Abstract
Lymphocyte transformation (LT) responses to coccidioidin (CDN) and spherulin were suppressed in 11 (73%) of 15 patients with active coccidioidomycosis when their mononuclear cells were assayed in autologous serum as compared to serum from healthy, CDN skin test-positive subjects. Suppressed LT responses were specific for Coccidioides immitis antigens in 7 (64%) of the 11 patients. Immunoaffinity chromatography of patient sera with Staphylococcus protein A adsorbed the suppressor component(s) and thereby established that suppression was attributed to immunoglobulin G, either alone or complexed with antigen. The possibility that suppression was mediated by immune complexes was examined by adding complexes formed in vivo or in vitro to mononuclear cell cultures of healthy CDN-reactive persons before LT assays. Although complexes prepared in this manner were reactive in an enzyme-linked immunosorbent assay designed to detect Coccidioides antigen-specific immune complexes, no suppression of LT responses was observed. We conclude that serum-mediated suppression of LT responses in coccidioidomycosis is attributed to monomeric and not immune-complexed immunoglobulin G antibody.
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- Beaman L. V., Pappagianis D., Benjamini E. Mechanisms of resistance to infection with Coccidioides immitis in mice. Infect Immun. 1979 Mar;23(3):681–685. doi: 10.1128/iai.23.3.681-685.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Beaman L., Pappagianis D., Benjamini E. Significance of T cells in resistance to experimental murine coccidioidomycosis. Infect Immun. 1977 Sep;17(3):580–585. doi: 10.1128/iai.17.3.580-585.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Böyum A. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest Suppl. 1968;97:77–89. [PubMed] [Google Scholar]
- Catanzaro A., Spitler L. E., Moser K. M. Cellular immune response in coccidioidomycosis. Cell Immunol. 1975 Feb;15(2):360–371. doi: 10.1016/0008-8749(75)90014-3. [DOI] [PubMed] [Google Scholar]
- Cox R. A., Brummer E., Lecara G. In vitro lymphocyte responses of coccidioidin skin test-positive and -negative persons to coccidioidin, spherulin, and a coccidioides cell wall antigen. Infect Immun. 1977 Mar;15(3):751–755. doi: 10.1128/iai.15.3.751-755.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cox R. A., Pope R. M., Stevens D. A. Immune complexes in coccidioidomycosis. Correlation with disease involvement. Am Rev Respir Dis. 1982 Sep;126(3):439–443. doi: 10.1164/arrd.1982.126.3.439. [DOI] [PubMed] [Google Scholar]
- Cox R. A., Vivas J. R., Gross A., Lecara G., Miller E., Brummer E. In vivo and in vitro cell-mediated responses in coccidioidomycosis. I. Immumologic responses of persons with primary, asymptomatic infections. Am Rev Respir Dis. 1976 Nov;114(5):937–943. doi: 10.1164/arrd.1976.114.5.937. [DOI] [PubMed] [Google Scholar]
- Cox R. A., Vivas J. R. Spectrum of in vivo and in vitro cell-mediated immune responses in coccidioidomycosis. Cell Immunol. 1977 Jun 1;31(1):130–141. doi: 10.1016/0008-8749(77)90012-0. [DOI] [PubMed] [Google Scholar]
- Dorval G., Yang W. H., Goodfriend L., Roy R., Espinoza L. R., Hébert J. Circulating immune complexes may be associated with increased suppressor T-cell activity in atopic allergy. Clin Immunol Immunopathol. 1980 Jun;16(2):245–253. doi: 10.1016/0090-1229(80)90209-3. [DOI] [PubMed] [Google Scholar]
- Eichmann K. Idiotype suppression. I. Influence of the dose and of the effector functions of anti-idiotypic antibody on the production of an idiotype. Eur J Immunol. 1974 Apr;4(4):296–302. doi: 10.1002/eji.1830040413. [DOI] [PubMed] [Google Scholar]
- Eichmann K. Idiotype suppression. II. Amplification of a suppressor T cell with anti-idiotypic activity. Eur J Immunol. 1975 Aug;5(8):511–517. doi: 10.1002/eji.1830050802. [DOI] [PubMed] [Google Scholar]
- Geha R. S. Idiotypic determinants on human T cells and modulation of human T cell responses by anti-idiotypic antibodies. J Immunol. 1984 Oct;133(4):1846–1851. [PubMed] [Google Scholar]
- Gershon R. K., Mokyr M. B., Mitchell M. S. Activation of suppressor T cells by tumour cells and specific antibody. Nature. 1974 Aug 16;250(467):594–596. doi: 10.1038/250594a0. [DOI] [PubMed] [Google Scholar]
- Harvey R. P., Stevens D. A. In vitro assays of cellular immunity in progressive coccidioidomycosis: evaluation of suppression with parasitic-phase antigen. Am Rev Respir Dis. 1981 Jun;123(6):665–669. doi: 10.1164/arrd.1981.123.6.665. [DOI] [PubMed] [Google Scholar]
- Ibrahim A. B., Pappagianis D. Experimental induction of anergy to coccidioidin by antigens of Coccidioides immitis. Infect Immun. 1973 May;7(5):786–794. doi: 10.1128/iai.7.5.786-794.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kamal K. A., Higashi G. I. Suppression of mitogen-induced lymphocyte transformation by plasma from patients with hepatosplenic Schistosomiasis mansoni: role of immune complexes. Parasite Immunol. 1982 Jul;4(4):283–298. doi: 10.1111/j.1365-3024.1982.tb00439.x. [DOI] [PubMed] [Google Scholar]
- Lipsky P. E. Staphylococcal protein A, a T cell-regulated polyclonal activator of human B cells. J Immunol. 1980 Jul;125(1):155–162. [PubMed] [Google Scholar]
- McDougal J. S., Redecha P. B., Inman R. D., Christian C. L. Binding of immunoglobulin G aggregates and immune complexes in human sera to Staphylococci containing protein A. J Clin Invest. 1979 Apr;63(4):627–636. doi: 10.1172/JCI109345. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Opelz G., Scheer M. I. Cutaneous sensitivity and in vitro responsiveness of lymphocytes in patients with disseminated coccidioidomycosis. J Infect Dis. 1975 Sep;132(3):250–255. doi: 10.1093/infdis/132.3.250. [DOI] [PubMed] [Google Scholar]
- Rao V. S., Bennett J. A., Shen F. W., Gershon R. K., Mitchell M. S. Antigen-antibody complexes generate Lyt 1 inducers of suppressor cells. J Immunol. 1980 Jul;125(1):63–67. [PubMed] [Google Scholar]
- Rea T. H., Einstein H., Levan N. E. Dinitrochlorobenzene responsivity in disseminated coccidioidomycosis: an inverse correlation with complement-fixing antibody titers. J Invest Dermatol. 1976 Jan;66(1):34–37. doi: 10.1111/1523-1747.ep12478071. [DOI] [PubMed] [Google Scholar]
- Rowley D. A., Fitch F. W., Stuart F. P., Köhler H., Cosenza H. Specific suppression of immune responses. Science. 1973 Sep 21;181(4105):1133–1141. doi: 10.1126/science.181.4105.1133. [DOI] [PubMed] [Google Scholar]
- SMITH C. E., SAITO M. T., BEARD R. R., KEPP R. M., CLARK R. W., EDDIE B. U. Serological tests in the diagnosis and prognosis of coccidioidomycosis. Am J Hyg. 1950 Jul;52(1):1–21. doi: 10.1093/oxfordjournals.aje.a119404. [DOI] [PubMed] [Google Scholar]
- SMITH C. E., SAITO M. T., SIMONS S. A. Pattern of 39,500 serologic tests in coccidioidomycosis. J Am Med Assoc. 1956 Feb 18;160(7):546–552. doi: 10.1001/jama.1956.02960420026008. [DOI] [PubMed] [Google Scholar]
- Sinclair N. R. Modulation of immunity by antibody, antigen-antibody complexes and antigen. Pharmacol Ther. 1979;4(2):355–432. doi: 10.1016/0163-7258(79)90142-6. [DOI] [PubMed] [Google Scholar]
- Sy M. S., Bach B. A., Dohi Y., Nisonoff A., Benacerraf B., Greene M. I. Antigen- and receptor-driven regulatory mechanisms. I. Induction of suppressor T cells with anti-idiotypic antibodies. J Exp Med. 1979 Nov 1;150(5):1216–1228. doi: 10.1084/jem.150.5.1216. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Taylor R. B., Tite J. P., Manzo C. Immunoregulatory effects of a covalent antigen-antibody complex. Nature. 1979 Oct 11;281(5731):488–490. doi: 10.1038/281488a0. [DOI] [PubMed] [Google Scholar]
- Virgin H. W., 4th, Wittenberg G. F., Bancroft G. J., Unanue E. R. Suppression of immune response to Listeria monocytogenes: mechanism(s) of immune complex suppression. Infect Immun. 1985 Nov;50(2):343–353. doi: 10.1128/iai.50.2.343-353.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yoshinoya S., Cox R. A., Pope R. M. Circulating immune complexes in coccidioidomycosis. Detection and characterization. J Clin Invest. 1980 Oct;66(4):655–663. doi: 10.1172/JCI109901. [DOI] [PMC free article] [PubMed] [Google Scholar]