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. 1987 Dec;55(12):2967–2976. doi: 10.1128/iai.55.12.2967-2976.1987

A potassium thiocyanate extract vaccine prepared from Pasteurella multocida 3:A protects rabbits against homologous challenge.

Y S Lu 1, S P Pakes 1, L Massey 1, C Stefanu 1
PMCID: PMC260015  PMID: 3679540

Abstract

Potassium thiocyanate extracts of a virulent Pasteurella multocida 3:A rabbit isolate were prepared and used as a vaccine in rabbits. The extract contained protein, carbohydrate, hyaluronic acid, lipopolysaccharide, DNA, and RNA. The protein and lipopolysaccharide profiles of the extract were similar to those of the P. multocida cell membrane. Rabbits were vaccinated intranasally (i.n.) or intramuscularly (i.m.) four times at 1- or 3-week intervals and challenged i.n. with the homologous P. multocida 2 weeks after the last vaccination. Rabbits vaccinated with the extract by the i.n. route developed persisting serum immunoglobulin G (IgG) and nasal IgA antibodies, whereas rabbits immunized by the i.m. route produced persisting serum IgG and transient nasal IgA antibodies. The extract prevents the death of rabbits which were vaccinated by either route and challenged. Vaccination by the i.n. route in rabbits reduced the numbers of virulent P. multocida in nasal cavities and lungs and the prevalence and severity of rhinitis and pneumonia. These i.n.-vaccinated rabbits were also resistant to virulent P. multocida colonization in liver, spleen, uterus, and tympanic bullae. Similarly, i.m. vaccination in rabbits resulted in a reduction in the severity of rhinitis; the numbers of virulent P. multocida in lungs; and the prevalence of colonization in liver, spleen, uterus, and tympanic bullae. Vaccination by the i.n. route was superior to that by the i.m. route in that there was a significant reduction in the severity of pneumonia and numbers of virulent P. multocida in nasal cavities and lungs. Rabbits vaccinated with the extract without challenge showed no lesions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALEXANDER M. M., SAWIN P. B., ROEHM D. A. Respiratory infection in the rabbit: an enzootic caused by Pasteurella lepiseptica and attempts to control it by vaccination. J Infect Dis. 1952 Jan-Feb;90(1):30–33. doi: 10.1093/infdis/90.1.30. [DOI] [PubMed] [Google Scholar]
  2. Ames G. F. Resolution of bacterial proteins by polyacrylamide gel electrophoresis on slabs. Membrane, soluble, and periplasmic fractions. J Biol Chem. 1974 Jan 25;249(2):634–644. [PubMed] [Google Scholar]
  3. BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  5. Brogden K. A. Physiological and serological characteristics of 48 Pasteurella multocida cultures from rabbits. J Clin Microbiol. 1980 Jun;11(6):646–649. doi: 10.1128/jcm.11.6.646-649.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chengappa M. M., Myers R. C., Carter G. R. A streptomycin dependent live Pasteurella multocida vaccine for the prevention of rabbit pasteurellosis. Lab Anim Sci. 1980 Jun;30(3):515–518. [PubMed] [Google Scholar]
  7. Chengappa M. M., Myers R. C., Carter G. R. Capsular and somatic types of Pasteurella multocida from rabbits. Can J Comp Med. 1982 Oct;46(4):437–439. [PMC free article] [PubMed] [Google Scholar]
  8. Gaunt G., Moffat R., Mukkur T. K. Fowl cholera: immunization of chickens with potassium thiocyanate (KSCN) extract of Pasteurella multocida serotype 3. Avian Dis. 1977 Oct-Dec;21(4):543–548. [PubMed] [Google Scholar]
  9. Greenberg H., Helms C. M., Grizzard M. B., James W. D., Horswood R. L., Chanock R. M. Immunoprophylaxis of experimental Mycoplasma pneumoniae disease: effect of route of administration on the immunogenicity and protective effect of inactivated M. pneumoniae vaccine. Infect Immun. 1977 Apr;16(1):88–92. doi: 10.1128/iai.16.1.88-92.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gulig P. A., McCracken G. H., Jr, Frisch C. F., Johnston K. H., Hansen E. J. Antibody response of infants to cell surface-exposed outer membrane proteins of Haemophilus influenzae type b after systemic Haemophilus disease. Infect Immun. 1982 Jul;37(1):82–88. doi: 10.1128/iai.37.1.82-88.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jaslow B. W., Ringler D. H., Rush H. G., Glorioso J. C. Pasteurella associated rhinitis of rabbits: efficacy of penicillin therapy. Lab Anim Sci. 1981 Aug;31(4):382–385. [PubMed] [Google Scholar]
  12. Karkhanis Y. D., Zeltner J. Y., Jackson J. J., Carlo D. J. A new and improved microassay to determine 2-keto-3-deoxyoctonate in lipopolysaccharide of Gram-negative bacteria. Anal Biochem. 1978 Apr;85(2):595–601. doi: 10.1016/0003-2697(78)90260-9. [DOI] [PubMed] [Google Scholar]
  13. Lu Y. S., Pakes S. P. Protection of rabbits against experimental pasteurellosis by a streptomycin-dependent Pasteurella multocida serotype 3:A live mutant vaccine. Infect Immun. 1981 Dec;34(3):1018–1024. doi: 10.1128/iai.34.3.1018-1024.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lu Y. S., Pakes S. P., Stefanu C. Capsular and somatic serotypes of Pasteurella multocida isolates recovered from healthy and diseased rabbits in Texas. J Clin Microbiol. 1983 Aug;18(2):292–295. doi: 10.1128/jcm.18.2.292-295.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Manning P. J., Naasz M. A., DeLong D., Leary S. L. Pasteurellosis in laboratory rabbits: characterization of lipopolysaccharides of Pasteurella multocida by polyacrylamide gel electrophoresis, immunoblot techniques, and enzyme-linked immunosorbent assay. Infect Immun. 1986 Sep;53(3):460–463. doi: 10.1128/iai.53.3.460-463.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Manning P. J. Naturally occurring pasteurellosis in laboratory rabbits: chemical and serological studies of whole cells and lipopolysaccharides of Pasteurella multocida. Infect Immun. 1984 May;44(2):502–507. doi: 10.1128/iai.44.2.502-507.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. McKinney K. L., Rebers P. A. Purification of Pasteurella multocida antigens by ultracentrifugation and isoelectrofocusing. Can J Microbiol. 1982 May;28(5):511–521. doi: 10.1139/m82-077. [DOI] [PubMed] [Google Scholar]
  18. McKinney K. L., Rebers P. A., Rimler R. B. Immunogenicity of potassium thiocyanate extracted and electrofocused Pasteurella multocida X-73I antigens in chickens and mice. Can J Microbiol. 1982 Nov;28(11):1219–1225. doi: 10.1139/m82-182. [DOI] [PubMed] [Google Scholar]
  19. Mukkur T. K. Immunogenicity of a chaotropically extracted protective antigen(s) of Pasteurella multocida type A (bovine origin) against experimental pasteurellosis in mice. J Gen Microbiol. 1979 Jul;113(1):37–43. doi: 10.1099/00221287-113-1-37. [DOI] [PubMed] [Google Scholar]
  20. Mukkur T. K. Immunologic and physiologic responses of calves inoculated with potassium thiocyanate extract of Pasteurella multocida type A. Am J Vet Res. 1978 Aug;39(8):1269–1273. [PubMed] [Google Scholar]
  21. Mukkur T. K., Pyliotis N. A., Barnes A. Possible immunological synergism among the protective antigens of Pasteurella multocida. type A. J Comp Pathol. 1982 Apr;92(2):249–260. doi: 10.1016/0021-9975(82)90083-4. [DOI] [PubMed] [Google Scholar]
  22. Mukkur T. K., Pyliotis N. A. The potassium thiocyanate extract of Pasteurella multocida: electron microscopy and susceptibility of its immunogenic activity to some physical, chemical and enzymatic treatments. J Comp Pathol. 1981 Jul;91(3):427–437. doi: 10.1016/0021-9975(81)90013-x. [DOI] [PubMed] [Google Scholar]
  23. Percy D. H., Prescott J. F., Bhasin J. L. Pasteurella multocida infection in the domestic rabbit: immunization with a streptomycin-dependent mutant. Can J Comp Med. 1985 Apr;49(2):227–230. [PMC free article] [PubMed] [Google Scholar]
  24. Rimler R. B., Brogden K. A. Pasteurella multocida isolated from rabbits and swine: serologic types and toxin production. Am J Vet Res. 1986 Apr;47(4):730–737. [PubMed] [Google Scholar]
  25. Ringler D. H., Peter G. K., Chrisp C. E., Keren D. F. Protection of rabbits against experimental pasteurellosis by vaccination with a potassium thiocyanate extract of Pasteurella multocida. Infect Immun. 1985 Sep;49(3):498–504. doi: 10.1128/iai.49.3.498-504.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Rutter J. M. Virulence of Pasteurella multocida in atrophic rhinitis of gnotobiotic pigs infected with Bordetella bronchiseptica. Res Vet Sci. 1983 May;34(3):287–295. [PubMed] [Google Scholar]
  27. Tsai C. M., Frasch C. E. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal Biochem. 1982 Jan 1;119(1):115–119. doi: 10.1016/0003-2697(82)90673-x. [DOI] [PubMed] [Google Scholar]
  28. Weisbroth S. H., Scher S. The establishment of a specific-pathogen-free rabbit breeding colony. II. Monitoring for disease and health statistics. Lab Anim Care. 1969 Dec;19(6):795–799. [PubMed] [Google Scholar]
  29. Welch W. D., Lu Y. S., Bawdon R. E. Pharmacokinetics of penicillin-G in serum and nasal washings of Pasteurella multocida free and infected rabbits. Lab Anim Sci. 1987 Feb;37(1):65–68. [PubMed] [Google Scholar]
  30. Williams R. C., Gibbons R. J. Inhibition of bacterial adherence by secretory immunoglobulin A: a mechanism of antigen disposal. Science. 1972 Aug 25;177(4050):697–699. doi: 10.1126/science.177.4050.697. [DOI] [PubMed] [Google Scholar]

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