Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1986 Dec;54(3):774–779. doi: 10.1128/iai.54.3.774-779.1986

Antigenic characterization of the P6 protein of nontypable Haemophilus influenzae.

T F Murphy, L C Bartos, A A Campagnari, M B Nelson, M A Apicella
PMCID: PMC260236  PMID: 3491049

Abstract

The purpose of this study was to characterize the degree of antigenic heterogeneity or conservation of a 16,600-dalton outer membrane protein (P6) among strains of nontypable Haemophilus influenzae. Immunization of rabbits with P6 isolated from individual strains resulted in antibody to P6 of all 25 strains tested. The titers of antibody in the sera were similar among the strains. Whole organisms of two strains were used to immunize rabbits, and antibodies were produced to P6 of all strains tested. Monoclonal antibodies developed to P6 from mice immunized with whole cells of three different strains recognized determinants on P6 of all 25 strains tested. Finally, pooled normal human serum contained antibodies to P6 of all 25 strains assayed. These studies indicate that P6 is a highly conserved antigen on the outer membrane of nontypable H. influenzae.

Full text

PDF
774

Images in this article

776Image
on p.776
776Image
on p.776
777Image
on p.777
777Image
on p.777
778Image
on p.778

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Apicella M. A., Dudas K. C., Campagnari A., Rice P., Mylotte J. M., Murphy T. F. Antigenic heterogeneity of lipid A of Haemophilus influenzae. Infect Immun. 1985 Oct;50(1):9–14. doi: 10.1128/iai.50.1.9-14.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barenkamp S. J., Munson R. S., Jr, Granoff D. M. Subtyping isolates of Haemophilus influenzae type b by outer-membrane protein profiles. J Infect Dis. 1981 May;143(5):668–676. doi: 10.1093/infdis/143.5.668. [DOI] [PubMed] [Google Scholar]
  3. Berk S. L., Holtsclaw S. A., Wiener S. L., Smith J. K. Nontypeable Haemophilus influenzae in the elderly. Arch Intern Med. 1982 Mar;142(3):537–539. [PubMed] [Google Scholar]
  4. Evans F. O., Jr, Sydnor J. B., Moore W. E., Moore G. R., Manwaring J. L., Brill A. H., Jackson R. T., Hanna S., Skaar J. S., Holdeman L. V. Sinusitis of the maxillary antrum. N Engl J Med. 1975 Oct 9;293(15):735–739. doi: 10.1056/NEJM197510092931502. [DOI] [PubMed] [Google Scholar]
  5. Gnehm H. E., Pelton S. I., Gulati S., Rice P. A. Characterization of antigens from nontypable Haemophilus influenzae recognized by human bactericidal antibodies. Role of Haemophilus outer membrane proteins. J Clin Invest. 1985 May;75(5):1645–1658. doi: 10.1172/JCI111872. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Klein J. O. Microbiology and antimicrobial treatment of otitis media. Ann Otol Rhinol Laryngol Suppl. 1981 May-Jun;90(3 Pt 3):30–36. doi: 10.1177/00034894810903s209. [DOI] [PubMed] [Google Scholar]
  7. Loeb M. R., Smith D. H. Outer membrane protein composition in disease isolates of Haemophilus influenzae: pathogenic and epidemiological implications. Infect Immun. 1980 Dec;30(3):709–717. doi: 10.1128/iai.30.3.709-717.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Munson R. S., Jr, Granoff D. M. Purification and partial characterization of outer membrane proteins P5 and P6 from Haemophilus influenzae type b. Infect Immun. 1985 Sep;49(3):544–549. doi: 10.1128/iai.49.3.544-549.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Munson R. S., Jr, Shenep J. L., Barenkamp S. J., Granoff D. M. Purification and comparison of outer membrane protein P2 from Haemophilus influenzae type b isolates. J Clin Invest. 1983 Aug;72(2):677–684. doi: 10.1172/JCI111017. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Murphy T. F., Apicella M. A. Antigenic heterogeneity of outer membrane proteins of nontypable Haemophilus influenzae is a basis for a serotyping system. Infect Immun. 1985 Oct;50(1):15–21. doi: 10.1128/iai.50.1.15-21.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Murphy T. F., Bartos L. C., Rice P. A., Nelson M. B., Dudas K. C., Apicella M. A. Identification of a 16,600-dalton outer membrane protein on nontypeable Haemophilus influenzae as a target for human serum bactericidal antibody. J Clin Invest. 1986 Oct;78(4):1020–1027. doi: 10.1172/JCI112656. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Murphy T. F., Dudas K. C., Mylotte J. M., Apicella M. A. A subtyping system for nontypable Haemophilus influenzae based on outer-membrane proteins. J Infect Dis. 1983 May;147(5):838–846. doi: 10.1093/infdis/147.5.838. [DOI] [PubMed] [Google Scholar]
  13. Murphy T. F., Nelson M. B., Dudas K. C., Mylotte J. M., Apicella M. A. Identification of a specific epitope of Haemophilus influenzae on a 16,600-dalton outer membrane protein. J Infect Dis. 1985 Dec;152(6):1300–1307. doi: 10.1093/infdis/152.6.1300. [DOI] [PubMed] [Google Scholar]
  14. Musher D. M., Kubitschek K. R., Crennan J., Baughn R. E. Pneumonia and acute febrile tracheobronchitis due to haemophilus influenzae. Ann Intern Med. 1983 Oct;99(4):444–450. doi: 10.7326/0003-4819-99-4-444. [DOI] [PubMed] [Google Scholar]
  15. Myhre E. B. Typing of Haemophilus influenzae by counterimmunoelectrophoresis. Acta Pathol Microbiol Scand B Microbiol Immunol. 1974 Apr;82(2):164–166. doi: 10.1111/j.1699-0463.1974.tb02308.x. [DOI] [PubMed] [Google Scholar]
  16. Spagnuolo P. J., Ellner J. J., Lerner P. I., McHenry M. C., Flatauer F., Rosenberg P., Rosenthal M. S. Haemophilus influenzae meningitis: the spectrum of disease in adults. Medicine (Baltimore) 1982 Mar;61(2):74–85. [PubMed] [Google Scholar]
  17. Wallace R. J., Jr, Baker C. J., Quinones F. J., Hollis D. G., Weaver R. E., Wiss K. Nontypable Haemophilus influenzae (biotype 4) as a neonatal, maternal, and genital pathogen. Rev Infect Dis. 1983 Jan-Feb;5(1):123–136. doi: 10.1093/clinids/5.1.123. [DOI] [PubMed] [Google Scholar]
  18. Wallace R. J., Jr, Musher D. M., Septimus E. J., McGowan J. E., Jr, Quinones F. J., Wiss K., Vance P. H., Trier P. A. Haemophilus influenzae infections in adults: characterization of strains by serotypes, biotypes, and beta-lactamase production. J Infect Dis. 1981 Aug;144(2):101–106. doi: 10.1093/infdis/144.2.101. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES