Abstract
Antibody to the capsular polysaccharide of Haemophilus influenzae type b (Hib) was not protective in infant rats depleted of complement by cobra venom factor (CoVF) even when serum antibody levels were many times the minimum protective level. Partial protection from Hib infection was achieved in CoVF-treated rats only if they were passively hyperimmunized with large doses of immunoglobulin G Hib capsular polysaccharide antibody. In addition, nonimmune CoVF-treated rats had higher mortality and blood bacterial density than nonimmune rats with intact complement systems.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Anderson P. Intrinsic tritium labeling of the capsular polysaccharide antigen of Haemophilus influenzae type B. J Immunol. 1978 Mar;120(3):866–870. [PubMed] [Google Scholar]
- Anderson P., Johnston R. B., Jr, Smith D. H. Human serum activities against Hemophilus influenzae, type b. J Clin Invest. 1972 Jan;51(1):31–38. doi: 10.1172/JCI106793. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Axén R., Porath J., Ernback S. Chemical coupling of peptides and proteins to polysaccharides by means of cyanogen halides. Nature. 1967 Jun 24;214(5095):1302–1304. doi: 10.1038/2141302a0. [DOI] [PubMed] [Google Scholar]
- Cates K. L., Marsh K. H., Granoff D. M. Serum opsonic activity after immunization of adults with Haemophilus influenzae type b-diphtheria toxoid conjugate vaccine. Infect Immun. 1985 Apr;48(1):183–189. doi: 10.1128/iai.48.1.183-189.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Crosson F. J., Jr, Winkelstein J. A., Moxon E. R. Participation of complement in the nonimmune host defense against experimental Haemophilus influenzae type b septicemia and meningitis. Infect Immun. 1976 Oct;14(4):882–887. doi: 10.1128/iai.14.4.882-887.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cuatrecasas P., Wilchek M., Anfinsen C. B. Selective enzyme purification by affinity chromatography. Proc Natl Acad Sci U S A. 1968 Oct;61(2):636–643. doi: 10.1073/pnas.61.2.636. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ehlenberger A. G., Nussenzweig V. The role of membrane receptors for C3b and C3d in phagocytosis. J Exp Med. 1977 Feb 1;145(2):357–371. doi: 10.1084/jem.145.2.357. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hosea S. W., Brown E. J., Frank M. M. The critical role of complement in experimental pneumococcal sepsis. J Infect Dis. 1980 Dec;142(6):903–909. doi: 10.1093/infdis/142.6.903. [DOI] [PubMed] [Google Scholar]
- Johnston R. B., Jr, Anderson P., Rosen F. S., Smith D. H. Characterization of human antibody to polyribophosphate, the capsular antigen of Hemophilus influenzae, type B. Clin Immunol Immunopathol. 1973 Jan;1(2):234–240. doi: 10.1016/0090-1229(73)90024-x. [DOI] [PubMed] [Google Scholar]
- Levy N. J., Kasper D. L. Antibody-independent and -dependent opsonization of group B Streptococcus requires the first component of complement C1. Infect Immun. 1985 Jul;49(1):19–24. doi: 10.1128/iai.49.1.19-24.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- March S. C., Parikh I., Cuatrecasas P. A simplified method for cyanogen bromide activation of agarose for affinity chromatography. Anal Biochem. 1974 Jul;60(1):149–152. doi: 10.1016/0003-2697(74)90139-0. [DOI] [PubMed] [Google Scholar]
- Newman S. L., Waldo B., Johnston R. B., Jr Separation of serum bactericidal and opsonizing activities for Haemophilus influenzae type b. Infect Immun. 1973 Sep;8(3):488–490. doi: 10.1128/iai.8.3.488-490.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Robbins J. B., Parke J. C., Jr, Schneerson R., Whisnant J. K. Quantitative measurement of "natural" and immunization-induced Haemophilus influenzae type b capsular polysaccharide antibodies. Pediatr Res. 1973 Mar;7(3):103–110. doi: 10.1203/00006450-197303000-00001. [DOI] [PubMed] [Google Scholar]
- Schneerson R., Rodrigues L. P., Parke J. C., Jr, Robbins J. B. Immunity to disease caused by Hemophilus influenzae type b. II. Specificity and some biologic characteristics of "natural," infection-acquired, and immunization-induced antibodies to the capsular polysaccharide of Hemophilus influenzae type b. J Immunol. 1971 Oct;107(4):1081–1089. [PubMed] [Google Scholar]
- Schreiber J. R., Barrus V. A., Siber G. R. Decreased protective efficacy of reduced and alkylated human immune serum globulin in experimental infection with Haemophilus influenzae type b. Infect Immun. 1985 Jan;47(1):142–148. doi: 10.1128/iai.47.1.142-148.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schreiber J. R., Barrus V., Cates K. L., Siber G. R. Functional characterization of human IgG, IgM, and IgA antibody directed to the capsule of Haemophilus influenzae type b. J Infect Dis. 1986 Jan;153(1):8–16. doi: 10.1093/infdis/153.1.8. [DOI] [PubMed] [Google Scholar]
- Siber G. R., Ambrosino D. M., McIver J., Ervin T. J., Schiffman G., Sallan S., Grady G. F. Preparation of human hyperimmune globulin to Haemophilus influenzae b, Streptococcus pneumoniae, and Neisseria meningitidis. Infect Immun. 1984 Jul;45(1):248–254. doi: 10.1128/iai.45.1.248-254.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Steele N. P., Munson R. S., Jr, Granoff D. M., Cummins J. E., Levine R. P. Antibody-dependent alternative pathway killing of Haemophilus influenzae type b. Infect Immun. 1984 May;44(2):452–458. doi: 10.1128/iai.44.2.452-458.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sutton A., Schneerson R., Kendall-Morris S., Robbins J. B. Differential complement resistance mediates virulence of Haemophilus influenzae type b. Infect Immun. 1982 Jan;35(1):95–104. doi: 10.1128/iai.35.1.95-104.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tarr P. I., Hosea S. W., Brown E. J., Schneerson R., Sutton A., Frank M. M. The requirement of specific anticapsular IgG for killing of Haemophilus influenzae by the alternative pathway of complement activation. J Immunol. 1982 Apr;128(4):1772–1775. [PubMed] [Google Scholar]
- Weller P. F., Smith A. L., Smith D. H., Anderson P. Role of immunity in the clearance of bacteremia due to Haemophilus influenzae. J Infect Dis. 1978 Oct;138(4):427–436. doi: 10.1093/infdis/138.4.427. [DOI] [PubMed] [Google Scholar]