Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1986 Nov;54(2):371–378. doi: 10.1128/iai.54.2.371-378.1986

Effect of rat surfactant lipids on complement and Fc receptors of macrophages.

J D Coonrod, M C Jarrells, K Yoneda
PMCID: PMC260171  PMID: 3770948

Abstract

Murine resident alveolar macrophages have very low numbers of complement receptors detectable by rosetting with erythrocyte complexes. These macrophages are relatively inactive in tests of complement- or antibody-mediated bacterial phagocytosis in vitro. It is not known whether these characteristics are intrinsic or are environmentally modulated. We found previously that rat bronchoalveolar lavage fluid or isolated rat surfactant lipid causes a unique and marked reduction in the ability of peritoneal macrophages to form rosettes with immunoglobulin G or complement containing erythrocyte complexes in vitro. In this study the antirosetting activity of rat surfactant was found to be due to its neutral lipid component and, specifically, to the free fatty acid (FFA) fraction of the neutral lipids. Rat surfactant contained a higher level of FFA than has been reported for canine, guinea pig, or human surfactant. Studies with pure FFA showed that activity in blocking macrophage Fc and complement receptors correlated with increasing unsaturation and chain length. A mixture of eight commercially available FFAs, representing the most abundant FFA in rat surfactant and consisting mostly of saturated FFA, had much less effect on rat macrophage receptors than the naturally occurring FFA mixture. These findings suggest that long-chain-unsaturated FFAs in rat surfactant are the most important for the antireceptor activity. The antireceptor activity of rat surfactant or FFA on peritoneal macrophage receptors in vitro and known intrinsic properties of murine alveolar macrophages could not be precisely correlated, suggesting that impaired binding of immune complexes by murine alveolar macrophages and a high level of FFAs in rat surfactant may be independent rather than causally related phenomena.

