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. 1987 Nov;55(11):2774–2776. doi: 10.1128/iai.55.11.2774-2776.1987

Transposon Tn5 mutagenesis of Brucella abortus.

L D Smith 1, F Heffron 1
PMCID: PMC259975  PMID: 2822580

Abstract

We demonstrate that transposon-mediated mutagenesis can be used to construct mutations in the pathogen Brucella abortus. We have used both a plasmid and a bacteriophage to introduce either Tn5 or Tn5 lac into the Brucella chromosome. B. abortus is naturally sensitive to kanamycin. We have selected 22 independent kanamycin-resistant colonies in strain US-19 and 19 colonies in strain 2308. We have demonstrated by Southern hybridization that Tn5 was inserted into the chromosome.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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