Abstract
Mononuclear phagocytes may be important effector cells against Giardia lamblia. Human monocyte-derived macrophages were incubated with G. lamblia trophozoites in 13% heat-inactivated autologous serum. At a G. lamblia/macrophage ratio of 1:1, the number of trophozoites ingested per 100 macrophages ranged from 1 to 12 at 0.5 h and increased for all donors (n = 6) to 10 to 92 at 8 h. Ingestion was confirmed by electron microscopy. Increasing the parasite/phagocyte ratio to 5:1 increased the percentage of macrophages with adherent but not ingested trophozoites. Incubating Giardia cells and macrophages with 20% immune serum increased ingestion of parasites eightfold, indicating that anti-G. lamblia antibody can enhance ingestion. Both phase-contrast microscopy and electron microscopy documented trophozoite destruction within macrophages. Ingestion of parasites elicited an oxidative burst as measured by luminol-enhanced chemiluminescence. In vitro, Giardia trophozoites were killed by greater than or equal to 5 X 10(-5) M H2O2. Fusion of lysosomes with parasite-containing phagosomes was suggested by acridine orange-stained preparations. Human macrophages have the capacity to ingest Giardia trophozoites and to kill intracellular parasites, possibly by oxidative microbicidal mechanisms.
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Selected References
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- Aggarwal A., Nash T. E. Lack of cellular cytotoxicity by human mononuclear cells to Giardia. J Immunol. 1986 May 1;136(9):3486–3488. [PubMed] [Google Scholar]
- Andrews J. S., Jr, Hewlett E. L. Protection against infection with Giardia muris by milk containing antibody to Giardia. J Infect Dis. 1981 Feb;143(2):242–246. doi: 10.1093/infdis/143.2.242. [DOI] [PubMed] [Google Scholar]
- Duncombe V. M., Bolin T. D., Davis A. E., Cummins A. G., Crouch R. L. Histopathology in giardiasis: a correlation with diarrhoea. Aust N Z J Med. 1978 Aug;8(4):392–396. doi: 10.1111/j.1445-5994.1978.tb04908.x. [DOI] [PubMed] [Google Scholar]
- Elsbach P. Degradation of microorganisms by phagocytic cells. Rev Infect Dis. 1980 Jan-Feb;2(1):106–128. doi: 10.1093/clinids/2.1.106. [DOI] [PubMed] [Google Scholar]
- Elson C. O., Heck J. A., Strober W. T-cell regulation of murine IgA synthesis. J Exp Med. 1979 Mar 1;149(3):632–643. doi: 10.1084/jem.149.3.632. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hart P. D., Young M. R. Interference with normal phagosome-lysosome fusion in macrophages, using ingested yeast cells and suramin. Nature. 1975 Jul 3;256(5512):47–49. doi: 10.1038/256047a0. [DOI] [PubMed] [Google Scholar]
- Heyworth M. F. Antibody response to Giardia muris trophozoites in mouse intestine. Infect Immun. 1986 May;52(2):568–571. doi: 10.1128/iai.52.2.568-571.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heyworth M. F., Owen R. L., Jones A. L. Comparison of leukocytes obtained from the intestinal lumen of Giardia-infected immunocompetent mice and nude mice. Gastroenterology. 1985 Dec;89(6):1360–1365. doi: 10.1016/0016-5085(85)90656-0. [DOI] [PubMed] [Google Scholar]
- Hill D. R., Burge J. J., Pearson R. D. Susceptibility of Giardia lamblia trophozoites to the lethal effect of human serum. J Immunol. 1984 Apr;132(4):2046–2052. [PubMed] [Google Scholar]
- Hill D. R., Pohl R., Pearson R. D. Giardia lamblia: a culture method for determining parasite viability. Am J Trop Med Hyg. 1986 Nov;35(6):1129–1133. doi: 10.4269/ajtmh.1986.35.1129. [DOI] [PubMed] [Google Scholar]
- Istre G. R., Dunlop T. S., Gaspard G. B., Hopkins R. S. Waterborne giardiasis at a mountain resort: evidence for acquired immunity. Am J Public Health. 1984 Jun;74(6):602–604. doi: 10.2105/ajph.74.6.602. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kaplan B. S., Uni S., Aikawa M., Mahmoud A. A. Effector mechanism of host resistance in murine giardiasis: specific IgG and IgA cell-mediated toxicity. J Immunol. 1985 Mar;134(3):1975–1981. [PubMed] [Google Scholar]
- Knight R. Epidemiology and transmission of giardiasis. Trans R Soc Trop Med Hyg. 1980;74(4):433–436. doi: 10.1016/0035-9203(80)90044-9. [DOI] [PubMed] [Google Scholar]
- LeFevre M. E., Hammer R., Joel D. D. Macrophages of the mammalian small intestine: a review. J Reticuloendothel Soc. 1979 Nov;26(5):553–573. [PubMed] [Google Scholar]
- Meshnick S. R., Eaton J. W. Leishmanial superoxide dismutase: a possible target for chemotherapy. Biochem Biophys Res Commun. 1981 Oct 15;102(3):970–976. doi: 10.1016/0006-291x(81)91633-8. [DOI] [PubMed] [Google Scholar]
- Meyer E. A. Giardia lamblia: isolation and axenic cultivation. Exp Parasitol. 1976 Feb;39(1):101–105. doi: 10.1016/0014-4894(76)90016-3. [DOI] [PubMed] [Google Scholar]
- Murray H. W., Cohn Z. A. Macrophage oxygen-dependent antimicrobial activity. I. Susceptibility of Toxoplasma gondii to oxygen intermediates. J Exp Med. 1979 Oct 1;150(4):938–949. doi: 10.1084/jem.150.4.938. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nakagawara A., Nathan C. F., Cohn Z. A. Hydrogen peroxide metabolism in human monocytes during differentiation in vitro. J Clin Invest. 1981 Nov;68(5):1243–1252. doi: 10.1172/JCI110370. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nathan C. F., Murray H. W., Cohn Z. A. The macrophage as an effector cell. N Engl J Med. 1980 Sep 11;303(11):622–626. doi: 10.1056/NEJM198009113031106. [DOI] [PubMed] [Google Scholar]
- Nathan C., Nogueira N., Juangbhanich C., Ellis J., Cohn Z. Activation of macrophages in vivo and in vitro. Correlation between hydrogen peroxide release and killing of Trypanosoma cruzi. J Exp Med. 1979 May 1;149(5):1056–1068. doi: 10.1084/jem.149.5.1056. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Owen R. L., Allen C. L., Stevens D. P. Phagocytosis of Giardia muris by macrophages in Peyer's patch epithelium in mice. Infect Immun. 1981 Aug;33(2):591–601. doi: 10.1128/iai.33.2.591-601.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Oyerinde J. P., Ogunbi O., Alonge A. A. Age and sex distribution of infections with Entamoeba histolytica and Giardia intestinalis in the Lagos population. Int J Epidemiol. 1977 Sep;6(3):231–234. doi: 10.1093/ije/6.3.231. [DOI] [PubMed] [Google Scholar]
- Pearson R. D., Steigbigel R. T. Phagocytosis and killing of the protozoan Leishmania donovani by human polymorphonuclear leukocytes. J Immunol. 1981 Oct;127(4):1438–1443. [PubMed] [Google Scholar]
- Radulescu S., Meyer E. A. Opsonization in vitro of Giardia lamblia trophozoites. Infect Immun. 1981 May;32(2):852–856. doi: 10.1128/iai.32.2.852-856.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Roberts-Thomson I. C., Mitchell G. F. Giardiasis in mice. I. Prolonged infections in certain mouse strains and hypothymic (nude) mice. Gastroenterology. 1978 Jul;75(1):42–46. [PubMed] [Google Scholar]
- Roberts-Thomson I. C., Stevens D. P., Mahmoud A. A., Warren K. S. Acquired resistance to infection in an animal model of giardiasis. J Immunol. 1976 Nov;117(5 PT2):2036–2037. [PubMed] [Google Scholar]
- Saha T. K., Ghosh T. K. Invasion of small intestinal mucosa by Giardia lamblia in man. Gastroenterology. 1977 Mar;72(3):402–405. [PubMed] [Google Scholar]
- Smith P. D., Elson C. O., Keister D. B., Nash T. E. Human host response to Giardia lamblia. I. Spontaneous killing by mononuclear leukocytes in vitro. J Immunol. 1982 Mar;128(3):1372–1376. [PubMed] [Google Scholar]
- Snider D. P., Underdown B. J. Quantitative and temporal analyses of murine antibody response in serum and gut secretions to infection with Giardia muris. Infect Immun. 1986 Apr;52(1):271–278. doi: 10.1128/iai.52.1.271-278.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stossel T. P. Phagocytosis: recognition and ingestion. Semin Hematol. 1975 Jan;12(1):83–116. [PubMed] [Google Scholar]
- Unanue E. R. Cooperation between mononuclear phagocytes and lymphocytes in immunity. N Engl J Med. 1980 Oct 23;303(17):977–985. doi: 10.1056/NEJM198010233031706. [DOI] [PubMed] [Google Scholar]
- Visvesvara G. S. Axenic growth of Giardia lamblia in Diamond's TPS-1 medium. Trans R Soc Trop Med Hyg. 1980;74(2):213–215. doi: 10.1016/0035-9203(80)90249-7. [DOI] [PubMed] [Google Scholar]
- Visvesvara G. S., Smith P. D., Healy G. R., Brown W. R. An immunofluorescence test to detect serum antibodies to Giardia lamblia. Ann Intern Med. 1980 Dec;93(6):802–805. doi: 10.7326/0003-4819-93-6-802. [DOI] [PubMed] [Google Scholar]
- Yam L. T., Li C. Y., Crosby W. H. Cytochemical identification of monocytes and granulocytes. Am J Clin Pathol. 1971 Mar;55(3):283–290. doi: 10.1093/ajcp/55.3.283. [DOI] [PubMed] [Google Scholar]