968. [PubMed] [Google Scholar]
  5. Geiman Q. M., Meagher M. J. Susceptibility of a New World monkey to Plasmodium falciparum from man. Nature. 1967 Jul 22;215(5099):437–439. doi: 10.1038/215437a0. [DOI] [PubMed] [Google Scholar]
  6. Jaffe E. A., Ruggiero J. T., Leung L. K., Doyle M. J., McKeown-Longo P. J., Mosher D. F. Cultured human fibroblasts synthesize and secrete thrombospondin and incorporate it into extracellular matrix. Proc Natl Acad Sci U S A. 1983 Feb;80(4):998–1002. doi: 10.1073/pnas.80.4.998. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jensen J. B., Trager W. Plasmodium falciparum in culture: establishment of additional strains. Am J Trop Med Hyg. 1978 Jul;27(4):743–746. doi: 10.4269/ajtmh.1978.27.743. [DOI] [PubMed] [Google Scholar]
  8. Johnson G. D., Nogueira Araujo G. M. A simple method of reducing the fading of immunofluorescence during microscopy. J Immunol Methods. 1981;43(3):349–350. doi: 10.1016/0022-1759(81)90183-6. [DOI] [PubMed] [Google Scholar]
  9. Knowles D. M., 2nd, Tolidjian B., Marboe C., D'Agati V., Grimes M., Chess L. Monoclonal anti-human monocyte antibodies OKM1 and OKM5 possess distinctive tissue distributions including differential reactivity with vascular endothelium. J Immunol. 1984 May;132(5):2170–2173. [PubMed] [Google Scholar]
  10. Lawler J. The structural and functional properties of thrombospondin. Blood. 1986 May;67(5):1197–1209. [PubMed] [Google Scholar]
  11. Ockenhouse C. F., Schulman S., Shear H. L. Induction of crisis forms in the human malaria parasite Plasmodium falciparum by gamma-interferon-activated, monocyte-derived macrophages. J Immunol. 1984 Sep;133(3):1601–1608. [PubMed] [Google Scholar]
  12. Roberts D. D., Sherwood J. A., Spitalnik S. L., Panton L. J., Howard R. J., Dixit V. M., Frazier W. A., Miller L. H., Ginsburg V. Thrombospondin binds falciparum malaria parasitized erythrocytes and may mediate cytoadherence. Nature. 1985 Nov 7;318(6041):64–66. doi: 10.1038/318064a0. [DOI] [PubMed] [Google Scholar]
  13. Sherwood J. A., Roberts D. D., Marsh K., Harvey E. B., Spitalnik S. L., Miller L. H., Howard R. J. Thrombospondin binding by parasitized erythrocyte isolates in falciparum malaria. Am J Trop Med Hyg. 1987 Mar;36(2):228–233. doi: 10.4269/ajtmh.1987.36.228. [DOI] [PubMed] [Google Scholar]
  14. Silverstein R. L., Leung L. L., Nachman R. L. Thrombospondin: a versatile multifunctional glycoprotein. Arteriosclerosis. 1986 May-Jun;6(3):245–253. doi: 10.1161/01.atv.6.3.245. [DOI] [PubMed] [Google Scholar]
  15. Talle M. A., Rao P. E., Westberg E., Allegar N., Makowski M., Mittler R. S., Goldstein G. Patterns of antigenic expression on human monocytes as defined by monoclonal antibodies. Cell Immunol. 1983 May;78(1):83–99. doi: 10.1016/0008-8749(83)90262-9. [DOI] [PubMed] [Google Scholar]
  16. Trager W., Jensen J. B. Human malaria parasites in continuous culture. Science. 1976 Aug 20;193(4254):673–675. doi: 10.1126/science.781840. [DOI] [PubMed] [Google Scholar]
  17. Udeinya I. J., Leech J., Aikawa M., Miller L. H. An in vitro assay for sequestration: binding of Plasmodium falciparum-infected erythrocytes to formalin-fixed endothelial cells and amelanotic melanoma cells. J Protozool. 1985 Feb;32(1):88–90. doi: 10.1111/j.1550-7408.1985.tb03019.x. [DOI] [PubMed] [Google Scholar]
  18. Udeinya I. J., Schmidt J. A., Aikawa M., Miller L. H., Green I. Falciparum malaria-infected erythrocytes specifically bind to cultured human endothelial cells. Science. 1981 Jul 31;213(4507):555–557. doi: 10.1126/science.7017935. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES