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. 1987 Jun;55(6):1529–1532. doi: 10.1128/iai.55.6.1529-1532.1987

Transcription of cholera toxin operon in wild-type and mutant strains of Vibrio cholerae.

L Mishra, R K Holmes
PMCID: PMC260548  PMID: 3570476

Abstract

mRNA for cholera toxin (CT) was measured in wild-type, hypertoxinogenic, and hypotoxinogenic strains of the classical biotype. The amount of CT-specific mRNA was directly related to the amount of CT that the strains could produce. Estimates of the size of the mRNA for CT in individual experiments varied between approximately 900 and 1,100 nucleotides.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alwine J. C., Kemp D. J., Parker B. A., Reiser J., Renart J., Stark G. R., Wahl G. M. Detection of specific RNAs or specific fragments of DNA by fractionation in gels and transfer to diazobenzyloxymethyl paper. Methods Enzymol. 1979;68:220–242. doi: 10.1016/0076-6879(79)68017-5. [DOI] [PubMed] [Google Scholar]
  2. Baine W. B., Vasil M. L., Holmes R. K. Genetic mapping of mutations in independently isolated nontoxinogenic mutants of Vibrio cholerae. Infect Immun. 1978 Jul;21(1):194–200. doi: 10.1128/iai.21.1.194-200.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bramucci M. G., Twiddy E. M., Baine W. B., Holmes R. K. Isolation and characterization of hypertoxinogenic (htx) mutants of Escherichia coli KL320(pCG86). Infect Immun. 1981 Jun;32(3):1034–1044. doi: 10.1128/iai.32.3.1034-1044.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chirgwin J. M., Przybyla A. E., MacDonald R. J., Rutter W. J. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979 Nov 27;18(24):5294–5299. doi: 10.1021/bi00591a005. [DOI] [PubMed] [Google Scholar]
  6. Holmes R. K., Baine W. B., Vasil M. L. Quantitative measurements of cholera enterotoxin in cultures of toxinogenic wild-type and nontoxinogenic mutant strains of Vibrio cholerae by using a sensitive and specific reversed passive hemagglutination assay for cholera enerotoxin. Infect Immun. 1978 Jan;19(1):101–106. doi: 10.1128/iai.19.1.101-106.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Holmes R. K., Twiddy E. M. Characterization of monoclonal antibodies that react with unique and cross-reacting determinants of cholera enterotoxin and its subunits. Infect Immun. 1983 Dec;42(3):914–923. doi: 10.1128/iai.42.3.914-923.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Honda T., Finkelstein R. A. Selection and characteristics of a Vibrio cholerae mutant lacking the A (ADP-ribosylating) portion of the cholera enterotoxin. Proc Natl Acad Sci U S A. 1979 Apr;76(4):2052–2056. doi: 10.1073/pnas.76.4.2052. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Johnson S. R., Romig W. R. Transposon-facilitated recombination in Vibrio cholerae. Mol Gen Genet. 1979 Feb 16;170(1):93–101. doi: 10.1007/BF00268584. [DOI] [PubMed] [Google Scholar]
  10. Kafatos F. C., Jones C. W., Efstratiadis A. Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure. Nucleic Acids Res. 1979 Nov 24;7(6):1541–1552. doi: 10.1093/nar/7.6.1541. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kaper J. B., Lockman H., Baldini M. M., Levine M. M. Recombinant nontoxinogenic Vibrio cholerae strains as attenuated cholera vaccine candidates. Nature. 1984 Apr 12;308(5960):655–658. doi: 10.1038/308655a0. [DOI] [PubMed] [Google Scholar]
  12. Lockman H., Kaper J. B. Nucleotide sequence analysis of the A2 and B subunits of Vibrio cholerae enterotoxin. J Biol Chem. 1983 Nov 25;258(22):13722–13726. [PubMed] [Google Scholar]
  13. Mekalanos J. J. Duplication and amplification of toxin genes in Vibrio cholerae. Cell. 1983 Nov;35(1):253–263. doi: 10.1016/0092-8674(83)90228-3. [DOI] [PubMed] [Google Scholar]
  14. Mekalanos J. J., Murphy J. R. Regulation of cholera toxin production in Vibrio cholerae: genetic analysis of phenotypic instability in hypertoxinogenic mutants. J Bacteriol. 1980 Feb;141(2):570–576. doi: 10.1128/jb.141.2.570-576.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mekalanos J. J., Sublett R. D., Romig W. R. Genetic mapping of toxin regulatory mutations in Vibrio cholerae. J Bacteriol. 1979 Sep;139(3):859–865. doi: 10.1128/jb.139.3.859-865.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Mekalanos J. J., Swartz D. J., Pearson G. D., Harford N., Groyne F., de Wilde M. Cholera toxin genes: nucleotide sequence, deletion analysis and vaccine development. Nature. 1983 Dec 8;306(5943):551–557. doi: 10.1038/306551a0. [DOI] [PubMed] [Google Scholar]
  17. Miller V. L., Mekalanos J. J. Genetic analysis of the cholera toxin-positive regulatory gene toxR. J Bacteriol. 1985 Aug;163(2):580–585. doi: 10.1128/jb.163.2.580-585.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Miller V. L., Mekalanos J. J. Synthesis of cholera toxin is positively regulated at the transcriptional level by toxR. Proc Natl Acad Sci U S A. 1984 Jun;81(11):3471–3475. doi: 10.1073/pnas.81.11.3471. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Neill R. J., Ivins B. E., Holmes R. K. Synthesis and secretion of the plasmid-coded heat-labile enterotoxin of Escherichia coli in Vibrio cholerae. Science. 1983 Jul 15;221(4607):289–291. doi: 10.1126/science.6857285. [DOI] [PubMed] [Google Scholar]
  20. Newland J. W., Green B. A., Holmes R. K. Transposon-mediated mutagenesis and recombination in Vibrio cholerae. Infect Immun. 1984 Aug;45(2):428–432. doi: 10.1128/iai.45.2.428-432.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Pearson G. D., Mekalanos J. J. Molecular cloning of Vibrio cholerae enterotoxin genes in Escherichia coli K-12. Proc Natl Acad Sci U S A. 1982 May;79(9):2976–2980. doi: 10.1073/pnas.79.9.2976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Saunders D. W., Kubala G. J., Vaidya A. B., Bramucci M. G. Evidence indicating that the cholera toxin structural genes of Vibrio cholerae RJ1 and 3083-2 are between met and trp. Infect Immun. 1983 Oct;42(1):427–430. doi: 10.1128/iai.42.1.427-430.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Southern E. Gel electrophoresis of restriction fragments. Methods Enzymol. 1979;68:152–176. doi: 10.1016/0076-6879(79)68011-4. [DOI] [PubMed] [Google Scholar]
  24. Thomas P. S. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201–5205. doi: 10.1073/pnas.77.9.5201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Vasil M. L., Holmes R. K., Finkelstein R. A. Conjugal transfer of a chromosomal gene determining production of enterotoxin in vibrio cholerae. Science. 1975 Mar 7;187(4179):849–850. doi: 10.1126/science.1114331. [DOI] [PubMed] [Google Scholar]
  26. White B. A., Bancroft F. C. Cytoplasmic dot hybridization. Simple analysis of relative mRNA levels in multiple small cell or tissue samples. J Biol Chem. 1982 Aug 10;257(15):8569–8572. [PubMed] [Google Scholar]

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