Full text

PDF
371

Images in this article

376Image
on p.376

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  2. Badwey J. A., Curnutte J. T., Karnovsky M. L. cis-Polyunsaturated fatty acids induce high levels of superoxide production by human neutrophils. J Biol Chem. 1981 Dec 25;256(24):12640–12643. [PubMed] [Google Scholar]
  3. Chait A., Iverius P. H., Brunzell J. D. Lipoprotein lipase secretion by human monocyte-derived macrophages. J Clin Invest. 1982 Feb;69(2):490–493. doi: 10.1172/JCI110473. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chandler D. B., Fuller W. C., Jackson R. M., Fulmer J. D. Studies of membrane receptors and phagocytosis in subpopulations of rat alveolar macrophages. Am Rev Respir Dis. 1986 Mar;133(3):461–467. doi: 10.1164/arrd.1986.133.3.461. [DOI] [PubMed] [Google Scholar]
  5. Coonrod J. D., Lester R. L., Hsu L. C. Characterization of the extracellular bactericidal factors of rat alveolar lining material. J Clin Invest. 1984 Oct;74(4):1269–1279. doi: 10.1172/JCI111537. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Coonrod J. D., Rehm S. R. Complement receptors of rat macrophages. J Reticuloendothel Soc. 1982 Feb;31(2):107–115. [PubMed] [Google Scholar]
  7. Coonrod J. D., Yoneda K. Complement and opsonins in alveolar secretions and serum of rats with pneumonia due to Streptococcus pneumoniae. Rev Infect Dis. 1981 Mar-Apr;3(2):310–322. doi: 10.1093/clinids/3.2.310. [DOI] [PubMed] [Google Scholar]
  8. Coonrod J. D., Yoneda K. Detection and partial characterization of antibacterial factor(s) in alveolar lining material of rats. J Clin Invest. 1983 Jan;71(1):129–141. doi: 10.1172/JCI110741. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Coonrod J. D., Yoneda K. Effect of rat alveolar lining material on macrophage receptors. J Immunol. 1983 Jun;130(6):2589–2596. [PubMed] [Google Scholar]
  10. Finley T. N., Ladman A. J. Low yield of pulmonary surfactant in cigarette smokers. N Engl J Med. 1972 Feb 3;286(5):223–227. doi: 10.1056/NEJM197202032860501. [DOI] [PubMed] [Google Scholar]
  11. Hearst J. E., Warr G. A., Jakab G. J. Characterization of murine lung and peritoneal macrophages. J Reticuloendothel Soc. 1980 May;27(5):443–454. [PubMed] [Google Scholar]
  12. Karnovsky M. J., Kleinfeld A. M., Hoover R. L., Klausner R. D. The concept of lipid domains in membranes. J Cell Biol. 1982 Jul;94(1):1–6. doi: 10.1083/jcb.94.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kelly J. P., Parker C. W. Effects of arachidonic acid and other unsaturated fatty acids on mitogenesis in human lymphocytes. J Immunol. 1979 Apr;122(4):1556–1562. [PubMed] [Google Scholar]
  14. Khoo J. C., Mahoney E. M., Witztum J. L. Secretion of lipoprotein lipase by macrophages in culture. J Biol Chem. 1981 Jul 25;256(14):7105–7108. [PubMed] [Google Scholar]
  15. King R. J., Clements J. A. Surface active materials from dog lung. II. Composition and physiological correlations. Am J Physiol. 1972 Sep;223(3):715–726. doi: 10.1152/ajplegacy.1972.223.3.715. [DOI] [PubMed] [Google Scholar]
  16. Kradin R. L., McCarthy K. M., Schneeberger E. E. Opsonic receptor function is reduced on the surface of newborn alveolar macrophages. Am Rev Respir Dis. 1986 Feb;133(2):238–244. doi: 10.1164/arrd.1986.133.2.238. [DOI] [PubMed] [Google Scholar]
  17. Newman S. L., Musson R. A., Henson P. M. Development of functional complement receptors during in vitro maturation of human monocytes into macrophages. J Immunol. 1980 Nov;125(5):2236–2244. [PubMed] [Google Scholar]
  18. Nguyen B. Y., Peterson P. K., Verbrugh H. A., Quie P. G., Hoidal J. R. Differences in phagocytosis and killing by alveolar macrophages from humans, rabbits, rats, and hamsters. Infect Immun. 1982 May;36(2):504–509. doi: 10.1128/iai.36.2.504-509.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Pfleger R. C., Thomas H. G. Beagle dog pulmonary surfactant lipids. Lipid composition of pulmonary tissue, exfoliated lining cells and surfactant. Arch Intern Med. 1971 May;127(5):863–872. doi: 10.1001/archinte.127.5.863. [DOI] [PubMed] [Google Scholar]
  20. Pjura W. J., Kleinfeld A. M., Klausner R. D., Karnovsky M. J. Fatty Acid perturbation of a membrane protein-lipid interaction: a terbium (tb) study. Biophys J. 1982 Jan;37(1):69–71. doi: 10.1016/S0006-3495(82)84604-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sahu S., Lynn W. S. Phospholipase A in pulmonary secretions of patients with alveolar proteinosis. Biochim Biophys Acta. 1977 May 25;487(2):354–360. doi: 10.1016/0005-2760(77)90011-x. [DOI] [PubMed] [Google Scholar]
  22. Warr G. A., Jakab G. J., Hearst J. E. Alterations in lung macrophage immune receptor(s) activity associated with viral pneumonia. J Reticuloendothel Soc. 1979 Oct;26(4):357–366. [PubMed] [Google Scholar]
  23. Weinberg D. S., Unanue E. R. Antigen-presenting function of alveolar macrophages: uptake and presentation of Listeria monocytogenes. J Immunol. 1981 Feb;126(2):794–799. [PubMed] [Google Scholar]
  24. van oud Alblas A. B., van Furth R. Origin, Kinetics, and characteristics of pulmonary macrophages in the normal steady state. J Exp Med. 1979 Jun 1;149(6):1504–1518. doi: 10.1084/jem.149.6.1504. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES