Abstract
A 46-kilodalton (kDa) polypeptide was immunoprecipitated from radiolabeled extracts of human cell lines infected with Mycoplasma hyorhinis by murine monoclonal antibodies PF/2A and ML77. Both of these antibodies also reacted in an enzyme-linked immunosorbent assay (ELISA) with M. hyorhinis cells and with human and nonhuman cell lines infected with M. hyorhinis but failed to react with A7573 cells infected with any of 10 other species of the order Mycoplasmatales. PF/2A also reacted in the ELISA with certain human cell lines that were demonstrated to be free of mycoplasma infection. From extracts of these lines, a polypeptide antigen that appeared as a 24-kDa doublet on polyacrylamide gels was immunoprecipitated by PF/2A. When the PF/2A-reactive human cell lines were infected by M. hyorhinis, both the 46- and 24-kDa antigens were immunoprecipitated by PF/2A. ML77 did not react in the ELISA with any noninfected human cells tested and failed to immunoprecipitate a 24-kDa component from any human cells. In Western blotting analyses of extracts of M. hyorhinis cells, both PF/2A and ML77 stained a 46-kDa band. PF/2A also stained 24-kDa bands in Western blotting analyses of reactive human cells and M. hyorhinis cells, although a 24-kDa component was not precipitated from extracts of M. hyorhinis cells by PF/2A.
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