Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1986 Jul;53(1):161–165. doi: 10.1128/iai.53.1.161-165.1986

Killing of Actinobacillus actinomycetemcomitans by the human neutrophil myeloperoxidase-hydrogen peroxide-chloride system.

K T Miyasaki, M E Wilson, R J Genco
PMCID: PMC260091  PMID: 3013779

Abstract

Actinobacillus actinomycetemcomitans is a facultative gram-negative coccobacillus associated with periodontal disease and nonoral infections. This organism is resistant to serum bactericidal mechanisms but is nevertheless killed by human neutrophils under aerobic and anaerobic conditions. Most of the killing attributable to oxidative mechanisms is inhibited by sodium cyanide, which suggests that the myeloperoxidase-hydrogen peroxide-chloride (MPO-H2O2-Cl-) system may be a key factor in the oxidative killing process. In this report, we examine whether the isolated MPO-H2O2-Cl- system is bactericidal against A. actinomycetemcomitans. We found that three major chromatographic forms of MPO were capable of killing A. actinomycetemcomitans at sublethal concentrations of H2O2 and that both catalase-positive and catalase-negative strains of this organism were sensitive to killing by the MPO-H2O2-Cl- system. We conclude that the isolated MPO-H2O2-Cl- system is bactericidal for A. actinomycetemcomitans independent of other neutrophil granule constituents and may be an important component of the oxygen-dependent bactericidal activity of the neutrophil with respect to this periodontopathic organism.

Full text

PDF
161

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cainciola L. J., Genco R. J., Patters M. R., McKenna J., van Oss C. J. Defective polymorphonuclear leukocyte function in a human periodontal disease. Nature. 1977 Feb 3;265(5593):445–447. doi: 10.1038/265445a0. [DOI] [PubMed] [Google Scholar]
  2. Cech P., Lehrer R. I. Phagolysosomal pH of human neutrophils. Blood. 1984 Jan;63(1):88–95. [PubMed] [Google Scholar]
  3. Clark R. A., Page R. C., Wilde G. Defective neutrophil chemotaxis in juvenile periodontitis. Infect Immun. 1977 Dec;18(3):694–700. doi: 10.1128/iai.18.3.694-700.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Klebanoff S. J. Myeloperoxidase: contribution to the microbicidal activity of intact leukocytes. Science. 1970 Sep 11;169(3950):1095–1097. doi: 10.1126/science.169.3950.1095. [DOI] [PubMed] [Google Scholar]
  5. Lavine W. S., Maderazo E. G., Stolman J., Ward P. A., Cogen R. B., Greenblatt I., Robertson P. B. Impaired neutrophil chemotaxis in patients with juvenile and rapidly progressing periodontitis. J Periodontal Res. 1979 Jan;14(1):10–19. doi: 10.1111/j.1600-0765.1979.tb00213.x. [DOI] [PubMed] [Google Scholar]
  6. Miyasaki K. T., Wilson M. E., Brunetti A. J., Genco R. J. Oxidative and nonoxidative killing of Actinobacillus actinomycetemcomitans by human neutrophils. Infect Immun. 1986 Jul;53(1):154–160. doi: 10.1128/iai.53.1.154-160.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Miyasaki K. T., Wilson M. E., Cohen E., Jones P. C., Genco R. J. Evidence for and partial characterization of three major and three minor chromatographic forms of human neutrophil myeloperoxidase. Arch Biochem Biophys. 1986 May 1;246(2):751–764. doi: 10.1016/0003-9861(86)90332-2. [DOI] [PubMed] [Google Scholar]
  8. Miyasaki K. T., Wilson M. E., Reynolds H. S., Genco R. J. Resistance of Actinobacillus actinomycetemcomitans and differential susceptibility of oral Haemophilus species to the bactericidal effects of hydrogen peroxide. Infect Immun. 1984 Dec;46(3):644–648. doi: 10.1128/iai.46.3.644-648.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Miyasaki K. T., Wilson M. E., Zambon J. J., Genco R. J. Influence of endogenous catalase activity on the sensitivity of the oral bacterium Actinobacillus actinomycetemcomitans and the oral haemophili to the bactericidal properties of hydrogen peroxide. Arch Oral Biol. 1985;30(11-12):843–848. doi: 10.1016/0003-9969(85)90141-4. [DOI] [PubMed] [Google Scholar]
  10. Odeberg H., Olsson I. Microbicidal mechanisms of human granulocytes: synergistic effects of granulocyte elastase and myeloperoxidase or chymotrypsin-like cationic protein. Infect Immun. 1976 Dec;14(6):1276–1283. doi: 10.1128/iai.14.6.1276-1283.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Pember S. O., Shapira R., Kinkade J. M., Jr Multiple forms of myeloperoxidase from human neutrophilic granulocytes: evidence for differences in compartmentalization, enzymatic activity, and subunit structure. Arch Biochem Biophys. 1983 Mar;221(2):391–403. doi: 10.1016/0003-9861(83)90158-3. [DOI] [PubMed] [Google Scholar]
  12. Rest R. F., Spitznagel J. K. Myeloperoxidase-Cl--H2O2 bactericidal system: effect of bacterial membrane structure and growth conditions. Infect Immun. 1978 Mar;19(3):1110–1112. doi: 10.1128/iai.19.3.1110-1112.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Selvaraj R. J., Paul B. B., Strauss R. R., Jacobs A. A., Sbarra A. J. Oxidative peptide cleavage and decarboxylation by the MPO-H2O2-Cl- antimicrobial system. Infect Immun. 1974 Feb;9(2):255–260. doi: 10.1128/iai.9.2.255-260.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Selvaraj R. J., Zgliczynski J. M., Paul B. B., Sbarra A. J. Enhanced killing of myeloperoxidase-coated bacteria in the myeloperoxidase-H2O2-Cl- system. J Infect Dis. 1978 Apr;137(4):481–485. doi: 10.1093/infdis/137.4.481. [DOI] [PubMed] [Google Scholar]
  15. Sips H. J., Hamers M. N. Mechanism of the bactericidal action of myeloperoxidase: increased permeability of the Escherichia coli cell envelope. Infect Immun. 1981 Jan;31(1):11–16. doi: 10.1128/iai.31.1.11-16.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Slots J., Reynolds H. S., Genco R. J. Actinobacillus actinomycetemcomitans in human periodontal disease: a cross-sectional microbiological investigation. Infect Immun. 1980 Sep;29(3):1013–1020. doi: 10.1128/iai.29.3.1013-1020.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Slots J. The predominant cultivable organisms in juvenile periodontitis. Scand J Dent Res. 1976 Jan;84(1):1–10. doi: 10.1111/j.1600-0722.1976.tb00454.x. [DOI] [PubMed] [Google Scholar]
  18. Stelmaszyńska T., Zgliczynski J. M. N-(2-Oxoacyl)amino acids and nitriles as final products of dipeptide chlorination mediated by the myeloperoxidase/H2O2/Cl- system. Eur J Biochem. 1978 Dec 1;92(1):301–308. doi: 10.1111/j.1432-1033.1978.tb12748.x. [DOI] [PubMed] [Google Scholar]
  19. Sundqvist G., Johansson E. Bactericidal effect of pooled human serum on Bacteroides melaninogenicus, Bacteroides asaccharolyticus and Actinobacillus actinomycetemcomitans. Scand J Dent Res. 1982 Feb;90(1):29–36. doi: 10.1111/j.1600-0722.1982.tb01521.x. [DOI] [PubMed] [Google Scholar]
  20. Weiss S. J., Peppin G., Ortiz X., Ragsdale C., Test S. T. Oxidative autoactivation of latent collagenase by human neutrophils. Science. 1985 Feb 15;227(4688):747–749. doi: 10.1126/science.2982211. [DOI] [PubMed] [Google Scholar]
  21. Wright C. D., Bowie J. U., Gray G. R., Nelson R. D. Candidacidal activity of myeloperoxidase: mechanisms of inhibitory influence of soluble cell wall mannan. Infect Immun. 1983 Oct;42(1):76–80. doi: 10.1128/iai.42.1.76-80.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Yamada M., Mori M., Sugimura T. Purification and characterization of small molecular weight myeloperoxidase from human promyelocytic leukemia HL-60 cells. Biochemistry. 1981 Feb 17;20(4):766–771. doi: 10.1021/bi00507a018. [DOI] [PubMed] [Google Scholar]
  23. Zambon J. J. Actinobacillus actinomycetemcomitans in human periodontal disease. J Clin Periodontol. 1985 Jan;12(1):1–20. doi: 10.1111/j.1600-051x.1985.tb01348.x. [DOI] [PubMed] [Google Scholar]
  24. Zambon J. J., Slots J., Genco R. J. Serology of oral Actinobacillus actinomycetemcomitans and serotype distribution in human periodontal disease. Infect Immun. 1983 Jul;41(1):19–27. doi: 10.1128/iai.41.1.19-27.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Zgliczynski J. M., Selvaraj R. J., Paul B. B., Stelmaszynska T., Poskitt P. K., Sbarra A. J. Chlorination by the myeloperoxidase-H2O2-Cl- antimicrobial system at acid and neutral pH. Proc Soc Exp Biol Med. 1977 Mar;154(3):418–422. doi: 10.3181/00379727-154-39684. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES