Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1987 Jun;55(6):1484–1489. doi: 10.1128/iai.55.6.1484-1489.1987

Effect of crevicular fluid and lysosomal enzymes on the adherence of streptococci and bacteroides to hydroxyapatite.

G Cimasoni, M Song, B C McBride
PMCID: PMC260540  PMID: 3032801

Abstract

Samples of hydroxyapatite (HA) beads smaller than 1 mg were coated with 10 microliter of either saliva, serum, or human crevicular fluid before being added to a suspension of Streptococcus sanguis or Bacteroides gingivalis. In some assays, preparations of a granular fraction, elastase, or cathepsin G from human leukocytes were used to coat HA or to treat saliva-coated HA (SHA) before mixing with bacteria. The number of cells adhering to the beads was then counted under a scanning electron microscope by a standardized procedure. More cells were found to adhere to SHA in this assay than in the conventional large-scale assay. Human crevicular fluid, even when diluted up to three times, completely inhibited the adherence of S. sanguis to HA. A 100% inhibition of S. sanguis adherence was also observed when HA was coated with the granular fraction of leukocytes, and a 65% inhibition observed when SHA was treated with the enzyme preparation. When used to coat HA, elastase and cathepsin G reduced the adherence of S. sanguis by 30 and 50%, respectively. The binding of S. sanguis to elastase- or cathepsin G-treated SHA was also reduced. B. gingivalis 33277 was found to adhere in high numbers to SHA. Coating HA with crevicular fluid or with the lysosomal enzyme preparation had a limited negative effect. We postulate that crevicular fluid prevents the adherence of S. sanguis by virtue of either its enzyme content or its albumin content or both.

Full text

PDF
1484

Images in this article

1486Image
on p.1486
1487Image
on p.1487

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Appelbaum B., Golub E., Holt S. C., Rosan B. In vitro studies of dental plaque formation: adsorption of oral streptococci to hydroxyaptite. Infect Immun. 1979 Aug;25(2):717–728. doi: 10.1128/iai.25.2.717-728.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baugh R. J., Travis J. Human leukocyte granule elastase: rapid isolation and characterization. Biochemistry. 1976 Feb 24;15(4):836–841. doi: 10.1021/bi00649a017. [DOI] [PubMed] [Google Scholar]
  3. Bickel M., Cimasoni G., Andersen E. Flow and albumin content of early (pre-inflammatory) gingival crevicular fluid from human subjects. Arch Oral Biol. 1985;30(8):599–602. doi: 10.1016/0003-9969(85)90079-2. [DOI] [PubMed] [Google Scholar]
  4. Boyd J., McBride B. C. Fractionation of hemagglutinating and bacterial binding adhesins of Bacteroides gingivalis. Infect Immun. 1984 Aug;45(2):403–409. doi: 10.1128/iai.45.2.403-409.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gibbons R. J., Etherden I. Albumin as a blocking agent in studies of streptococcal adsorption to experimental salivary pellicles. Infect Immun. 1985 Nov;50(2):592–594. doi: 10.1128/iai.50.2.592-594.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gibbons R. J., Houte J. V. Bacterial adherence in oral microbial ecology. Annu Rev Microbiol. 1975;29:19–44. doi: 10.1146/annurev.mi.29.100175.000315. [DOI] [PubMed] [Google Scholar]
  7. Liljemark W. F., Schauer S. V., Bloomquist C. G. Compounds which affect the adherence of Streptococcus sanguis and Streptococcus mutans to hydroxyapatite. J Dent Res. 1978 Feb;57(2):373–379. doi: 10.1177/00220345780570023901. [DOI] [PubMed] [Google Scholar]
  8. McBride B. C., Gisslow M. T. Role of sialic acid in saliva-induced aggregation of Streptococcus sanguis. Infect Immun. 1977 Oct;18(1):35–40. doi: 10.1128/iai.18.1.35-40.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. McDonald J. A., Baum B. J., Rosenberg D. M., Kelman J. A., Brin S. C., Crystal R. G. Destruction of a major extracellular adhesive glycoprotein (fibronectin) of human fibroblasts by neutral proteases from polymorphonuclear leukocyte granules. Lab Invest. 1979 Mar;40(3):350–357. [PubMed] [Google Scholar]
  10. Morris E. J., McBride B. C. Adherence of Streptococcus sanguis to saliva-coated hydroxyapatite: evidence for two binding sites. Infect Immun. 1984 Feb;43(2):656–663. doi: 10.1128/iai.43.2.656-663.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Morris E. J., McBride B. C. Aggregation of Streptococcus sanguis by a neuraminidase-sensitive component of serum and crevicular fluid. Infect Immun. 1983 Dec;42(3):1073–1080. doi: 10.1128/iai.42.3.1073-1080.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Okuda K., Takazoe I. Haemagglutinating activity of Bacteroides melaninogenicus. Arch Oral Biol. 1974 May;19(5):415–416. doi: 10.1016/0003-9969(74)90184-8. [DOI] [PubMed] [Google Scholar]
  13. SKOOG W. A., BECK W. S. Studies on the fibrinogen, dextran and phytohemagglutinin methods of isolating leukocytes. Blood. 1956 May;11(5):436–454. [PubMed] [Google Scholar]
  14. Sela M. N., Natan G., Lahav M., Ginsburg I., Dishon T. Bacteriolytic activity of human gingival exudate. Inflammation. 1980 Jun;4(2):195–203. doi: 10.1007/BF00914165. [DOI] [PubMed] [Google Scholar]
  15. Slots J., Gibbons R. J. Attachment of Bacteroides melaninogenicus subsp. asaccharolyticus to oral surfaces and its possible role in colonization of the mouth and of periodontal pockets. Infect Immun. 1978 Jan;19(1):254–264. doi: 10.1128/iai.19.1.254-264.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sueda T., Bang J., Cimasoni G. Collection of gingival fluid for quantitative analysis. J Dent Res. 1969 Jan-Feb;48(1):159–159. doi: 10.1177/00220345690480011501. [DOI] [PubMed] [Google Scholar]
  17. Virca G. D., Metz G., Schnebli H. P. Similarities between human and rat leukocyte elastase and cathepsin G. Eur J Biochem. 1984 Oct 1;144(1):1–9. doi: 10.1111/j.1432-1033.1984.tb08423.x. [DOI] [PubMed] [Google Scholar]
  18. Wikström M., Linde A. Ability of oral bacteria to degrade fibronectin. Infect Immun. 1986 Feb;51(2):707–711. doi: 10.1128/iai.51.2.707-711.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Woo D. D., Holt S. C., Leadbetter E. R. Ultrastructure of Bacteroides species: Bacteroides asaccharolyticus, Bacteroides fragilis, Bacteroides melaninogenicus subspecies melaninogenicus, and B. melaninogenicus subspecies intermedius. J Infect Dis. 1979 May;139(5):534–546. doi: 10.1093/infdis/139.5.534. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES