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. 1987 Dec;55(12):3213–3214. doi: 10.1128/iai.55.12.3213-3214.1987

Characterization of the secreted antigens of Mycobacterium bovis BCG: comparison of the 46-kilodalton dimeric protein with proteins MPB64 and MPB70.

C Abou-Zeid 1, M Harboe 1, G A Rook 1
PMCID: PMC260053  PMID: 3316036

Abstract

Western blot analysis showed that the 46-kilodalton (kDa) dimeric protein antigen secreted in large amounts by some daughter strains of Mycobacterium bovis BCG corresponded to protein MPB70 present in long-term culture filtrates of the Japanese substrain. The 46/23-kDa antigen is the most abundant protein in supernatant from a 5-day culture but is masked by leaked products in old culture supernatants. No similarities were found between the 46-kDa protein and MPB64, a protein with the same strain distribution, or with the antigen of similar molecular mass recognized by monoclonal antibody SA1.D2D.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abou-Zeid C., Filley E., Steele J., Rook G. A. A simple new method for using antigens separated by polyacrylamide gel electrophoresis to stimulate lymphocytes in vitro after converting bands cut from Western blots into antigen-bearing particles. J Immunol Methods. 1987 Apr 2;98(1):5–10. doi: 10.1016/0022-1759(87)90429-7. [DOI] [PubMed] [Google Scholar]
  2. Abou-Zeid C., Smith I., Grange J., Steele J., Rook G. Subdivision of daughter strains of bacille Calmette-Guérin (BCG) according to secreted protein patterns. J Gen Microbiol. 1986 Nov;132(11):3047–3053. doi: 10.1099/00221287-132-11-3047. [DOI] [PubMed] [Google Scholar]
  3. Harboe M., Nagai S. MPB70, a unique antigen of Mycobacterium bovis BCG. Am Rev Respir Dis. 1984 Mar;129(3):444–452. doi: 10.1164/arrd.1984.129.3.444. [DOI] [PubMed] [Google Scholar]
  4. Harboe M., Nagai S., Patarroyo M. E., Torres M. L., Ramirez C., Cruz N. Properties of proteins MPB64, MPB70, and MPB80 of Mycobacterium bovis BCG. Infect Immun. 1986 Apr;52(1):293–302. doi: 10.1128/iai.52.1.293-302.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Miura K., Nagai S., Kinomoto M., Haga S., Tokunaga T. Comparative studies with various substrains of Mycobacterium bovis BCG on the production of an antigenic protein, MPB70. Infect Immun. 1983 Feb;39(2):540–545. doi: 10.1128/iai.39.2.540-545.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Nagai S., Matsumoto J., Nagasuga T. Specific skin-reactive protein from culture filtrate of Mycobacterium bovis BCG. Infect Immun. 1981 Mar;31(3):1152–1160. doi: 10.1128/iai.31.3.1152-1160.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Young D. B., Fohn M. J., Khanolkar S. R., Buchanan T. M. Monoclonal antibodies to a 28,000 mol. wt protein antigen of Mycobacterium leprae. Clin Exp Immunol. 1985 Jun;60(3):546–552. [PMC free article] [PubMed] [Google Scholar]

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