Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1987 Mar;55(3):810–815. doi: 10.1128/iai.55.3.810-815.1987

Recognition of carbohydrate and protein epitopes by monoclonal antibodies to a cell wall antigen from Streptococcus mutans.

C G Mitchell, R Smith, T Lehner
PMCID: PMC260414  PMID: 2434429

Abstract

The nature of the determinants recognized by a panel of monoclonal antibodies (MAbs) raised against a cell wall antigen of Streptococcus mutans (SA I/II) was investigated. Mild periodate oxidation of SA I/II showed that MAbs Guy 1, 2, 3, and 5 recognized carbohydrate epitopes on the antigen. Glycosidases were used to identify the nature of the sugars involved in their binding. Treatment with beta-glucosidase inhibited the binding of Guy 1, 2, 3, and 5 by 90%. No competition was found for any of the MAbs between SA I/II and a series of carbohydrates, including the serotype c polysaccharide from S. mutans. The results show that MAbs Guy 1, 2, 3, and 5 recognize carbohydrate epitopes on SA I/II which are distinct from the serotype polysaccharide. The other MAbs recognized protein epitopes on SA I/II.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BOBBITT J. M. Periodate oxidation of carbohydrates. Adv Carbohydr Chem. 1956;48(11):1–41. doi: 10.1016/s0096-5332(08)60115-0. [DOI] [PubMed] [Google Scholar]
  2. CLAMP J. R., HOUGH L. THE PERIODATE OXIDATION OF AMINO ACIDS WITH REFERENCE TO STUDIES ON GLYCOPROTEINS. Biochem J. 1965 Jan;94:17–24. doi: 10.1042/bj0940017. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Carlsson J., Newbrun E., Krasse B. Purification and properties of dextransucrase from Streptococcus sanguis. Arch Oral Biol. 1969 May;14(5):469–478. doi: 10.1016/0003-9969(69)90140-x. [DOI] [PubMed] [Google Scholar]
  4. Giasuddin A. S., Lehner T., Evans R. W. Identification, purification and characterization of a streptococcal protein antigen with a molecular weight of 3800. Immunology. 1983 Dec;50(4):651–658. [PMC free article] [PubMed] [Google Scholar]
  5. Guggenheim B., Newbrun E. Extracellular glucosyltransferase activity of an HS strain of Streptococcus mutans. Helv Odontol Acta. 1969 Oct;13(2):84–97. [PubMed] [Google Scholar]
  6. HUNTER W. M., GREENWOOD F. C. Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature. 1962 May 5;194:495–496. doi: 10.1038/194495a0. [DOI] [PubMed] [Google Scholar]
  7. Hamada S., Slade H. D. Biology, immunology, and cariogenicity of Streptococcus mutans. Microbiol Rev. 1980 Jun;44(2):331–384. doi: 10.1128/mr.44.2.331-384.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lehner T., Caldwell J., Smith R. Local passive immunization by monoclonal antibodies against streptococcal antigen I/II in the prevention of dental caries. Infect Immun. 1985 Dec;50(3):796–799. doi: 10.1128/iai.50.3.796-799.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lehner T., Russell M. W., Caldwell J., Smith R. Immunization with purified protein antigens from Streptococcus mutans against dental caries in rhesus monkeys. Infect Immun. 1981 Nov;34(2):407–415. doi: 10.1128/iai.34.2.407-415.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Linzer R., Gill K., Slade H. D. Chemical composition of Streptococcus mutans type c antigen: comparison to type a, b, and d antigens. J Dent Res. 1976 Jan;55:A109–A115. doi: 10.1177/002203457605500103011. [DOI] [PubMed] [Google Scholar]
  11. McCabe M. M., Hamelik R. M., Smith E. E. Purification of dextran-binding protein from cariogenic Streptococcus mutans. Biochem Biophys Res Commun. 1977 Sep 9;78(1):273–278. doi: 10.1016/0006-291x(77)91250-5. [DOI] [PubMed] [Google Scholar]
  12. McCabe M. M., Smith E. E. Relationship between cell-bound dextransucrase and the agglutination of Streptococcus mutans. Infect Immun. 1975 Sep;12(3):512–520. doi: 10.1128/iai.12.3.512-520.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Russell M. W., Bergmeier L. A., Zanders E. D., Lehner T. Protein antigens of Streptococcus mutans: purification and properties of a double antigen and its protease-resistant component. Infect Immun. 1980 May;28(2):486–493. doi: 10.1128/iai.28.2.486-493.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Russell M. W., Lehner T. Characterisation of antigens extracted from cells and culture fluids of Streptococcus mutans serotype c. Arch Oral Biol. 1978;23(1):7–15. doi: 10.1016/0003-9969(78)90047-x. [DOI] [PubMed] [Google Scholar]
  15. Russell M. W. Purification and properties of a protein surface antigen of Streptococcus mutants. Microbios. 1979;25(99):7–18. [PubMed] [Google Scholar]
  16. Russell M. W., Zanders E. D., Bergmeier L. A., Lehner T. Affinity purification and characterization of protease-susceptible antigen I of Streptococcus mutans. Infect Immun. 1980 Sep;29(3):999–1006. doi: 10.1128/iai.29.3.999-1006.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Russell R. R. Wall-associated protein antigens of Streptococcus mutans. J Gen Microbiol. 1979 Sep;114(1):109–115. doi: 10.1099/00221287-114-1-109. [DOI] [PubMed] [Google Scholar]
  18. Schöller M., Klein J. P., Frank R. M. Common antigens of streptococcal and non-streptococcal oral bacteria: immunochemical studies of extracellular and cell-wall-associated antigens from Streptococcus sanguis, Streptococcus mutans, Lactobacillus salivarius, and Actinomyces viscosus. Infect Immun. 1981 Jan;31(1):52–60. doi: 10.1128/iai.31.1.52-60.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Slade H. D., Slamp W. C. Peptidoglycan composition and taxonomy of group D, E, and H streptococci, and Streptococcus mutans. J Bacteriol. 1972 Feb;109(2):691–695. doi: 10.1128/jb.109.2.691-695.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Smith R., Lehner T. A radioimmunoassay for serum and gingival crevicular fluid antibodies to a purified protein of Streptococcus mutans. Clin Exp Immunol. 1981 Feb;43(2):417–424. [PMC free article] [PubMed] [Google Scholar]
  21. Smith R., Lehner T., Beverley P. C. Characterization of monoclonal antibodies to Streptococcus mutans antigenic determinants I/II, I, II, and III and their serotype specificities. Infect Immun. 1984 Oct;46(1):168–175. doi: 10.1128/iai.46.1.168-175.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Woodward M. P., Young W. W., Jr, Bloodgood R. A. Detection of monoclonal antibodies specific for carbohydrate epitopes using periodate oxidation. J Immunol Methods. 1985 Apr 8;78(1):143–153. doi: 10.1016/0022-1759(85)90337-0. [DOI] [PubMed] [Google Scholar]
  23. Zanders E. D., Lehner T. Separation and characterization of a protein antigen from cells of Streptococcus mutans. J Gen Microbiol. 1981 Feb;122(2):217–225. doi: 10.1099/00221287-122-2-217. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES