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. 1986 Dec;54(3):924–927. doi: 10.1128/iai.54.3.924-927.1986

Stability of expression of Neisseria gonorrhoeae lipooligosaccharides.

H Schneider, C A Hammack, B A Shuman, J M Griffiss
PMCID: PMC260264  PMID: 2430891

Abstract

We compared multiple lipooligosaccharide (LOS) extracts from individual strains of Neisseria gonorrhoeae. Each of the extracts was prepared from single mass cultures grown on solid media under similar conditions but separated by time. We found only subtle variations in the number, electrophoretic mobility, and concentration of components of the LOSs from individual strains. We found no variation in the expression of a 3.6-kilodalton LOS component that carries the L8 LOS epitope. A significant variation in the 3-deoxy-D-manno-octulosonic acid content was found among different extracts from the same strain, but this variation appeared to be unrelated to the other LOS characteristics studied.

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Selected References

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  1. Apicella M. A., Westerink M. A., Morse S. A., Schneider H., Rice P. A., Griffiss J. M. Bactericidal antibody response of normal human serum to the lipooligosaccharide of Neisseria gonorrhoeae. J Infect Dis. 1986 Mar;153(3):520–526. doi: 10.1093/infdis/153.3.520. [DOI] [PubMed] [Google Scholar]
  2. Bertram M. A., Griffiss J. M., Broud D. D. Response to antigenic determinants of Neisseria meningitidis lipopolysaccharide investigated with a new radioactive antigen-binding assay. J Immunol. 1976 Mar;116(3):842–846. [PubMed] [Google Scholar]
  3. Burnette W. N. "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal Biochem. 1981 Apr;112(2):195–203. doi: 10.1016/0003-2697(81)90281-5. [DOI] [PubMed] [Google Scholar]
  4. Connelly M. C., Allen P. Z. Chemical and immunochemical studies on lipopolysaccharides from pyocin 103-sensitive and -resistant Neisseria gonorrhoeae. Carbohydr Res. 1983 Aug 16;120:171–186. doi: 10.1016/0008-6215(83)88015-x. [DOI] [PubMed] [Google Scholar]
  5. Lammel C. J., Sweet R. L., Rice P. A., Knapp J. S., Schoolnik G. K., Heilbron D. C., Brooks G. F. Antibody-antigen specificity in the immune response to infection with Neisseria gonorrhoeae. J Infect Dis. 1985 Nov;152(5):990–1001. doi: 10.1093/infdis/152.5.990. [DOI] [PubMed] [Google Scholar]
  6. Mandrell R., Schneider H., Apicella M., Zollinger W., Rice P. A., Griffiss J. M. Antigenic and physical diversity of Neisseria gonorrhoeae lipooligosaccharides. Infect Immun. 1986 Oct;54(1):63–69. doi: 10.1128/iai.54.1.63-69.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. McDonald I. J., Adams G. A. Influence of cultural conditions on the lipopolysaccharide composition of Neisseria sicca. J Gen Microbiol. 1971 Feb;65(2):201–207. doi: 10.1099/00221287-65-2-201. [DOI] [PubMed] [Google Scholar]
  8. Morse S. A., Apicella M. A. Isolation of a lipopolysaccharide mutant of Neisseria gonorrhoeae: an analysis of the antigenic and biologic difference. J Infect Dis. 1982 Feb;145(2):206–216. doi: 10.1093/infdis/145.2.206. [DOI] [PubMed] [Google Scholar]
  9. Morse S. A., Mintz C. S., Sarafian S. K., Bartenstein L., Bertram M., Apicella M. A. Effect of dilution rate on lipopolysaccharide and serum resistance of Neisseria gonorrhoeae grown in continuous culture. Infect Immun. 1983 Jul;41(1):74–82. doi: 10.1128/iai.41.1.74-82.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. OSBORN M. J. STUDIES ON THE GRAM-NEGATIVE CELL WALL. I. EVIDENCE FOR THE ROLE OF 2-KETO- 3-DEOXYOCTONATE IN THE LIPOPOLYSACCHARIDE OF SALMONELLA TYPHIMURIUM. Proc Natl Acad Sci U S A. 1963 Sep;50:499–506. doi: 10.1073/pnas.50.3.499. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Poolman J. T., Hopman C. T., Zanen H. C. Colony variants of Neisseria meningitidis strain 2996 (B:2b:P1.2): influence of class-5 outer membrane proteins and lipopolysaccharides. J Med Microbiol. 1985 Apr;19(2):203–209. doi: 10.1099/00222615-19-2-203. [DOI] [PubMed] [Google Scholar]
  12. Rice P. A., McCormack W. M., Kasper D. L. Natural serum bactericidal activity against Neisseria gonorrhoeae isolates from disseminated, locally invasive, and uncomplicated disease. J Immunol. 1980 May;124(5):2105–2109. [PubMed] [Google Scholar]
  13. Schneider H., Griffiss J. M., Mandrell R. E., Jarvis G. A. Elaboration of a 3.6-kilodalton lipooligosaccharide, antibody against which is absent from human sera, is associated with serum resistance of Neisseria gonorrhoeae. Infect Immun. 1985 Dec;50(3):672–677. doi: 10.1128/iai.50.3.672-677.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Schneider H., Griffiss J. M., Williams G. D., Pier G. B. Immunological basis of serum resistance of Neisseria gonorrhoeae. J Gen Microbiol. 1982 Jan;128(1):13–22. doi: 10.1099/00221287-128-1-13. [DOI] [PubMed] [Google Scholar]
  15. Schneider H., Hale T. L., Zollinger W. D., Seid R. C., Jr, Hammack C. A., Griffiss J. M. Heterogeneity of molecular size and antigenic expression within lipooligosaccharides of individual strains of Neisseria gonorrhoeae and Neisseria meningitidis. Infect Immun. 1984 Sep;45(3):544–549. doi: 10.1128/iai.45.3.544-549.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Stead A., Main J. S., Ward M. E., Watt P. J. Studies on lipopolysaccharides isolated from strains of Neisseria gonorrhoeae. J Gen Microbiol. 1975 May;88(1):123–131. doi: 10.1099/00221287-88-1-123. [DOI] [PubMed] [Google Scholar]
  17. Tsai C. M., Frasch C. E. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal Biochem. 1982 Jan 1;119(1):115–119. doi: 10.1016/0003-2697(82)90673-x. [DOI] [PubMed] [Google Scholar]
  18. Zollinger W. D., Mandrell R. E. Importance of complement source in bactericidal activity of human antibody and murine monoclonal antibody to meningococcal group B polysaccharide. Infect Immun. 1983 Apr;40(1):257–264. doi: 10.1128/iai.40.1.257-264.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]

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