Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1986 Jul;53(1):99–102. doi: 10.1128/iai.53.1.99-102.1986

Lectinlike adhesins in the Bacteroides fragilis group.

V Rogemond, R M Guinet
PMCID: PMC260081  PMID: 3721582

Abstract

Lectinlike adhesins were identified in the Bacteroides fragilis group by using sugars immobilized on agarose beads either with whole bacteria by direct microscopic examination or with soluble extracts by immunoaffinoelectrophoresis. These two methods allowed the identification of two sugars reacting with whole bacteria and with the corresponding extracts: alpha-D-glucosamine and D-galactosamine. Among eight strains tested representing seven species, the two strains of B. fragilis were equally adhesive and showed the greatest adhesions. The lectinlike adhesin was purified by affinity chromatography on glucosamine-agarose or galactosamine-agarose and showed one band at 70,000 daltons in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. This lectinlike adhesin may help to elucidate the roles of the B. fragilis group in the colonization of intestinal surfaces and in the predominance of B. fragilis in infections alone and in synergy with other bacteria.

Full text

PDF
99

Images in this article

100Image
on p.100
101Image
on p.101
101Image
on p.101
102Image
on p.102

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barondes S. H. Soluble lectins: a new class of extracellular proteins. Science. 1984 Mar 23;223(4642):1259–1264. doi: 10.1126/science.6367039. [DOI] [PubMed] [Google Scholar]
  2. Boyd J., McBride B. C. Fractionation of hemagglutinating and bacterial binding adhesins of Bacteroides gingivalis. Infect Immun. 1984 Aug;45(2):403–409. doi: 10.1128/iai.45.2.403-409.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hofstad T. Pathogenicity of anaerobic gram-negative rods: possible mechanisms. Rev Infect Dis. 1984 Mar-Apr;6(2):189–199. doi: 10.1093/clinids/6.2.189. [DOI] [PubMed] [Google Scholar]
  4. Kasper D. L., Hayes M. E., Reinap B. G., Craft F. O., Onderdonk A. B., Polk B. F. Isolation and identification of encapsulated strains of Bacteroides fragilis. J Infect Dis. 1977 Jul;136(1):75–81. doi: 10.1093/infdis/136.1.75. [DOI] [PubMed] [Google Scholar]
  5. Kroll J. Rocket-line immunoelectrophoresis (76). Scand J Immunol Suppl. 1973;1:83–87. [PubMed] [Google Scholar]
  6. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  7. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  8. Merril C. R., Goldman D., Sedman S. A., Ebert M. H. Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins. Science. 1981 Mar 27;211(4489):1437–1438. doi: 10.1126/science.6162199. [DOI] [PubMed] [Google Scholar]
  9. Moore W. E., Cato E. P., Holdeman L. V. Anaerobic bacteria of the gastrointestinal flora and their occurrence in clinical infections. J Infect Dis. 1969 Jun;119(6):641–649. doi: 10.1093/infdis/119.6.641. [DOI] [PubMed] [Google Scholar]
  10. Moore W. E., Holdeman L. V. Human fecal flora: the normal flora of 20 Japanese-Hawaiians. Appl Microbiol. 1974 May;27(5):961–979. doi: 10.1128/am.27.5.961-979.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Okuda K., Takazoe I. Haemagglutinating activity of Bacteroides melaninogenicus. Arch Oral Biol. 1974 May;19(5):415–416. doi: 10.1016/0003-9969(74)90184-8. [DOI] [PubMed] [Google Scholar]
  12. Onderdonk A. B., Kasper D. L., Cisneros R. L., Bartlett J. G. The capsular polysaccharide of Bacteroides fragilis as a virulence factor: comparison of the pathogenic potential of encapsulated and unencapsulated strains. J Infect Dis. 1977 Jul;136(1):82–89. doi: 10.1093/infdis/136.1.82. [DOI] [PubMed] [Google Scholar]
  13. Rotstein O. D., Pruett T. L., Simmons R. L. Mechanisms of microbial synergy in polymicrobial surgical infections. Rev Infect Dis. 1985 Mar-Apr;7(2):151–170. doi: 10.1093/clinids/7.2.151. [DOI] [PubMed] [Google Scholar]
  14. Slots J., Gibbons R. J. Attachment of Bacteroides melaninogenicus subsp. asaccharolyticus to oral surfaces and its possible role in colonization of the mouth and of periodontal pockets. Infect Immun. 1978 Jan;19(1):254–264. doi: 10.1128/iai.19.1.254-264.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Weeke B. Crossed immunoelectrophoresis. Scand J Immunol Suppl. 1973;1:47–56. doi: 10.1111/j.1365-3083.1973.tb03778.x. [DOI] [PubMed] [Google Scholar]
  16. West S. E., Wilkins T. D. Vaspar broth-disk procedure for antibiotic susceptibility testing of anaerobic bacteria. Antimicrob Agents Chemother. 1980 Feb;17(2):288–291. doi: 10.1128/aac.17.2.288. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wren M. W., Baldwin A. W., Eldon C. P., Sanderson P. J. The anaerobic culture of clinical specimens: a 14-month study. J Med Microbiol. 1977 Feb;10(1):49–61. doi: 10.1099/00222615-10-1-49. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES