TABLE 3.

Kar9p-L304P displays a synthetic lethal phenotype in combination with dynein mutants

Cross	Viability	Tetrads (PD:TT:NPD)	Double mutants viable/predicted
kar9-L304P × dyn1Δ	Not viable	25 (6:15:4)	0/23
kar9-L304P × jnm1Δ	Not viable	24 (5:15:4)	0/23
kar9-L304P × kip2Δ	Synthetic sick	22 (4:17:1)	0/19
kar9-L304P × bim1Δ	Viable	31 (6:23:2)	27/27

Double mutants were generated by meiotic crosses and scored for growth after 2–3 days. Progeny exhibiting no growth or forming microcolonies ∼10-fold smaller than single mutant or wild-type cells (see Figure 9) were scored as “not viable.” The kar9-L304P kip2Δ double mutants were scored as “synthetic sick” as these colonies were smaller than those produced by single mutants but did not exhibit phenotypes as severe as those observed for other double mutants. The following parental strains were used in these crosses: kar9-L304P (yRM6739 or yRM6740), dyn1Δ (yRM425), jnm1Δ (yRM6398), kip2Δ (yRM435), and bim1Δ (yRM6764).